Coordinated regulation of heterochromatin inheritance by Dpb3–Dpb4 complex

He, Haijin; Li, Yang; Dong, Qingyu; Chang, An Yun; Gao, Feng; Chi, Zhongxuan; Su, Min; Zhang, Faben; Ban, Hyoju; Martienssen, Rob; Chen, Yu Hang; Li, Fēi

doi:10.1073/pnas.1712961114

Cited by 53 publications

(57 citation statements)

References 40 publications

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“…Pol α was also important for heterochromatin silencing maintenance in fission yeast and the restoration of repressive histone marks in plants [159,160]. Similarly, it has been shown that both the Pol ε catalytic subunit Pol2 and the regulatory subunits Dpb3-Dpb4 are important for the maintenance of epigenetic states in subtelomeric regions and heterochromatin boundary specificity in budding yeast [161,162]. dpb3Δ and dpb4Δ cells also showed silencing loss at mating-type loci [158].…”

Section: Dna Polymerases: Key Players In Histone Segregation On Daughmentioning

confidence: 99%

The replisome guides nucleosome assembly during DNA replication

Zhang

Feng

2020

Cell Biosci

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Nucleosome assembly during DNA replication is tightly coupled to ongoing DNA synthesis. This process, termed DNA replication-coupled (RC) nucleosome assembly, is essential for chromatin replication and has a great impact on both genome stability maintenance and epigenetic inheritance. This review discusses a set of recent findings regarding the role of replisome components contributing to RC nucleosome assembly. Starting with a brief introduction to the factors involved in nucleosome assembly and some aspects of the architecture of the eukaryotic replisome, we discuss studies from yeast to mammalian cells and the interactions of replisome components with histones and histone chaperones. We describe the proposed functions of replisome components during RC nucleosome assembly and discuss their impacts on histone segregation and implications for epigenetic inheritance.

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Section: Dna Polymerases: Key Players In Histone Segregation On Daughmentioning

confidence: 99%

The replisome guides nucleosome assembly during DNA replication

Zhang

Feng

2020

Cell Biosci

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“…Pol ε plays crucial roles in chromosome replication, cell cycling, and the repair of damaged DNA 17 . Both Dpb3 and Dpb4 are non-essential proteins that form a heterodimeric complex and bind to double-stranded DNA [18][19][20] . The Dpb3-Dpb4 complex physically associates with the Pol ε catalytic subunit, Cdc20, and also interacts with proteins that are important for heterochromatin assembly.…”

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confidence: 99%

Characterization of the CCAAT-binding transcription factor complex in the plant pathogenic fungus Fusarium graminearum

Kim

Nam

Park

et al. 2020

Sci Rep

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the ccAAt sequence is a ubiquitous cis-element of eukaryotic promoters, and genes containing CCAAT sequences have been shown to be activated by the CCAAT-binding transcription factor complex in several eukaryotic model organisms. In general, CCAAT-binding transcription factors form heterodimers or heterotrimeric complexes that bind to ccAAt sequences within the promoters of target genes and regulate various cellular processes. To date, except Hap complex, CCAAT-binding complex has been rarely reported in fungi. In this study, we characterized two CCAAT-binding transcription factors (Fct1 and Fct2) in the plant pathogenic fungus Fusarium graminearum. Previously, FCT1 and FCT2 were shown to be related to DnA damage response among eight ccAAt-binding transcription factors in F. graminearum. We demonstrate that the nuclear CCAAT-binding complex of F. graminearum has important functions in various fungal developmental processes, not just DNA damage response but virulence and mycotoxin production. Moreover, the results of biochemical and genetic analyses revealed that Fct1 and Fct2 may form a complex and play distinct roles among the eight CCAAT-binding transcription factors encoded by F. graminearum. To the best of our knowledge, the results of this study represent a substantial advancement in our understanding of the molecular mechanisms underlying the functions of CCAAT-binding factors in eukaryotes. Gene expression is primarily orchestrated by a set of transcription factors that bind to cis-elements in promoter regions 1. In addition to the TATA-box, the CCAAT sequence is a ubiquitous cis-element of eukaryotic promoters that is present in the promoters of approximately 30% of eukaryotic genes 2. Therefore, genes carrying a CCAAT-box are known to be primarily activated by conserved CCAAT-binding complexes in model eukaryotic organisms 3. The CCAAT-binding complex (CBC) typically consists of heterotrimeric core subunits and regulates primary/ secondary metabolism, development, stress responses, and virulence in animals, plants, and fungi 4-7. The heme activator protein (HAP) complex, which is also termed nuclear factor Y (NF-Y) or CCAAT-binding factor (CBF), was the first identified and is the most well studied CBC in various eukaryotic organisms. The Saccharomyces cerevisiae Hap complex consists of three essential CCAAT-binding factors (Hap2p, Hap3p, and Hap5p) that are indispensable for CCAAT-binding activity 8,9 , orthologues of which (NF-YA/CBF-B, NF-YB/CBF-A, and NF-YC/ CBF-C, respectively) also comprise the mammalian CCAAT complex (NF-Y/CBF) 10. Core elements of Hap3 and Hap5 display amino acid sequence similarities to the histone fold motifs of histones H2B and H2A, respectively, which are responsible for heterodimeric interactions. Another essential element of the complex, Hap2, contains a subunit association domain that allows for heterotrimer formation and nuclear localization signals (NLS) 11,12. After the assembly of Hap2, Hap3, and Hap5, the heterotrimeric complex then recruits Hap4, an additional compon...

