Cooperation behavior of fore‐ And hindlimbs during jumping in <i>Rana dybowskii</i> and <i>Xenopus laevis</i>

Li, Mo; Gao, Zibo; Wang, Jili; Wei, Song; Zhang, Qingzhu; Jin, Tao; Ren, Lili

doi:10.1002/ece3.7589

Cited by 4 publications

(2 citation statements)

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“…For example, metatarsals develop at a faster rate than metacarpals ( Fig. 4C ), which aligns with functional considerations 37 . Together, based on our micro-CT data, one can support previously noted observations or make new predictions for further experimental testing of (long) bone growth.…”

Section: Introductionsupporting

confidence: 78%

“…Importantly, this difference was not attributed to absolute bone measurements but rather in relation to the animal's overall length (data not shown). This phenomenon may be linked to the frogs' utilization of their hind limbs for swimming, whereas even their forelimbs are primarily employed for food handling 36,37 . Moreover, the length of the tarsal bone such as astragalus and calcaneum appears to be less critical for escape or startle responses, such as swim kicking, compared to the lengths of the femur and tibiofibular, which are correlated with the muscle mass of the thigh and the calf.…”

Section: Micro-ct Data Analysis Of Long Bone Growth Dynamicsmentioning

confidence: 99%

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Unveiling Vertebrate Development Dynamics in FrogXenopus laevisusing Micro-CT Imaging

Jakub,

Michaela,

Alice

et al. 2024

Preprint

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BackgroundXenopus laevis, the African clawed frog, is a versatile vertebrate model organism employed across various biological disciplines, prominently in developmental biology to elucidate the intricate processes underpinning body plan reorganization during metamorphosis. Despite its widespread utility, a notable gap exists in the availability of comprehensive datasets encompassingXenopus’late developmental stages.FindingsIn the present study, we harnessed micro-computed tomography (micro-CT), a non-invasive 3D imaging technique utilizing X-rays to examine structures at a micrometer scale, to investigate the developmental dynamics and morphological changes of this crucial vertebrate model. Our approach involved generating high-resolution images and computed 3D models of developingXenopusspecimens, spanning from premetamorphosis tadpoles to fully mature adult frogs. This extensive dataset enhances our understanding of vertebrate development and is adaptable for various analyses. For instance, we conducted a thorough examination, analyzing body size, shape, and morphological features, with a specific emphasis on skeletogenesis, teeth, and organs like the brain at different stages. Our analysis yielded valuable insights into the morphological changes and structure dynamics in 3D space duringXenopus’development, some of which were not previously documented in such meticulous detail. This implies that our datasets effectively capture and thoroughly examineXenopusspecimens. Thus, these datasets hold the solid potential for additional morphological and morphometric analyses, including individual segmentation of both hard and soft tissue elements withinXenopus.ConclusionsOur repository of micro-CT scans represents a significant resource that can enhance our understanding ofXenopus’development and the associated morphological changes. The widespread utility of this amphibian species, coupled with the exceptional quality of our scans, which encompass a comprehensive series of developmental stages, opens up extensive opportunities for their broader research application. Moreover, these scans have the potential for use in virtual reality, 3D printing, and educational contexts, further expanding their value and impact.Graphical abstract & lay summary3D images of selected developmental stages ofX. laevisin a comparison (scale bar = 10 mm).Lay summaryX-ray tomography was used to examine the African clawed frog (Xenopus laevis). An extensive data set of specimens from tadpoles to adult frogs provides novel insights into the changes and developmental dynamics of selected structures, which opens avenues to an improved understanding of this crucial animal model.

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Section: Introductionsupporting

confidence: 78%

Section: Micro-ct Data Analysis Of Long Bone Growth Dynamicsmentioning

confidence: 99%