Cooperation of Hepatocytes in vitro in the Rhythm of Protein Synthesis is Promoted by the GM1 Ganglioside Contained in Vesicles and Liposomes

Brodsky, V. Ya.; Nechaeva, N. V.; Zvezdina, N. D.; Novikova, T. E.; Gvazava, I. G.; Fateeva, V. I.; Мальченко, Л. А.

doi:10.1023/b:bibu.0000007708.87438.60

Biology Bulletin

2003

DOI: 10.1023/b:bibu.0000007708.87438.60

|View full text |Cite

Cooperation of Hepatocytes in vitro in the Rhythm of Protein Synthesis is Promoted by the GM1 Ganglioside Contained in Vesicles and Liposomes

V. Ya. Brodsky

N. V. Nechaeva

N. D. Zvezdina

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2005

2022

Publication Types

Select...

Article3

Relationship

Self Cite1

Independent2

Authors

Journals

Cited by 3 publications

(1 citation statement)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We used the α 1 -adrenomimetic phenylephrine at a dose inducing oscillations and increase of Ca 2+ concentration in the cytoplasm. After the treatment of sparse cultures of hepatocytes with phenylephrine, a protein rhythm synthesis was found in the, which was not found in control cultures (Brodsky et al , 2003a). It was shown that the chelator of intracellular calcium BAPTA-AM eliminates the rhythm in dense, normally synchronous cultures (Zvezdina et al , 2003).…”

mentioning

confidence: 99%

Age-related features of protein synthesis rhythm. Effects of extracellular medium

et al. 2005

Self Cite

View full text Add to dashboard Cite

Circahoralian, or ultradian, intracellular rhythms, including protein synthesis rhythm, were described in various cells both in situ and in vitro (for reviews see Brodsky, 1975; Ultradian Rhythms …, 1992). They have been studied for a long time in the cultures of hepatocytes as well. Visualization of any rhythm in a cell culture is only possible when oscillations are synchronized due to intercellular cooperation. The rhythm expression depends on the degree of synchrony, i.e., on cooperative activity of cell in the formation of an aggregate rhythm of a cell population. The amplitude of rhythm, or range of oscillations, is a visible reflection of this cooperation. In the case when each individual cell has a rhythm, but oscillations in different cells are antiphasic, the aggregate kinetics of a population will not be oscillatory. When the phases of oscillations correspond to each other, a rhythm is visualized and, the closer the phases of oscillations of individual cells, the higher the amplitude of the aggregate rhythm.We have already shown that the amplitude of oscillations of protein synthesis intensity in the cultures of hepatocytes from old rats is lower than that from young animals (Brodsky et al. , 2001), which characterized a low cooperative activity of the cells of old animals. However, these data were preliminary: we assayed the kinetics of protein synthesis only in two old rats. In order to confirm the first observations, it was necessary to carry out experiments of a larger material and, in case of a positive result, clarify possible causes of agerelated changes and establish to what extent they are reversible.When studying intercellular cooperation, we investigated dense, fast self-synchronizing in a fresh medium, hepatocytes and sparse asynchronous cultures (Brodsky et al. , 1994(Brodsky et al. , , 1996a. We used the synchronizers we found earlier: gangliosides (Brodsky et al. , 1996a(Brodsky et al. , , 1997Zvezdina et al. , 2000). Gangliosides are cell membrane glycolipids; they are constantly secreted in extracellular medium and incorporated in other cells and affect many intracellular processes (for reviews see Hakomori, 1981Hakomori, , 2000Tettamanti and Riboni, 1994;Yates and Rampersaud, 1998). In our experiments, asynchronous sparse cultures of hepatocytes were synchronized soon after addition of a standard mix of bovine brain gangliosides (BBG: 20% GM1, 40% GD1a, 16% GD1b, and ca. 4% of others) or individual fractions (GM1 or GD1a). The synchronizing effect of gangliosides can be reproduced by a medium conditioned by dense cultures (Brodsky et al. , 1995(Brodsky et al. , , 2000b.In order to simulate the influence of gangliosides on intracellular processes, we studied their well known effect on the concentration of calcium ions in the cytoAbstract -Cell interactions have been studied in cultures pf hepatocytes from young and old rats. The rhythm of protein synthesis is an index of cell interaction and synchronization in culture, while the amplitude of oscillations characterized cell cooperation i...

show abstract

mentioning

confidence: 99%

Age-related features of protein synthesis rhythm. Effects of extracellular medium

et al. 2005

Self Cite

View full text Add to dashboard Cite

show abstract

Direct cell-cell communications and social behavior of cells in mammals, protists, and bacteria. Possible causes of multicellularity

Brodsky

2009

Russ J Dev Biol

View full text Add to dashboard Cite

Gangliosides in Orchestration of Intercellular Communication, Development, Neuronal Pathology and Carcinogenesis

Brodsky

2022

Russ J Dev Biol

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Cooperation of Hepatocytes in vitro in the Rhythm of Protein Synthesis is Promoted by the GM1 Ganglioside Contained in Vesicles and Liposomes

Cited by 3 publications

References 14 publications

Age-related features of protein synthesis rhythm. Effects of extracellular medium

Age-related features of protein synthesis rhythm. Effects of extracellular medium

Direct cell-cell communications and social behavior of cells in mammals, protists, and bacteria. Possible causes of multicellularity

Gangliosides in Orchestration of Intercellular Communication, Development, Neuronal Pathology and Carcinogenesis

Contact Info

Product

Resources

About