Cooperative regulation of PBI1 and MAPKs controls WRKY45 transcription factor in rice immunity

Ichimaru, Kota; Yamaguchi, Koji; Harada, Kenichi; Nishio, Y.; Hori, Momoka; Ishikawa, Kazuya; Inoue, Haruhiko; Shigeta, Shusuke; Inoue, Kento; Shimada, Keita; Yoshimura, Satomi; Takeda, Takumi; Yamashita, Eiki; Fujiwara, Toshimichi; Nakagawa, Atsushi; Kojima, Chojiro; Kawasaki, Tsutomu

doi:10.1038/s41467-022-30131-y

Cited by 31 publications

(21 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nevertheless, adverse abiotic stresses such as salt, drought and cold severely limit crop growth, development and yield ( Corrales et al., 2017 ; Meng et al., 2023 ). Genetic engineering is widely proven to be a powerful way to improve crop resistance, among them, transcription factors (TFs) are promising and efficient members to solve the problems encountered by plants in adversity, such as AP2/ERF, NAC, WRKY, MYB, GRAS and Dof TF families ( Gupta et al., 2015 ; Erpen et al., 2018 ; Ichimaru et al., 2022 ; Zhang et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide survey and expression analysis of Dof transcription factor family in sweetpotato shed light on their promising functions in stress tolerance

Zhang

Dong

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

DNA-binding with one finger (Dof) transcription factors play a crucial role in plant abiotic stress regulatory networks, although massive Dofs have been systematically characterized in plants, they have not been identified in the hexaploid crop sweetpotato. Herein, 43 IbDof genes were detected to be disproportionally dispersed across 14 of the 15 chromosomes of sweetpotato, and segmental duplications were discovered to be the major driving force for the expansion of IbDofs. The collinearity analysis of IbDofs with their related orthologs from eight plants revealed the potential evolutionary history of Dof gene family. Phylogenetic analysis displayed that IbDof proteins were assigned into nine subfamilies, and the regularity of gene structures and conserved motifs was consistent with the subgroup classification. Additionally, five chosen IbDof genes were shown to be substantially and variably induced under various abiotic conditions (salt, drought, heat, and cold), as well as hormone treatments (ABA and SA), according to their transcriptome data and qRT-PCR experiments. Consistently, the promoters of IbDofs contained a number of cis-acting elements associated with hormone and stress responses. Besides, it was noted that IbDof2 had transactivation activity in yeasts, while IbDof-11/-16/-36 did not, and protein interaction network analysis and yeast two-hybrid experiments revealed a complicated interaction connection amongst IbDofs. Collectively, these data lay a foundation for further functional explorations of IbDof genes, especially with regards to the possible application of multiple IbDof members in breeding the tolerant plants.

show abstract

Section: Introductionmentioning

confidence: 99%

Genome-wide survey and expression analysis of Dof transcription factor family in sweetpotato shed light on their promising functions in stress tolerance

Zhang

Dong

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Based on the MALDI-TOF-MS analysis of the synthetic products (Figure ), the products from combinations (1) and (4) with AtChiC-G75W/D114N, from combinations (1), (2), and (3) with AtChiC-G75W/W324F, and from combination (4) with BcChiA1-W433A exhibited sufficient purity of (GlcNAc) 7 to be employed for the chitin-induced disease-resistant experiments in plants. , Among these reaction systems, combination (1) with AtChiC-G75W/D114N, combination (1) with AtChiC-G75W/W324F, and combination (4) with BcChiA1-W433A gave the synthetic products in higher yields (Table ). Considering the higher yield of (GlcNAc) 2 -oxa in obtaining oxazoline derivatives (Table ), the reaction systems using (GlcNAc) 2 -oxa/(GlcNAc) 5 and AtChiC-G75W/D114N or AtChiC-G75W/W324F are more advantageous for obtaining (GlcNAc) 7 with sufficient quality.…”

Section: Discussionmentioning

confidence: 99%

“…(GlcNAc) 7 was reported to have higher elicitor activity , and has been often used in the studies on chitin-induced disease resistance in plants. , This situation prompted us to efficiently produce (GlcNAc) 7 using the oxazoline derivatives. We first synthesized sugar oxazolines of (GlcNAc) n , (GlcNAc) n -oxa, using the method reported previously, and the binding affinities of (GlcNAc) n -oxa toward a plant chitinase mutant were determined by isothermal titration calorimetry (ITC).…”

Section: Introductionmentioning

confidence: 99%

Plant Chitinase Mutants as the Catalysts for Chitooligosaccharide Synthesis Using the Sugar Oxazoline Derivatives

