Coordinate induction of fibronectin, fibronectin receptor, tropomyosin, and actin genes in serum-stimulated fibroblasts

Ryseck, Rolf-Peter; Macdonald-Bravo, H; Zerial, Marino; Bravo, Rodrigo

doi:10.1016/0014-4827(89)90080-3

Cited by 69 publications

(37 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…expression in quiescent rat 3Y1 cells was steeply repressed after growth stimulation by serum or ElA expression (20). This is in contrast to mouse embryo-derived AKR-2B cells (3), NIH 3T3 cells (40), and the human fibrosarcoma cell line HT-1080 (13), in which expression levels of FN, its receptor, and actin increased coordinately shortly after growth stimulation of quiescent cells by serum. FN is a large glycoprotein of the extracellular matrix which plays a central role in cell anchorage through cell-extracellular matrix interaction (24,39,51) and connects intracellular actin filaments to the extracellular matrix by binding to its receptor (7,25).…”

mentioning

confidence: 71%

“…Each ElA deletion mutant was transcribed from the ElA enhancer-promoter (49). As a control, plasmid pUC119 instead of an EIA deletion mutant was cotransfected and the CAT activity (40), and the human fibrosarcoma ccll linc HT-1080 (13 (36a). The same or very closely related proteins (GlOBP) may bind to these sites, since the complex formation between the DNA fragments containing either one of the GC boxes and the El-transformed cell extract was inhibited by the presence of the oligonucleotide containing the G10 stretch as effectively as by the presence of the oligonucleotide containing the same GC box.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

E1A-responsive elements for repression of rat fibronectin gene transcription.

Nakajima¹,

Nakamura²,

Tsunoda³

et al. 1992

Mol. Cell. Biol.

View full text Add to dashboard Cite

The level of fibronectin (FN) gene transcription in resting rat 3Y1 cells is very high but decreases steeply after growth stimulation by serum or by the induction of EIA expression. To study the mechanism of this ElA-mediated down-regulation, the 5' flanking regions of the FN gene with various deletions and substitutions were fused to the Escherichia coli chloramphenicol acetyltransferase (CAT) gene and introduced into resting 3Y1 cells with ElA expression plasmids. The results indicate that the G10 stretch located from nucleotide position -239 to -230 and two GC boxes from position -105 to -95 and position -54 to -44 are the primary ElA-responsive elements for repression of the FN gene. Two GC boxes also contain a GIO stretch that is interrupted by the presence of an internal C residue. These sequences overlap with the Spl motif GGGCGG. The ElA gene of human adenovirus type 2 (Ad2) and adenovirus type 5 generates two major species of mRNAs with sizes of 13 and 12S that encode proteins of 289 and 243 amino acid residues (289R and 243R). These proteins have identical N-and C-terminal sequences but differ by the presence of an internal 46-amino-acid sequence in the 289R protein. The domain for transactivation of a variety of viral and cellular promoters resides in this internal sequence specific to the 289R protein (2), and the domain for repression of the enhancer-dependent promoters (4,18,46) resides in the first exon common to these proteins. The ElA gene has the ability to transform primary rodent cells in collaboration with a second oncogene (38,53). This transforming function may be closely related to its functions for immortalizing primary cells (11, 23) and inducing cellular DNA synthesis in quiescent cells (1,28,36,43 ElA expression (20). This is in contrast to mouse embryo-derived AKR-2B cells (3), NIH 3T3 cells (40), and the human fibrosarcoma cell line HT-1080 (13), in which expression levels of FN, its receptor, and actin increased coordinately shortly after growth stimulation of quiescent cells by serum. FN is a large glycoprotein of the extracellular matrix which plays a central role in cell anchorage through cell-extracellular matrix interaction (24, 39, 51) and connects intracellular actin filaments to the extracellular matrix by binding to its receptor (7,25). The fact that FN expression is required for cell proliferation is somewhat paradoxical. Cell adhesion to the substratum seems to be required in order for quiescent cells to exit from the Go phase, while the loosening of cell adhesion is required for cell cycle progression transiently stimulated by extracellular proteases (8,45) or by growth factors (21,22). The latter process is mediated by the structural modification of focal contacts via intracellular mediators. The level of FN expression is greatly reduced in v-src-transformed cells (15) and in primary rat kidney cells transformed by the EJ Ha-ras and the ElA genes (26).In the present study, the ElA-responsive elements for repression of FN gene transcription in rat 3Y1 cells were invest...

show abstract

mentioning

confidence: 71%

Section: Methodsmentioning

confidence: 99%

E1A-responsive elements for repression of rat fibronectin gene transcription.

Nakajima¹,

Nakamura²,

Tsunoda³

et al. 1992

Mol. Cell. Biol.

View full text Add to dashboard Cite

show abstract

“…The addition of the Ca2+ ionophore A23187 had little or no effect on cyr6l expression (Fig. 3, lane 15 (1,52). The cyr6l mRNA appears to belong to a separate category.…”

Section: Methodsmentioning

confidence: 92%

“…Another group of immediate-early genes encodes cytoskeletal and extracellular matrix proteins, including actin (18), fibronectin, the fibronectin receptor, and a-tropomyosin (52). A feature of these genes is that their mRNAs have relatively long half-lives, ranging from 4 to 8 h or more (1,52).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Expression of cyr61, a growth factor-inducible immediate-early gene.

O’Brien¹,

Yang²,

Sanders³

et al. 1990

Mol. Cell. Biol.

287

233

View full text Add to dashboard Cite

A set of immediate-early genes that are rapidly activated by serum or purified platelet-derived growth factor in mouse 3T3 fibroblasts has been previously identified. Among these genes, several are related to known or putative transcription factors and growth factors, supporting the notion that some of these genes encode regulatory molecules important to cel growth. We show here that a member of this set of genes, cyr6l (originally identified by its cDNA 3CH61), encodes a 379-amino-acid polypeptide rich in cysteine residues. cyr61 can be induced through protein kinase C-dependent and -independent pathways. Unlike many immediate-early genes that are transiently expressed, the cyr6l mRNA is accumulated from the G/G1 transition through mid-Gl. This expression pattern is due to persistent transcription, while the mRNA is rapidly turned over during the GJ/G1 transition and in mid-G1 at the same rate. In logarithmically growing cels, the cyr61 mRNA level is constant throughout the cel cycle. Cyr6l contains an N-terminal secretory signal sequence; however, it is not detected in the culture medium by immunoprecipitation. Cyr6l is synthesized maximaly at 1 to 2 h after serum stimulation and has a short half-life within the cell.

show abstract

Cytoarchitecture and cell growth control

Slack¹,

Higgins²

1996

Cell Motil. Cytoskeleton

View full text Add to dashboard Cite

Coordinate induction of fibronectin, fibronectin receptor, tropomyosin, and actin genes in serum-stimulated fibroblasts

Cited by 69 publications

References 36 publications

E1A-responsive elements for repression of rat fibronectin gene transcription.

E1A-responsive elements for repression of rat fibronectin gene transcription.

Expression of cyr61, a growth factor-inducible immediate-early gene.

Cytoarchitecture and cell growth control

Contact Info

Product

Resources

About