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“…Recent studies uncovered strand-specific pathways of parental histone recycling in nascent chromatin (He et al, 2017;Gan et al, 2018;Petryk et al, 2018;Yu et al, 2018). The transfer of parental histones H3-H4 to the lagging strand relies on the synergistic action of the histone chaperone Mini-Chromosome Maintenance Protein 2 (MCM2), a subunit of the MCM helicase, chromosome transmission fidelity 4 (CTF4), and the lagging strand-specific primase DNA polymerase α (Pol-α) (Huang et al, 2015;Gan et al, 2018;Petryk et al, 2018; Figure 1B).…”

Section: Recycling Of H3k27me3-marked Nucleosomes and Incorporation Omentioning

confidence: 99%

“…CTF4 anchors Pol-α in the vicinity of the MCM helicase, thus enabling the transfer of parental histones H3-H4 from MCM2 to the lagging stand via Pol-α. The transfer of parental histones H3-H4 to the leading strand depends on Dpb3-4, a heterodimer associated with the leading strand-specific DNA polymerase (Pol-) (He et al, 2017;Yu et al, 2018; Figure 1B ). Interestingly, asymmetric inheritance of parental histones H3-H4 in fission yeast lacking either Dpb3 or Dpb4 results in loss of heterochromatin integrity and transcriptional activation, which emphasizes the functional role of epigenetic inheritance in the maintenance of genome stability and transcriptional programs (He et al, 2017;Yu et al, 2018).…”

Section: Recycling Of H3k27me3-marked Nucleosomes and Incorporation Omentioning

confidence: 99%

Mitotic Inheritance of PRC2-Mediated Silencing: Mechanistic Insights and Developmental Perspectives

2020

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Maintenance of gene repression by Polycomb Repressive Complex 2 (PRC2) that catalyzes the trimethylation of histone H3 at lysine 27 (H3K27me3) is integral to the orchestration of developmental programs in most multicellular eukaryotes. Faithful inheritance of H3K27me3 patterns across replication ensures the stability of PRC2mediated transcriptional silencing over cell generations, thereby safeguarding cellular identities. In this review, we discuss the molecular and mechanistic principles that underlie H3K27me3 restoration after the passage of the replication fork, considering recent advances in different model systems. In particular, we aim at emphasizing parallels and differences between plants and other organisms, focusing on the recycling of parental histones and the replenishment of H3K27me3 patterns post-replication thanks to the remarkable properties of the PRC2 complex. We then discuss the necessity for fine-tuning this genuine epigenetic memory system so as to allow for cell fate and developmental transitions. We highlight recent insights showing that genome-wide destabilization of the H3K27me3 landscape during chromatin replication participates in achieving this flexible stability and provides a window of opportunity for subtle transcriptional reprogramming.

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Coordinated regulation of heterochromatin inheritance by Dpb3–Dpb4 complex

Cited by 53 publications

References 40 publications

The replisome guides nucleosome assembly during DNA replication

The replisome guides nucleosome assembly during DNA replication

Characterization of the CCAAT-binding transcription factor complex in the plant pathogenic fungus Fusarium graminearum

Mitotic Inheritance of PRC2-Mediated Silencing: Mechanistic Insights and Developmental Perspectives

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