Umemoto

Saito

Noguchi

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

Sugar oxazolines, (GlcNAc) n -oxa (n = 2, 3, 4, and 5), were synthesized from a mixture of chitooligosaccharides, (GlcNAc) n (n = 2, 3, 4, and 5), and utilized for synthesis of (GlcNAc) 7 with higher elicitor activity using plant chitinase mutants as the catalysts. From isothermal titration calorimetry, the binding affinity of (GlcNAc) 2 -oxa toward an inactive mutant obtained from Arabidopsis thaliana GH18 chitinase was found to be higher than those of the other (GlcNAc) n -oxa (n = 3, 4, and 5). To synthesize (GlcNAc) 7 , the donor/acceptor substrates with different size combinations, (GlcNAc) 2 -oxa/(GlcNAc) 5 ( 1), (GlcNAc) 3 -oxa/ (GlcNAc) 4 (2), (GlcNAc) 4 -oxa/(GlcNAc) 3 (3), and (GlcNAc) 5 -oxa/(GlcNAc) 2 (4), were incubated with hypertransglycosylating mutants of GH18 chitinases from A. thaliana and Cycas revoluta. The synthetic activities of these plant chitinase mutants were lower than that of a mutant of Bacillus circulans chitinase A1. Nevertheless, in the plant chitinase mutants, the synthetic efficiency of combination (1) was higher than those of the other combinations (2), (3), and (4), suggesting that the synthetic reaction is mostly dominated by the binding affinities of (GlcNAc) n -oxa. In contrast, the Bacillus enzyme mutant with a different subsite arrangement synthesized (GlcNAc) 7 from combination (1) in the lowest efficiency. Donor/acceptor-size dependency of the enzymatic synthesis appeared to be strongly related to the subsite arrangement of the enzyme used as the catalyst. The A. thaliana chitinase mutant was found to be useful when combination (1) is employed for the substrates.

show abstract

“…Among them, WRKY and bZIP (basic leucine zipper) are involved in the SA-dependent activation of PR genes. WRKY are one of the TFs families that play important roles in immune responses 39 . For example, WRKY has been reported to negatively regulate the basal resistance of A. thaliana against pathogenic Pseudomonas syringae 40 .…”

Section: Transcriptomic Reprogramming In Rice Leavesmentioning

confidence: 99%

Engineering Climate-Resilient Rice Using a Nanobiostimulant-based “Stress Training” Strategy

Chen

Pan

Zhao

et al. 2023

Preprint

View full text Add to dashboard Cite

Under a changing climate, cultivating climate-resilient crops will be critical to maintaining food security. Here, we propose the application of ROS-generating nanoparticles as nanobiostimulants to trigger stress/immune responses, and subsequently increase the stress resilience of plants. We established three regimens of AgNPs-based “stress training”: seed priming (SP), leaf priming (LP), and combined seed- and leaf- priming (SLP). Trained rice seedlings were then exposed to either rice blast fungus (M. oryzae.) or chilling stress (10 ºC). The results show that all “stress training” regimes, particularly SLP significantly enhanced the resistance of rice against the fungal pathogen (lesion size reduced by 82% relative to un-trained control). SLP training also significantly enhanced rice tolerance to cold stress. Under cold conditions, SLP training significantly increased leaf biomass by 35% compared to controls. The mechanisms for the enhanced resilience were investigated with metabolomic and transcriptomic profiling, which show that “stress training” induced considerable metabolic and transcriptional reprogramming in rice leaves. AgNPs-boosted ROS activated stress signaling pathways by oxidative post-translational modifications of stress related kinases, hormones, and transcriptional factors (TFs). These signaling pathways subsequently modulated the expression of defense genes, including specialized metabolites (SMs) biosynthesis genes, cell membrane lipid metabolism genes, and pathogen-plant interaction genes. These AgNPs-triggered metabolic and transcriptional reprogramming enable rice plants to mount a more rapid and intense response to future stresses. This nanobiostimulant-based strategy for increasing the stress resilience of crops will increase yield vigor against a changing climate and will contribute to sustainable agriculture by reducing agrochemical use.

show abstract

Cooperative regulation of PBI1 and MAPKs controls WRKY45 transcription factor in rice immunity

Cited by 31 publications

References 68 publications

Genome-wide survey and expression analysis of Dof transcription factor family in sweetpotato shed light on their promising functions in stress tolerance

Genome-wide survey and expression analysis of Dof transcription factor family in sweetpotato shed light on their promising functions in stress tolerance

Plant Chitinase Mutants as the Catalysts for Chitooligosaccharide Synthesis Using the Sugar Oxazoline Derivatives

Engineering Climate-Resilient Rice Using a Nanobiostimulant-based “Stress Training” Strategy

Contact Info

Product

Resources

About