Coordinate responses of transcription factors to ecdysone during programmed cell death in the anterior silk gland of the silkworm, <i>Bombyx mori</i>

Sekimoto, Takayuki; Iwami, Masafumi; Sakurai, Shunsuke

doi:10.1111/j.1365-2583.2006.00641.x

Cited by 55 publications

(51 citation statements)

References 45 publications

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“…34 A balancing crosstalk occurs between autophagy and caspase activity in the remodeling fat body, as the inhibition of autophagy induces caspase activity and the inhibition of apoptosis induces autophagy. 35,36 During Bombyx metamorphosis, autophagy and apoptosis occur simultaneously in the silk gland, 37,38 and fat body. 28,29,39 Importantly, we found that autophagy precedes apoptosis in the midgut.…”

Section: Basic Research Papermentioning

confidence: 99%

“…The cells were subsequently incubated with 10 mL of 10 mg/mL RNase A (TIANGEN, M1105) at 37 C for 1 h. Then, 10 mL of 20 mg/mL protease K (TIANGEN, K0012) was added to the reaction for further incubation overnight at 55 C. The following DNA extraction steps were performed according to the method used in silkworm midguts. 40 Finally, 10 mg of genomic DNA was detected on a 2.5% agarose gel and visualized by staining with ethidium bromide.…”

Section: Dna Ladder Detectionmentioning

confidence: 99%

“…The recombinant BmATG5 and BmATG6 were incubated with m-Calpain (Merck, 208713) or Caspase-3 (Enzo, ALX-201-059), respectively, in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0). m-Calpain (10 mg) was incubated with 100 mg recombinant BmATG5 proteins at 25 C for 1 h, and 10 U of Caspase-3 was incubated with 100 mg BmATG6 at 37 C overnight. The reaction was stopped by …”

Section: Bmatg5 and Bmatg6 Cleavage Assaymentioning

confidence: 99%

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BmATG5 and BmATG6 mediate apoptosis following autophagy induced by 20-hydroxyecdysone or starvation

et al. 2016

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Autophagy and apoptosis, which could be induced by common stimuli, play crucial roles in development and disease. The functional relationship between autophagy and apoptosis is complex, due to the dual effects of autophagy. In the Bombyx Bm-12 cells, 20-hydroxyecdysone (20E) treatment or starvationinduced cell death, with autophagy preceding apoptosis. In response to 20E or starvation, BmATG8 was rapidly cleaved and conjugated with PE to form BmATG8-PE; subsequently, BmATG5 and BmATG6 were cleaved into BmATG5-tN and BmATG6-C, respectively. Reduction of expression of BmAtg5 or BmAtg6 by RNAi decreased the proportion of cells undergoing both autophagy and apoptosis after 20E treatment or starvation. Overexpression of BmAtg5 or BmAtg6 induced autophagy but not apoptosis in the absence of the stimuli, but promoted both autophagy and apoptosis induced by 20E or starvation. Notably, overexpression of cleavage site-deleted BmAtg5 or BmAtg6 increased autophagy but not apoptosis induced by 20E or starvation, whereas overexpression of BmAtg5-tN and BmAtg6-C was able to directly trigger apoptosis or promote the induced apoptosis. In conclusion, being cleaved into BmATG5-tN and BmATG6-C, BmATG5 and BmATG6 mediate apoptosis following autophagy induced by 20E or starvation in Bombyx Bm-12 cells, reflecting that autophagy precedes apoptosis in the midgut during Bombyx metamorphosis.

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Section: Basic Research Papermentioning

confidence: 99%

Section: Dna Ladder Detectionmentioning

confidence: 99%

Section: Bmatg5 and Bmatg6 Cleavage Assaymentioning

confidence: 99%

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BmATG5 and BmATG6 mediate apoptosis following autophagy induced by 20-hydroxyecdysone or starvation

et al. 2016

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“…It has been reported that Ec is responsible for embryo development, larval molts, pupation, and metamorphosis in Drosophila, Bombyx mori and other arthropods (Riddiford 1993, Subramoniam 2000, Sekimoto et al 2006. The Ec is known to regulate a number of biological processes by coordinating with juvenile hormone (JH; Dubrovsky 2005) and cross talk between them has been reported in Daphnia and other arthropods (Mu & LeBlanc 2004).…”

Section: Introductionmentioning

confidence: 99%

Cloning and characterization of the ecdysone receptor and ultraspiracle protein from the water flea Daphnia magna

Kato¹,

Kobayashi²,

Oda³

et al. 2007

Journal of Endocrinology

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cDNAs encoding the ecdysone receptor (EcR) and ultraspiracle (USP) protein were cloned from the water flea Daphnia magna (Crustacea: Cladocera). The deduced EcR and USP amino acid sequences showed a high degree of homology to those of other crustaceans as well as insects. We isolated three isoforms of EcR that differ in the A/B domain. Quantitative PCR analysis indicated differing temporal expression patterns of the EcR isoforms during the molting period and demonstrated that the expression of one subtype correlated well with the timing of molt. Using cDNAs encoding EcR and USP, we constructed a Daphnia EcR/USP reporter based on a two-hybrid system.The gene fusions encoded the EcR ligand-binding domain (LBD) fused to the Gal4 DNA-binding domain, and the USP-LBD fused to the Vp16 activation domain. These chimeric genes were transfected with a luciferase reporter gene. Dose-dependent activation of the reporter gene could be observed when transfectants were exposed to Ec and other chemicals known to have Ec-like activities. This two-hybrid system may represent a useful reporter system for further examination of hormonal and chemical effects on Daphnia at the molecular level.

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“…Metamorphosis of holometabolous insects carries a radical reconstruction of the body planes to a replacement of the larval tissues by adult tissues and structures. Processes that drive these changes are controlled by the insect steroid molting hormone ecdysone (Jiang et al, 1997(Jiang et al, , 2000Sekimoto et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Programmed cell death in salivary glands of Drosophila arizonae and Drosophila mulleri

Ianella¹,

Azeredo-Oliveira²,

Itoyama³

2008

Genet. Mol. Res.

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ABSTRACT. Programmed cell death (PCD) in insect metamorphosis assumes a great diversity of morphology and controlling processes that are still not well understood. With the objective of obtaining information about the PCD process, salivary glands of Drosophila arizonae and D. mulleri were studied during larval-pupal development. From the results, it can be concluded that the type of the PCD that occurs in these organs is morphologically typical of apoptosis (formation of apoptotic nuclei, followed by fragmentation into apoptotic bodies). Histolysis happens in both species, between 22 and 23 h after pupation. There were no significant differences between the species studied. Apoptosis does not occur simultaneously in all cells. Cytoplasmic acid phosphatase activity gradually increases during development, suggesting the existence of acid phosphatases that are only expressed during the apoptotic stage. Twenty hours after pupation, salivary glands already show biochemical alterations relative to nuclear permeability such as acidification, possibly due to the fusion of lysosomes with the nucleus a few hours before apoptosis. Autophagy seems to act together with apoptosis and has a secondary role in cell death.

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Coordinate responses of transcription factors to ecdysone during programmed cell death in the anterior silk gland of the silkworm, Bombyx mori

Cited by 55 publications

References 45 publications

BmATG5 and BmATG6 mediate apoptosis following autophagy induced by 20-hydroxyecdysone or starvation

BmATG5 and BmATG6 mediate apoptosis following autophagy induced by 20-hydroxyecdysone or starvation

Cloning and characterization of the ecdysone receptor and ultraspiracle protein from the water flea Daphnia magna

Programmed cell death in salivary glands of Drosophila arizonae and Drosophila mulleri

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