Coordinated Expression of Astaxanthin Biosynthesis Genes for Improved Astaxanthin Production in <i>Escherichia coli</i>

Gong, Zhongkuo; Wang, Honglei; Tang, Ji‐Xin; Bi, Chen; Li, Qingyan; Zhang, Xueli

doi:10.1021/acs.jafc.0c05379

Cited by 48 publications

(55 citation statements)

References 45 publications

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“…The astaxanthin titer obtained by E. coli AST-4AS is lower than the highest value (1.18 g/L) ever reported [16]. The highest astaxanthin titer was obtained by high cell-density culture with OD600 of 469 [16]. However, the astaxanthin content (9.21 ± 0.12 mg/g DCW) obtained by E. coli AST-4AS is higher than that (7.8 mg/g DCW) reported in this literature [16].…”

Section: Alecontrasting

confidence: 56%

“…E. coli AST-4AS produced astaxanthin of 311.20 ± 0.36 mg/L and 9.21 ± 0.12 mg/g DCW with a productivity of 5.55 mg/L/h, which was a 53.7% increase in the astaxanthin titer compared to E. coli AST-4. The astaxanthin titer obtained by E. coli AST-4AS is lower than the highest value (1.18 g/L) ever reported [16]. The highest astaxanthin titer was obtained by high cell-density culture with OD600 of 469 [16].…”

Section: Alementioning

confidence: 60%

“…The highest astaxanthin titer was obtained by high cell-density culture with OD600 of 469 [ 16 ]. However, the astaxanthin content (9.21 ± 0.12 mg/g DCW) obtained by E. coli AST-4AS is higher than that (7.8 mg/g DCW) reported in this literature [ 16 ]. Moreover, the astaxanthin ratio in the total carotenoid reached about 100% in E. coli AST-4AS (Additional file 1 : Fig.…”

Section: Resultsmentioning

confidence: 99%

“…To lower the cost, many studies focused on the production of astaxanthin from an inexpensive carbon source using microbes. Escherichia coli [8][9][10][11][12][13][14][15][16][17], Saccharomyces cerevisiae [18][19][20][21][22][23][24], Corynebacterium glutamicum [25] and Yarrowia lipolytica [26] have been used as host…”

Section: Astaxanthinmentioning

confidence: 99%

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Adaptive laboratory evolution and shuffling of Escherichia coli to enhance its tolerance and production of astaxanthin

Zhou

Liu

2022

Biotechnol Biofuels

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Background Astaxanthin is one of the strongest antioxidants in nature and has been widely used in aquaculture, food, cosmetic and pharmaceutical industries. Numerous stresses caused in the process of a large scale-culture, such as high acetate concentration, high osmolarity, high level of reactive oxygen species, high glucose concentration and acid environment, etc., limit cell growth to reach the real high cell density, thereby affecting astaxanthin production. Results We developed an adaptive laboratory evolution (ALE) strategy to enhance the production of chemicals by improving strain tolerance against industrial fermentation conditions. This ALE strategy resulted in 18.5% and 53.7% increases in cell growth and astaxanthin production in fed-batch fermentation, respectively. Whole-genome resequencing showed that 65 mutations with amino acid substitution were identified in 61 genes of the shuffled strain Escherichia coli AST-4AS. CRISPR interference (CRISPRi) and activation (CRISPRa) revealed that the shuffled strain with higher astaxanthin production may be associated with the mutations of some stress response protein genes, some fatty acid biosynthetic genes and rppH. Repression of yadC, ygfI and rcsC, activation of rnb, envZ and recC further improved the production of astaxanthin in the shuffled strain E. coli AST-4AS. Simultaneous deletion of yadC and overexpression of rnb increased the production of astaxanthin by 32% in the shuffled strain E. coli AST-4AS. Conclusion This ALE strategy will be powerful in engineering microorganisms for the high-level production of chemicals.

