Coordinated development of muscles and tendons of the<i>Drosophila</i>leg

Soler, C; Daczewska, Małgorzata; Ponte, Jean Philippe Da; Dastugue, B.; Jagla, Krzysztof

doi:10.1242/dev.01527

Cited by 107 publications

(172 citation statements)

References 48 publications

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“…1F,H). Zfh1 expression in embryonic AMPs has also been reported by Sellin et al (Sellin et al, 2009), whereas cut has previously been used to reveal a subset of AMPs associated with larval wing (Sudarsan et al, 2001) and leg imaginal discs (Soler et al, 2006). Despite expressing common markers, the AMPs are heterogenous and differ by the expression of muscle identity genes (Fig.…”

Section: Notch Targets and Repressors Of Myogenic Differentiation Arementioning

confidence: 77%

Drosophila adult muscle precursors form a network of interconnected cells and are specified by the rhomboid-triggered EGF pathway

et al. 2010

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SUMMARYIn Drosophila, a population of muscle-committed stem-like cells called adult muscle precursors (AMPs) keeps an undifferentiated and quiescent state during embryonic life. The embryonic AMPs are at the origin of all adult fly muscles and, as we demonstrate here, they express repressors of myogenic differentiation and targets of the Notch pathway known to be involved in muscle cell stemness. By targeting GFP to the AMP cell membranes, we show that AMPs are tightly associated with the peripheral nervous system and with a subset of differentiated muscles. They send long cellular processes running along the peripheral nerves and, by the end of embryogenesis, form a network of interconnected cells. Based on evidence from laser ablation experiments, the main role of these cellular extensions is to maintain correct spatial positioning of AMPs. To gain insights into mechanisms that lead to AMP cell specification, we performed a gain-of-function screen with a special focus on lateral AMPs expressing the homeobox gene ladybird. Our data show that the rhomboid-triggered EGF signalling pathway controls both the specification and the subsequent maintenance of AMP cells. This finding is supported by the identification of EGF-secreting cells in the lateral domain and the EGF-dependent regulatory modules that drive expression of the ladybird gene in lateral AMPs. Taken together, our results reveal an unsuspected capacity of embryonic AMPs to form a cell network, and shed light on the mechanisms governing their specification and maintenance.

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Section: Notch Targets and Repressors Of Myogenic Differentiation Arementioning

confidence: 77%

Drosophila adult muscle precursors form a network of interconnected cells and are specified by the rhomboid-triggered EGF pathway

et al. 2010

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“…44-5 progeny were selected from a cross of Mef2 30-5 /CyOKrGFP ϫ Mef2 44-5 /CyOKrGFP and switched to the restrictive temperature as described (Baker et al, 2005 Tissue preparation and microscopic analysis for thoracic and leg muscles were essentially as previously described (Soler et al, 2004;Soler and Taylor, 2009). …”

Section: /Mef2mentioning

confidence: 99%

“…We used 1151-Gal4 to drive expression of UASMef2RNAi (15550) in the adult myoblasts of second instar larvae and then continuing throughout adult myogenesis (Anant et al, 1998;Soler et al, 2004). Normally, there are six DLMs in each hemithorax at the end of pupal life.…”

Section: Mef2 Is Required For Adult Fibrillar Musclesmentioning

confidence: 99%

The conserved transcription factor Mef2 has multiple roles in adult Drosophila musculature formation

2012

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SUMMARYMuscle is an established paradigm for analysing the cell differentiation programs that underpin the production of specialised tissues during development. These programs are controlled by key transcription factors, and a well-studied regulator of muscle gene expression is the conserved transcription factor Mef2. In vivo, Mef2 is essential for the development of the Drosophila larval musculature: Mef2-null embryos have no differentiated somatic muscle. By contrast, a similar phenotype has not been seen in analyses of the function of Mef2 genes in other examples of myogenesis. These include using conditional mutant mice, using morpholinos in zebrafish and using hypomorphic mutants in Drosophila adult development. However, we show here that Mef2 is absolutely required for a diverse range of Drosophila adult muscle types. These include the dorso-longitudinal muscles (DLMs), the largest flight muscles, which are produced by tissue remodelling. Furthermore, we demonstrate that Mef2 has temporally separable functions in this remodelling and in muscle maintenance. Drosophila adult muscles are multi-fibre and physiologically diverse, in common with vertebrate skeletal muscles, but in contrast to Drosophila larval muscles. These results therefore establish the importance of Mef2 in multiple roles in examples of myogenesis that have parallels in vertebrates and are distinct from that occurring in Drosophila embryogenesis.

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“…The founder cell pattern also prefigures adult myotube locations, so the weakness and uncoordinated movements seen in nau armGFP larvae and adults may reflect alterations in the normal muscle fiber organization and attachments because of a general disruption of the founder cells throughout development (36,37).…”

mentioning

confidence: 99%

Stereotypic founder cell patterning and embryonic muscle formation in Drosophila require nautilus (MyoD) gene function

Qin¹,

Rong

Paterson³

2007

Proc. Natl. Acad. Sci. U.S.A.

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nautilus is the only MyoD-related gene in Drosophila. Nautilus expression begins around stage 9 at full germ-band extension in a subset of mesodermal cells organized in a stereotypic pattern in each hemisegment. The muscle founder cell marker Duf-LacZ, produced by the enhancer trap line rP298LacZ, is coexpressed in numerous Nautilus-positive cells when founders first appear. Founders entrain muscle identity through the restricted expression of transcription factors such as S59, eve, and Kr, all of which are observed in subsets of the nautilus expressing founders. We inactivated the nautilus gene using homology-directed gene targeting and Gal4/UAS regulated RNAi to determine whether loss of nautilus gene activity affected founder cell function. Both methods produced a range of defects that included embryonic muscle disruption, reduced viability and female sterility, which could be rescued by hsp70-nautilus cDNA transgenes. Our results demonstrate Nautilus expression marks early founders that give rise to diverse muscle groups in the embryo, and that nautilus gene activity is required to seed the correct founder myoblast pattern that prefigures the muscle fiber arrangement during embryonic development.muscle organization ͉ myogenic factors ͉ pattern determination

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Coordinated development of muscles and tendons of theDrosophilaleg

Cited by 107 publications

References 48 publications

Drosophila adult muscle precursors form a network of interconnected cells and are specified by the rhomboid-triggered EGF pathway

Drosophila adult muscle precursors form a network of interconnected cells and are specified by the rhomboid-triggered EGF pathway

The conserved transcription factor Mef2 has multiple roles in adult Drosophila musculature formation

Stereotypic founder cell patterning and embryonic muscle formation in Drosophila require nautilus (MyoD) gene function

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