Coordinated Gene Regulation in the Initial Phase of Salt Stress Adaptation

Vanacloig-Pedros, Elena; Bets-Plasencia, Carolina; Pascual-Ahuir, Amparo; Proft, Markus

doi:10.1074/jbc.m115.637264

Cited by 20 publications

(15 citation statements)

References 49 publications

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“…Although any free or engaged molecules found at genes connected to need-to-beinduced genes could provide transcription machinery and coregulators, we speculate that highly transcribed genes could be used as a reservoir within each ecosystem. Indeed, Pol II accumulates at the promoter of early response genes although its levels decrease at housekeeping genes during stress responses (Cook and O'Shea, 2012;Vanacloig-Pedros et al, 2015) For example, in yeast, salt stress adaptation is directly linked to a loss of gene expression from highly transcribed genes (Vanacloig-Pedros et al, 2015). In mammalian cells, housekeeping genes are well connected with other cell-type-specific and stress response genes (Rao et al, 2014).…”

Section: Molecular Cellmentioning

confidence: 99%

Defining the Transcriptional Ecosystem

Silveira

Bilodeau

2018

Molecular Cell

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Section: Molecular Cellmentioning

confidence: 99%

Defining the Transcriptional Ecosystem

Silveira

Bilodeau

2018

Molecular Cell

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“…2 , a randomly selected sample). Although the glycolytic enzymes, such as ENO2, that play an important role in regulating salt stress were widely studied [ 9 , 10 ], little attention has been paid to the mechanism of the knock-out of ENO2 that results in damage and apoptosis of leaves in salt treatment. The physiological modulation of the plant response to salinity relies on the proteins involved in signaling, changes in gene expression, and protein metabolism.…”

Section: Resultsmentioning

confidence: 99%

ENO2 knock-out mutants in Arabidopsis modify the regulation of the gene expression response to NaCl stress

Chen

Zhang

et al. 2018

Mol Biol Rep

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There is a growing awareness that some dual-function enzymes may provide a directly evidence that metabolism could feed into the regulation of gene expression via metabolic enzymes. However, the mechanism by which metabolic enzymes control gene expression to optimize plant stress responses remains largely unknown in Arabidopsis thaliana. LOS2/ENO2 is a bifunctional gene transcribed a functional RNA that translates a full-length version of the ENO2 protein and a truncated version of the MBP-1 protein. Here, we report that eno2 negatively regulates plant tolerance to salinity stress. NaCl treatment caused the death of the mutant eno2/eno2 homozygote earlier than the wild type (WT) Arabidopsis. To understand the mechanism by which the mutant eno2 had a lower NaCl tolerance, an analysis of the expressed sequence tag (EST) dataset from the WT and mutant eno2 Arabidopsis was conducted. Firstly, the most identified up- and down-regulated genes are senescence-associated gene 12 (SAG12) and isochorismate mutase-related gene, which are associated with salicylic acid (SA) inducible plant senescence and endogenous SA synthesis, respectively. Secondly, the differentially regulated by salt stress genes in mutant eno2 are largely enriched Gene Ontology(GO) terms associated with various kinds of response to stimulations. Thirdly, in the Kyoto Encyclopedia of Genes and Genomes (KEGG) mapping, we find that knocking out ENO2-influenced genes were most enriched into metabolite synthesis with extra plant-pathogen interaction pathway and plant hormone signal transduction pathway. Briefly, with the translation shifting function, LOS2/ENO2 not only influenced the genes involved in SA synthesis and transduction, but also influenced genes that participate in metabolite synthesis in cytoplasm and gene expression variation in nuclear under salt stress.Electronic supplementary materialThe online version of this article (10.1007/s11033-018-4292-7) contains supplementary material, which is available to authorized users.

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“…intermediates. Consequently, less efficiently fermentable carbon sources strongly interfere with glycerol biosynthesis and salt stress adaptation and resistance (Vanacloig-Pedros et al, 2015). Conversely, moderate NaCl concentrations significantly interfere with the sugar uptake in yeast cultures (Wei et al, 1982), and sugar transporter genes are among the most highly induced genes upon salt stress (Posas et al, 2000;Rep et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Multilayered control of peroxisomal activity upon salt stress in Saccharomyces cerevisiae

Manzanares-Estreder

Espí-Bardisa

Alarcón

et al. 2017

Molecular Microbiology

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Peroxisomes are dynamic organelles and the sole location for fatty acid β-oxidation in yeast cells. Here, we report that peroxisomal function is crucial for the adaptation to salt stress, especially upon sugar limitation. Upon stress, multiple layers of control regulate the activity and the number of peroxisomes. Activated Hog1 MAP kinase triggers the induction of genes encoding enzymes for fatty acid activation, peroxisomal import and β-oxidation through the Adr1 transcriptional activator, which transiently associates with genes encoding fatty acid metabolic enzymes in a stress- and Hog1-dependent manner. Moreover, Na and Li stress increases the number of peroxisomes per cell in a Hog1-independent manner, which depends instead of the retrograde pathway and the dynamin related GTPases Dnm1 and Vps1. The strong activation of the Faa1 fatty acyl-CoA synthetase, which specifically localizes to lipid particles and peroxisomes, indicates that adaptation to salt stress requires the enhanced mobilization of fatty acids from internal lipid stores. Furthermore, the activation of mitochondrial respiration during stress depends on peroxisomes, mitochondrial acetyl-carnitine uptake is essential for salt resistance and the number of peroxisomes attached to the mitochondrial network increases during salt adaptation, which altogether indicates that stress-induced peroxisomal β-oxidation triggers enhanced respiration upon salt shock.

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Coordinated Gene Regulation in the Initial Phase of Salt Stress Adaptation

Cited by 20 publications

References 49 publications

Defining the Transcriptional Ecosystem

Defining the Transcriptional Ecosystem

ENO2 knock-out mutants in Arabidopsis modify the regulation of the gene expression response to NaCl stress

Multilayered control of peroxisomal activity upon salt stress in Saccharomyces cerevisiae

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