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Section: Alecontrasting

confidence: 56%

Section: Alementioning

confidence: 60%

Section: Resultsmentioning

confidence: 99%

Section: Astaxanthinmentioning

confidence: 99%

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Adaptive laboratory evolution and shuffling of Escherichia coli to enhance its tolerance and production of astaxanthin

Zhou

Liu

2022

Biotechnol Biofuels

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“…In such a pathway engineering research of these carotenoids, bacterial carotenoid biosynthesis ( crt ) genes have been utilized for their functional expression, e.g. crtE, crtB, crtI, crtY, crtZ and crtW genes have been used for astaxanthin production ( 15 , 19 , 20 ). Alternatively, few reports have described lutein biosynthesis in the E. coli host by the functional expression of carotenogenic genes that were derived from a plant (liverwort) (genes of lutein biosynthesis from lycopene) and bacteria (the crtE, crtB and crtI genes for lycopene biosynthesis) ( 21 , 22 ).…”

Section: Introductionmentioning

confidence: 99%

Pathway engineering for high-yield production of lutein in Escherichia coli

et al. 2021

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Lutein is an industrially important carotenoid pigment, which is essential for photoprotection and photosynthesis in plants. It is crucial for maintaining human health due to its protective ability from ocular diseases. However, its pathway engineering research has scarcely been performed for microbial production using heterologous hosts, such as Escherichia coli, since the engineering of multiple genes are required there. These genes, which include tricky key carotenoid biosynthesis genes typically derived from plants, encode two sorts of cyclases (lycopene ε- and β-cyclase) and cytochrome P450 CYP97C. In this study, upstream genes effective for the increase in carotenoid amounts, such as isopentenyl diphosphate (IPP) isomerase (IDI) gene, were integrated into the E. coli JM101 (DE3) genome. The most efficient set of the key genes (MpLCYe, MpLCYb, and MpCYP97C) was selected from among corresponding genes derived from various plant (or bacterial) species using E. coli that had accumulated carotenoid substrates. Furthermore, to optimize the production of lutein in E. coli, we introduced several sorts of plasmids that contained some of the multiple genes into the genome-inserted strain and compared lutein productivity. Finally, we achieved 11 mg/L as lutein yield at a mini jar level. Here, high-yield production of lutein was successfully performed using E. coli through approaches of pathway engineering. The findings obtained here should be a base reference for substantial lutein production with microorganisms in the future.

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Comparative transcriptome analysis reveals the redirection of metabolic flux from cell growth to astaxanthin biosynthesis in Yarrowia lipolytica

Wang,

Yu,

Feng

et al. 2024

Yeast

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Engineering Yarrowia lipolytica to produce astaxanthin provides a promising route. Here, Y. lipolytica M2 producing a titer of 181 mg/L astaxanthin was isolated by iterative atmospheric and room‐temperature plasma mutagenesis and diphenylamine‐mediated screening. Interestingly, a negative correlation was observed between cell biomass and astaxanthin production. To reveal the underlying mechanism, RNA‐seq analysis of transcriptional changes was performed in high producer M2 and reference strain M1, and a total of 1379 differentially expressed genes were obtained. Data analysis revealed that carbon flux was elevated through lipid metabolism, acetyl‐CoA and mevalonate supply, but restrained through central carbon metabolism in strain M2. Moreover, upregulation of other pathways such as ATP‐binding cassette transporter and thiamine pyrophosphate possibly provided more cofactors for carotenoid hydroxylase and relieved cell membrane stress caused by astaxanthin insertion. These results suggest that balancing cell growth and astaxanthin production may be important to promote efficient biosynthesis of astaxanthin in Y. lipolytica.

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Coordinated Expression of Astaxanthin Biosynthesis Genes for Improved Astaxanthin Production in Escherichia coli

Cited by 48 publications

References 45 publications

Adaptive laboratory evolution and shuffling of Escherichia coli to enhance its tolerance and production of astaxanthin

Adaptive laboratory evolution and shuffling of Escherichia coli to enhance its tolerance and production of astaxanthin

Pathway engineering for high-yield production of lutein in Escherichia coli

Comparative transcriptome analysis reveals the redirection of metabolic flux from cell growth to astaxanthin biosynthesis in Yarrowia lipolytica

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