Cool-night temperature induces spike emergence and affects photosynthetic efficiency and metabolizable carbohydrate and organic acid pools in Phalaenopsis aphrodite

Chen, Wen-Huei; Tseng, Ya-Chen; Liu, Yo-Ching; Chuo, Chuo-Min; Chen, Pai-Ting; Tseng, Kai-Meng; Yeh, Yi-Chun; Ger, Mang‐Jye; Wang, Heng-Long

doi:10.1007/s00299-008-0591-0

Cited by 52 publications

(63 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Leaf discs (ca. 0.28 cm 2 ) were cut from the middle and approximately 0.5 cm away from the main vein of the second leaf with a cork borer (Chen et al 2008). After all leaves were completely removed, a knife was used to harvest the stem from the junction between the base of the flower stalk bud and the shortened stem, and the flower stalk was removed .…”

Section: Methodsmentioning

confidence: 99%

“…To determine soluble sugar concentrations, leaf discs and stems were separately homogenized and extracted with 80% (v/v) ethanol at 80°C (Chen et al 2008). Sugars were separated on a CarboPac PA10 column (Dionex Corporation, Sunnyvale, CA, USA) with 18 mM NaOH as an eluent and quantified by use of a Dionex pulsed amperometric detector (Chen et al 2008).…”

Section: Metabolite Analysesmentioning

confidence: 99%

“…Organic acids were analyzed by anion exchange chromatography (AS-11, Dionex Corporation, Sunnyvale, CA, USA) using 5-100 mM NaOH for linear gradient elution and the eluted acids were quantified using a Dionex electrochemical detector (ED50, Dionex Corporation, Sunnyvale, CA, USA ) (Chen et al 2008). The contents of organic acids (malate and citrate) were calculated from the integrated peak area of the corresponding standards, total organic acid [μg g [g].…”

Section: Metabolite Analysesmentioning

confidence: 99%

“…Sugars were separated on a CarboPac PA10 column (Dionex Corporation, Sunnyvale, CA, USA) with 18 mM NaOH as an eluent and quantified by use of a Dionex pulsed amperometric detector (Chen et al 2008). The contents of soluble sugars (glucose, fructose and sucrose) were calculated from the integrated peak area of the corresponding standards, total soluble sugar [μg g [g].…”

Section: Metabolite Analysesmentioning

confidence: 99%

“…Phalaenopsis is an obligate CAM plant with a day-night fluctuation of photosynthetic characteristics: malic acid accumulation with open stomata during the night time and starch deposition with closed stomata during the day time, which implies that the maximum malate and starch contents should occur at dawn and dusk, respectively. On the contrary, the minimum malate and starch contents are exhibited at dusk and dawn, respectively (Endo and Ikusima 1989, Guo and Lee 2006, Chen et al 2008, Pollet et al 2011. Hence, we examined the diurnal changes in photosynthetic capacity of Phalaenopsis exposed to lowering [20 μmol(photon) m ] irradiances, together with the daynight changes of the contents of photosynthesis-related metabolites in the new and fully expanded leaf and stem to clarify how limiting light affects the day-night variations in photosynthetic performance, and the cause-and-effect of photosynthates on the spike development of Phalaenopsis.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Effect of low irradiance on the photosynthetic performance and spiking of Phalaenopsis

Liu¹,

Liu²,

Lin³

et al. 2016

Photosynt.

