Coordinated Control of dCTCF and gypsy Chromatin Insulators in Drosophila

Gerasimova, T. I.; Lei, Elissa P.; Bushey, Ashley M.; Corcés, Victor G.

doi:10.1016/j.molcel.2007.09.024

Cited by 126 publications

(196 citation statements)

References 29 publications

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“…As CP190 and Mod(mdg4) are involved in the activity of Su(Hw) and dCTCF insulators, 30 it has been suggested that the BTB domains of these proteins are involved in specific interaction between insulators. Here, we have found that the BTB domains of these proteins are completely different.…”

Section: Discussionmentioning

confidence: 99%

“…20 Interestingly, Su(Hw) also interacts with the BTB-containing CP190 protein. 28 This protein is essential for enhancer blocking of the Su (Hw) insulator and also interacts with other Drosophila insulator proteins, dCTCF 29,30 and BEAF. 31 Although the BTB domain of CP190 does not belong to the ttk group, it is suggested to play a significant role in supporting long-distance interactions between insulators.…”

Section: Introductionmentioning

confidence: 99%

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Drosophila BTB/POZ Domains of “ttk Group” Can Form Multimers and Selectively Interact with Each Other

Bonchuk

Denisov

Georgiev

et al. 2011

Journal of Molecular Biology

104

109

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Edited by M. GottesmanKeywords: GAGA; BTB domain; Mod(mdg4); CP190; chromatin insulatorThe BTB (bric-a-brac, tramtrack and broad complex)/POZ (poxvirus and zinc finger) domain is a conserved protein-protein interaction motif contained in a variety of transcription factors involved in development, chromatin remodeling, insulator activity, and carcinogenesis. All wellstudied mammalian BTB domains form obligate homodimers and, rarely, tetramers. Only the BTB domain of the Drosophila GAGA factor (GAF) has been shown to exist as higher-order multimers. The BTB domain of GAF belongs to the "ttk group" that contains several highly conserved sequences not found in other BTB domains. Here, we have shown by size-exclusion chromatography, chemical cross-linking, and nondenaturing PAGE that four additional BTB domains of the ttk group-Batman, Mod(mdg4), Pipsqueak, and Tramtrack-can form multimers, like GAF. Interestingly, the BTB domains of GAF and Batman have formed a wide range of complexes and interacted in the yeast two-hybrid assay with other BTB domains tested. In contrast, the BTB domains of Mod(mdg4), Pipsqueak, and Tramtrack have formed stable high-order multimer complexes and failed to interact with each other. The BTB domain of Drosophila CP190 protein does not belong to the ttk group. This BTB domain has formed stable dimers and has not interacted with domains of the ttk group. Previously, it was suggested that GAF oligomerization into higher-order complexes facilitates long-range activation by providing a protein bridge between an enhancer and a promoter. Unexpectedly, experiments in the Drosophila model system have not supported the role of GAF in organization of longdistance interaction between the yeast GAL4 activator and the white promoter.© 2011 Elsevier Ltd. All rights reserved. IntroductionThe BTB (bric-a-brac, tramtrack and broad complex) domain [also known as the POZ (poxvirus and zinc finger) domain] is a versatile protein-protein interaction motif that participates in a wide range of cellular functions, including transcription regulation (for review, see Ref. 1 ). Transcription factors with a BTB domain at the N-terminus comprise a large important class of molecules involved in development and carcinogenesis. As shown in crystallographic studies,

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Drosophila BTB/POZ Domains of “ttk Group” Can Form Multimers and Selectively Interact with Each Other

Bonchuk

Denisov

Georgiev

et al. 2011

Journal of Molecular Biology

104

109

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“…Our laboratory studies the role of insulators in chromatin organization and gene expression (Geyer et al 1988;Bushey et al 2008). Previous studies have shown that Drosophila insulator proteins are present at hundreds of sites throughout the genome that colocalize to distinct foci predominantly around the nuclear periphery in diploid cells (Ramos et al 2006;Gerasimova et al 2007). A number of proteins have been identified that interact with insulator DNA but additional components may be required to explain all the properties of these sequences (Pai et al 2004;Capelson and Corces 2005;Lei and Corces 2006;Bushey et al 2009).…”

Section: Choice Of a Research Topic For The Rise Programmentioning

confidence: 99%

Opening Pathways for Underrepresented High School Students to Biomedical Research Careers: The Emory University RISE Program

Rohrbaugh

Corcés

2011

Genetics

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Increasing the college graduation rates of underrepresented minority students in science disciplines is essential to attain a diverse workforce for the 21st century. The Research Internship and Science Education (RISE) program attempts to motivate and prepare students from the Atlanta Public School system, where underrepresented minority (URM) students comprise a majority of the population, for biomedical science careers by offering the opportunity to participate in an original research project. Students work in a research laboratory from the summer of their sophomore year until graduation, mentored by undergraduate and graduate students and postdoctoral fellows (postdocs). In addition, they receive instruction in college-level biology, scholastic assessment test (SAT) preparation classes, and help with the college application process. During the last 4 yr, RISE students have succeeded in the identification and characterization of a series of proteins involved in the regulation of nuclear organization and transcription. All but 1 of 39 RISE students have continued on to 4-year college undergraduate studies and 61% of those students are currently enrolled in sciencerelated majors. These results suggest that the use of research-based experiences at the high school level may contribute to the increased recruitment of underrepresented students into science-related careers.

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“…In flies, the gypsy insulator bodies are tethered to the nuclear lamina through a complex comprising lamins, 20 and colocalization of dCTCF with these insulator bodies suggests that dCTCF also binds to insulator bodies, in association with the nuclear matrix or the NP. 20,21 To our knowledge, D4Z4 is the first peripheral positioning element described so far in human and couples this original property with boundary activity. By analogy with gypsy, it could be hypothesized that the displacement of our D4Z4-tagged telomeres toward the nuclear periphery is related to similar mechanisms and that in eukaryotes the peripheral location of insulators correspond to the targeting to an insulation-competent subnuclear domain.…”

Section: Multimerization Alters D4z4 Propertiesmentioning

confidence: 99%