Coordinate regulation of DNA methyltransferase expression during oogenesis

Lucifero, Diana; Salle, Sophie; Bourc’his, Déborah; Martel, Josée; Bestor, Timothy H.; Trasler, Jacquetta M.

doi:10.1186/1471-213x-7-36

Cited by 104 publications

(80 citation statements)

References 48 publications

(99 reference statements)

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“…However, Dicer deficiency barely affects TE expression levels (Murchison et al, 2007;Watanabe et al, 2008). Moreover, TEs are naturally hypomethylated and expressed in mature oocytes (Peaston et al, 2004;Lucifero et al, 2007), suggesting that these cells are apt to tolerate the presence of TE transcripts. Active cell division is an important requirement for TE transposition, and the long meiotic arrest endured by oocytes may constitute an innate barrier to the completion of the full TE cycle (Shi et al, 2007).…”

Section: Te Silencing In the Germlinementioning

confidence: 99%

Transposable elements in the mammalian germline: a comfortable niche or a deadly trap?

2010

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Retrotransposable elements comprise around 50% of the mammalian genome. Their activity represents a constant threat to the host and has prompted the development of adaptive control mechanisms to protect genome architecture and function. To ensure their propagation, retrotransposons have to mobilize in cells destined for the next generation. Accordingly, these elements are particularly well suited to transcriptional networks associated with pluripotent and germinal states in mammals. The relaxation of epigenetic control that occurs in the early developing germline constitutes a dangerous window in which retrotransposons can escape from host restraint and massively expand. What could be observed as risky behavior may turn out to be an insidious strategy developed by germ cells to sense retrotransposons and hold them back in check. Herein, we review recent insights that have provided a detailed picture of the defense mechanisms that concur toward retrotransposon silencing in mammalian genomes, and in particular in the germline. In this lineage, retrotransposons are hit at multiple stages of their life cycle, through transcriptional repression, RNA degradation and translational control. An organized cross-talk between PIWIinteracting small RNAs (piRNAs) and various nuclear and cytoplasmic accessories provides this potent and multilayered response to retrotransposon unleashing in early germ cells.

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Section: Te Silencing In the Germlinementioning

confidence: 99%

Transposable elements in the mammalian germline: a comfortable niche or a deadly trap?

2010

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“…Accumulating data indicate that DNMT3L is important for the dosage-dependent regulation of imprinted genes and transposable element silencing in germ cell development (Bourc'his & Bestor 2004, Kato et al 2007, Lucifero et al 2007, Smallwood et al 2011, Kobayashi et al 2012, Hara et al 2014. During the preimplantation period, Dnmt3l expression is detected in naturally fertilized eggs but subsequently declines until reaching the morula/blastocyst stage, at which it dramatically increases (Vassena et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Dnmt3l-knockout donor cells improve somatic cell nuclear transfer reprogramming efficiency

Liao¹,

Mo²,

Wu³

et al. 2015

Reproduction

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Nuclear transfer (NT) is a technique used to investigate the development and reprogramming potential of a single cell. DNA methyltransferase-3-like, which has been characterized as a repressive transcriptional regulator, is expressed in naturally fertilized egg and morula/blastocyst at pre-implantation stages. In this study, we demonstrate that the use of Dnmt3l-knockout (Dnmt3l-KO) donor cells in combination with Trichostatin A treatment improved the developmental efficiency and quality of the cloned embryos. Compared with the WT group, Dnmt3l-KO donor cell-derived cloned embryos exhibited increased cell numbers as well as restricted OCT4 expression in the inner cell mass (ICM) and silencing of transposable elements at the blastocyst stage. In addition, our results indicate that zygotic Dnmt3l is dispensable for cloned embryo development at pre-implantation stages. In Dnmt3l-KO mouse embryonic fibroblasts, we observed reduced nuclear localization of HDAC1, increased levels of the active histone mark H3K27ac and decreased accumulation of the repressive histone marks H3K27me3 and H3K9me3, suggesting that Dnmt3l-KO donor cells may offer a more permissive epigenetic state that is beneficial for NT reprogramming. Reproduction (2015) 150 245-256

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“…This might be due to the age-related decline in the transcription level of DNMT3L [13]. Additionally, it was reported that the lack of DNMT3L, which participated in the establishment of the maternally imprinted genes, caused the degradation of oocyte quality, and finally brought about the death of early embryos [35]. To sum up, the reduced expression of DNMT3L protein, which was visualized semiquantitatively by its fluorescence intensity, might be another cause of the age-related decline in oocyte quality.…”

Section: Age-related Changes In Expressions Of De Novo Methyltransfermentioning

confidence: 99%

“…In addition, it was reported that the lack of either DNMT3L or DNMT1 in growing oocytes caused increased expression of de novo methyltransferase DNMT3b, suggesting a potential compensation mechanism in which the loss of one of the DNMTs would result in an increase of some others [35]. Our observations that in aging mice, the fluorescence intensities of DNMT1, DNMT3a, and DNMT 3b were higher than in pubertal groups, while that of DNMT3L was much lower, were perhaps related to this compensation mechanism among the DNMT family.…”

Section: Age-related Changes In Expressions Of De Novo Methyltransfermentioning

confidence: 99%

Effects of maternal aging on localizations and expression levels of DNA methyltransferases and chromosome architecture in in-vivo matured mouse oocytes

Tian

Zhang

et al. 2013

Chin. Sci. Bull.

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This paper investigated the age-related changes in the expression patterns of maintenance methyltransferase (DNMT1) and de novo methyltransferases (DNMT3a, 3b, 3L) and the chromosome architecture in in-vivo matured mouse oocytes using two-photon laser-scanning microscope. Our results showed that (1) DNMT1 and DNMT3a, 3b, 3L in the oocytes of pubertal mice were located in the cortical region of oocyte cytoplasm. In aging groups, DNMT1 was also located in the cortical region. However, DNMT3a, 3b, 3L had a relatively wider distribution in the oocyte cytoplasm and appeared near the chromosomes. These differences between pubertal and aging groups suggested that aging might affect DNA methylation; (2) the expression of DNMT1, and DNMT3a, 3b in aging groups increased significantly compared to pubertal groups, while, the expression of DNMT3L decreased. These results might be explained by the compensation mechanism among DNMTs, which might be impervious to aging; (3) aging caused increased errors in the distribution and three-dimensional morphology of chromosomes, including the increased total volume and surface area, the high ratio of height to diameter of a circular cylinder enclosing the chromosomes (H/D). Our work provided morphological information for the studies of age-related decline in oocyte qualities.

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Coordinate regulation of DNA methyltransferase expression during oogenesis

Cited by 104 publications

References 48 publications

Transposable elements in the mammalian germline: a comfortable niche or a deadly trap?

Transposable elements in the mammalian germline: a comfortable niche or a deadly trap?

Dnmt3l-knockout donor cells improve somatic cell nuclear transfer reprogramming efficiency

Effects of maternal aging on localizations and expression levels of DNA methyltransferases and chromosome architecture in in-vivo matured mouse oocytes

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