CoolClot, a novel hemostatic agent for controlling life-threatening arterial bleeding

Abstract

BACKGROUND:Uncontrolled bleeding is the first leading cause of preventable death in the battlefield and the 2nd cause of mortality in civil accidents. Incompressible hemorrhage control is among the interventions that drastically increase the survival rate in wound individuals. We have previously shown that a certain mixture of bentonite and zeolite minerals can significantly decrease the bleeding in rats.METHODS:In this study, nine healthy hybrid dogs were selected and after induction of anesthesia with ether, either arterial puncture by a needle or arteriotomy was performed on both groin regions of the dogs. For control arteries (either the right or left femoral artery), only pressure by sterilized gauze was performed, while for the femoral arteries of the opposite side, our invented hemostatic agent, namely CoolClot, was topically used before applying the pressure. In the second stage of the study, to assess the coagulation time, blood samples were collected from 10 volunteer students.RESULTS:CoolClot significantly decreased the bleeding time in animals whose femoral arteries were cut or punctured. In the human phase of the study, the mean coagulation time in control blood samples was 253.4±44.1 seconds, whereas it was 149.5±50.0, 162.3±74.6 and 143.4±114.6 seconds, respectively in blood samples treated with bentonite, zeolite and CoolClot (P<0.05).CONCLUSIONS:As controlling bleeding after a life-threatening arterial damage is critical for increasing the chance of survival, the results obtained in this study indicate the significant efficacy of CoolClot in shortening the bleeding time. Our experiments also indicate that CoolClot can significantly reduce the clotting time in human blood samples.

Morphological, cytotoxicity, and coagulation assessments of perlite as a new hemostatic biomaterial

“…The lowest coagulation time of about 140 ± 2 seconds was obtained for pearlite with a powder concentration of 50 mg mL −1 , while this time was longer for other powders (control: 315 ± 2 seconds, kaolin: 192 ± 5 seconds, and bentonite: 202 ± 4 seconds for 50 mg mL −1 ). Mortazavi et al , 40 showed that the mean coagulation time in control blood samples was 253.4 seconds, whereas it was 149.5, 162.3and 143.4 seconds, respectively in blood samples treated with bentonite, zeolite, and CoolClot (bentonite–zeolite minerals).…”

“… 39 Mortazavi et al showed that a specific mixture of bentonite–zeolite minerals has a significant effect in shortening the bleeding time and can also significantly reduce the blood clotting time. 40 Kaolin is prepared as a bioceramic from the family of layered aluminosilicates [Al 4 (Si 4 O 10 )(OH) 8 ] from the weathering of the feldspar mineral group. 25 Kaolin, an aluminum phyllosilicate clay mineral, is a potent cation-based activator of the intrinsic pathway of coagulation.…”

The Effect of Chitohem Bleeding Inhibitor Powder on Pain and Bleeding After Tonsillectomy by Suturing Method

“…Wedmore et al 17 also showed that Celox was successful in all cases of wounds where gauze packing has failed, and homeostasis was established wherever physicians could clearly see the dressing. The results of a study by Mortazavi et al 18 showed the effect of the Cool Clot coagulation factor in reducing the time required to stop bleeding in the animal model of dogs and reducing the time of clot formation in human blood samples. Nouri et al 19 stated that calcium sulphate and ferric sulphate could control hepatic hemorrhage and are effective hemostatic agents in controlling hepatic hemorrhage in the animal model.…”

Exploring Rajaswala Paricharya: A Holistic Ayurvedic Perspective on Women's Well-being

“…Intense exercise has been associated with luteal phase defects and menstrual dysfunctions, with athletes engaging in vigorous exercise more likely to experience oligomenorrhea and amenorrhea. Conversely, moderate exercise is slightly associated with longer menstrual periods [32] .…”