Coordinate expression of X and Y haptens during murine embryogenesis

Fenderson, Bruce A.; Holmes, Eric H.; Fukushi, Yasuo; Hakomori, Sen‐itiroh

doi:10.1016/0012-1606(86)90379-9

Cited by 92 publications

(50 citation statements)

References 38 publications

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“…It is present on the blastocyst surface (Fenderson et al 1986, Kimber 1990) as well as on the luminal epithelium of the mouse endometrium (Kimber et al 1988, Kimber & Lindenberg 1990, Zhu et al 1995. Figure 2 Representative micrographs of immunolocalization of FUT4 protein in human endometrium during the menstrual cycle.…”

Section: Discussionmentioning

confidence: 99%

Expression and regulation of fucosyltransferase 4 in human endometrium

Ponnampalam¹,

Rogers²

2008

Reproduction

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It has been suggested that selectin ligands expressed by the endometrial epithelium are essential for the initial adhesion of the blastocyst to the luminal epithelium of human endometrium. One of the enzymes responsible for the production of selectin ligands is fucosyltransferase 4 (FUT4), a member of a1,3 fucosyltransferases. The aims of the present study were to characterize FUT4 mRNA and protein in human endometrium during the menstrual cycle and to investigate the hormonal regulation of FUT4 whose mRNA expression was quantified by real-time PCR in fresh endometrial tissue from cycling women and protein expression was analyzed by immunohistochemistry and Western blotting. Hormonal regulation of FUT4 transcription was investigated using an endometrial explant system. FUT4 mRNA was significantly upregulated in fresh tissues during early and mid-secretory phases when compared with other phases of the menstrual cycle. FUT4 protein was localized to glandular and luminal epithelium and the expression levels followed the same pattern as for FUT4 mRNA. Our data also show that, in proliferative explants, progesterone significantly increased FUT4 transcription and translation after 24 h in culture. The inductive effect of progesterone on FUT4 transcription was lost after 48 h of treatment. Estrogen did not have any significant effects. These data suggest that the upregulation of selectin ligands in the human endometrium at the time of implantation may be mediated, at least in part, by the regulation of FUT4 expression.

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Section: Discussionmentioning

confidence: 99%

Expression and regulation of fucosyltransferase 4 in human endometrium

Ponnampalam¹,

Rogers²

2008

Reproduction

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“…These patterns are discernible first on the surfaces of preimplantation and early postimplantation embryomc cell aggregates (Bird and Kimber 1984;Fenderson et al 1984;Pennington et al 1985;Fenderson et al 1986;Kimber 1986). In the mature organism, expression of distinct oligosaccharide moieties ultimately becomes restricted to specific cell types (Szulman 1960(Szulman , 1962Watkins 1980;Dodd and Jessel 1985).…”

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confidence: 99%

A cloned human cDNA determines expression of a mouse stage-specific embryonic antigen and the Lewis blood group alpha(1,3/1,4)fucosyltransferase.

Kukowska-Latallo¹,

Larsen²,

Nair³

et al. 1990

Genes Dev.

491

274

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The stage-specific embryonic antigen SSEA-I is a cell-surface oligosaccharide molecule expressed with temporal precision during the murine preimplantation period and implicated in adhesive events involving the process of compaction. We used a mammalian transient expression system to isolate a cloned human cDNA that determines expression of the SSEA-I molecule. The cDNA sequence predicts a type II transmembrane protein with a domain structure similar to mammalian glycosyltransferases, but without primary sequence similarity to these enzymes. The carboxy-terminal domain of this protein was shown to be catalytically active as a fucosyltransferase when expressed in COS-I cells as a portion of a secreted protein A fusion peptide. The enzyme is an exceptional glycosyltransferase in that it can use both type I and type II oligosaccharides as acceptor substrates to generate subterminal Fucc~(l,4)-and Fuc~(l,3)-linkages, respectively, in a manner analogous to the human Lewis blood group fucosyltransferase. Southern blot analysis shows that the cDNA corresponds to sequences syntenic to the Lewis locus on chromosome 19. These results indicate that this cDNA is the product of the human Lewis blood group locus, provide genetic confirmation of the hypothesis that this enzyme can catalyze two distinct transglycosylation reactions, and outline an approach to the isolation of other sequences that determine expression of developmentally regulated oligosaccharide antigens.

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“…F9 cells contain many surface carbohydrate antigens that are shared with those in early mouse embryos (17)(18)(19)(20)(21)(22). Surface glycoconjugates in F9 cells contain the repeating disaccharide [3Gal␤1-4GlcNAc␤1] n or poly-N-acetyllactosamine sequence (23)(24)(25) and the stage-specific embryonic antigen SSEA-1 (also known as the Lewis X antigen or Le X ), which has the structure Gal␤1-4(Fuc␣1-3)GlcNAc␤1-R (17,26).…”

mentioning

confidence: 99%

Transcriptional Regulation of α1,3-Galactosyltransferase in Embryonal Carcinoma Cells by Retinoic Acid

Cho

Yeh

Cho

et al. 1996

Journal of Biological Chemistry

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Treatment of mouse teratocarcinoma F9 cells with alltrans-retinoic acid (RA) causes a 9-fold increase in steady-state levels of mRNA for UDP-Gal:␤-D-Gal ␣1,3-galactosyltransferase (␣1,3GT) beginning at 36 h. Enzyme activity rises in a similar fashion, which also parallels the induction of laminin and type IV collagen. Nuclear run-on assays indicate that this increase in ␣1,3GT in RA-treated F9 cells, like that of type IV collagen, is transcriptionally regulated. Differentiation also results in increased secretion of soluble ␣1,3GT activity into the growth media. The major ␣-galactosylated glycoprotein present in the media of RA-treated F9 cells, but not of untreated cells, was identified as laminin. Differentiation of F9 cells is accompanied by an increase in ␣-galactosylation of membrane glycoproteins and a decrease in expression of the stage-specific embryonic antigen, SSEA-1 (also known as the Lewis X antigen or Le X ), which has the structure Gal␤1-4(Fuc␣1-3)GlcNAc␤1-R. However, flow cytometric analyses with specific antibodies and lectins, following treatment of cells with ␣-galactosidase, demonstrate that differentiated cells contain Le X antigens that are masked by ␣-galactosylation. Thus, RA induces ␣1,3GT at the transcriptional level, resulting in major alterations in the surface phenotype of the cells and masking of Le X antigens.

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Coordinate expression of X and Y haptens during murine embryogenesis

Cited by 92 publications

References 38 publications

Expression and regulation of fucosyltransferase 4 in human endometrium

Expression and regulation of fucosyltransferase 4 in human endometrium

A cloned human cDNA determines expression of a mouse stage-specific embryonic antigen and the Lewis blood group alpha(1,3/1,4)fucosyltransferase.

Transcriptional Regulation of α1,3-Galactosyltransferase in Embryonal Carcinoma Cells by Retinoic Acid

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