View full text Add to dashboard Cite

Lowering irradiance can delay the flower stalk, i.e., spike development, in order to schedule flowering time of Phalaenopsis; however, the effect on photosynthetic performance and spiking inhibition remains poorly understood. We compared light and shade treatments of Phalaenopsis aphrodite subsp. formosana in order to determine how limiting light affects day-night changes in the photosynthetic capacity of leaves and the carbon pool of leaves and stems resulting in delayed spiking. The low irradiance treatment [20 μmol(photon) m ] for six weeks did not affect potential functions of photosynthetic apparatus estimated by chlorophyll a fluorescence analysis, but it significantly reduced the net CO 2 uptake and O 2 evolution rates, carbohydrate and organic acid concentrations, and amplitudes of CAM activity in new and fully expanded leaves of Phalaenopsis and delayed the spiking compared with the control kept at 150 μmol(photon) m 2 s 1. The shortened stem contained a remarkably high sucrose concentration, accounting for more than 80% of total soluble sugars for both treatments throughout the day. Moreover, the sucrose concentration was unaffected by the lowering of irradiance. The relationship between the sucrose content and spiking seemed to be loose; the major factor(s) for spiking in Phalaenopsis remained to be ascertained as the flower stalk bud is attached to the shortened stem.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Metabolite Analysesmentioning

confidence: 99%

Section: Metabolite Analysesmentioning

confidence: 99%

Section: Metabolite Analysesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effect of low irradiance on the photosynthetic performance and spiking of Phalaenopsis

Liu¹,

Liu²,

Lin³

et al. 2016

Photosynt.

View full text Add to dashboard Cite

show abstract

Exploration of Sweet Immunity to Enhance Abiotic Stress Tolerance in Plants: Lessons from CAM

2016

View full text Add to dashboard Cite

The concept of 'sweet immunity' or 'sugar-enhanced defence' is based on the accumulating evidence that sweet, endogenous saccharides might act as signalling molecules that are activated by exposure to stress and hence initiate signal amplification and lead to more rapid and robust activation of defence, immunity and stress tolerance. Sugars such as glucose, fructose and sucrose have acquired important regulatory functions in evolution and are becoming more and more recognized as signalling molecules in plants controlling gene expression related to plant metabolism, stress resistance and development. This offers opportunities for 'sweet priming', defined as a physiological process that prepares plants for a faster and/or stronger defence response to future stress conditions, but does not impose the costs associated with full implementation of an induced defence

show abstract

Low night temperature acclimation of Phalaenopsis

et al. 2011

View full text Add to dashboard Cite

The capability of Phalaenopsis to acclimate its photosynthetic capacity and metabolic activity to cool night temperature conditions is crucial for improving orchid production in terms of efficient greenhouse heating. The extent to which Phalaenopsis possesses acclimation potential and the mechanistic background of the metabolic processes involved, have, however, not been studied before. Plants were subjected to a direct and gradual shift from a day to night temperature regime of 28/28-28/16°C, the cold stress and cold acclimation treatment, respectively. In comparison with the cold stress treatment, the cold acclimation treatment led to a higher malate accumulation and a reduction in leaf net CO(2) uptake. Consistently, the contribution of respiratory CO(2) recycling to nocturnal malate synthesis was calculated to be 23.5 and 47.0% for the cold stress and cold acclimation treatment, respectively. Moreover, the lower levels of starch measured in the cold acclimated leaves confirmed the suggested enhanced respiratory CO(2) recycling, implying that Phalaenopsis CAM operation evolved towards CAM idling. It is, however, plausible that this adjustment was not an effect of the low night temperature per se but a consequence of cool-root induced drought stress. Apart from that, at the start of the photoperiod, membrane stability showed a depression which was directly counteracted by an increased generation of glucose, fructose and sucrose. From these observations, it can be concluded that the observed plasticity in CAM operation and metabolic flexibility may be recognized as important steps in the low night temperature acclimation of Phalaenopsis.

show abstract

Cool-night temperature induces spike emergence and affects photosynthetic efficiency and metabolizable carbohydrate and organic acid pools in Phalaenopsis aphrodite

Cited by 52 publications

References 48 publications

Effect of low irradiance on the photosynthetic performance and spiking of Phalaenopsis

Effect of low irradiance on the photosynthetic performance and spiking of Phalaenopsis

Exploration of Sweet Immunity to Enhance Abiotic Stress Tolerance in Plants: Lessons from CAM

Low night temperature acclimation of Phalaenopsis

Contact Info

Product

Resources

About