Cooking with EDTA reduces nutritional value of Vicia faba beans

El-Said, Karim Samy; Othman, Samir M.; Mansour, Fotouh R.; Kabil, Doaa Ibrahim; Khairy, Mostafa Hossam

doi:10.1016/j.btre.2019.e00322

Cited by 5 publications

(7 citation statements)

References 21 publications

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“…To accelerate the cooking process and reduce the cost of cooking, some chefs use additives such as citric acid, sodium bicarbonate, and ethylenediaminetetraacetic acid (EDTA) (Lamas et al, 2014; Lanigan and Yamarik, 2002) where the latter was found to decrease the degree of starch gelatinization of black beans ( Phaseolus vulgaris L. ) (Mubaiwa et al, 2017). The amount of EDTA used in beans cooking has been variable and ranged from 0.5 g/L to 2 g/L, depending on the boiling time of beans (El-Naggar et al, 2019b). About 1.5 h were enough for V. faba beans to be well cooked in the presence of 2 g/L of EDTA, compared with 6 h in EDTA-free water.…”

Section: Introductionmentioning

confidence: 99%

“…In previous studies, administration of EDTA orally produced reproductive and developmental adverse effects in animals (Khalil et al, 2008; Lanigan and Yamarik, 2002). According to our recent study, we have reported that adding EDTA to beans during cooking led to the formation of new compounds and ultimately diminishing the nutritional values of beans (El-Naggar et al, 2019b). Therefore, we hypothesized that the presence of EDTA in bean cooking media might be toxic upon administration into naïve mice for prolonged time.…”

Section: Introductionmentioning

confidence: 99%

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Toxicity of bean cooking media containing EDTA in mice

et al. 2020

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The possible renal and hepatic toxicities of ethylenediaminetetraacetic acid (EDTA) in bean cooking media were studied using 100 male albino mice. Two sublethal doses of EDTA were used to explore their toxic effects; 20 mg/kg and 200 mg/kg, which corresponded to 1/100th and 1/10th of LD50, respectively. Accordingly, the toxicity study was performed using 50 mice, divided into five groups ( n = 10/group) as follows: group 1 (Gp1) served as a negative control and was orally administered normal saline; group 2 (Gp2) was administered the bean cooking medium; group 3 (Gp3) was administered EDTA (200 mg/kg); group 4 (Gp4) was administered bean cooking medium containing 20 mg/kg of EDTA; and group 5 (Gp5) was administered bean cooking medium containing 200 mg/kg of EDTA. The results showed no significant changes in liver and kidney functions in Gp2 while Gp3, Gp4, and Gp5 exhibited significant increases in adverse liver and kidney function markers. Hematocrit values were significantly decreased in Gp3 and Gp5, while the total white blood cells counts were significantly decreased in Gp3 and significantly increased in Gp5. The number of platelets was decreased in Gp3, Gp4, and Gp5. The blood levels of sodium (Na+), iron (Fe2+), and calcium (Ca2+) were decreased in Gp3, Gp4, and Gp5 due to the chelating effects of EDTA. The hepatic and renal architectures were disorganized in Gp3, Gp4, and Gp5 with some hemorrhagic manifestations in livers and kidneys of mice. These results demonstrate that EDTA in bean cooking is harmful in mice under the conditions of this study, and the potentially harmful effects in humans supports restricting its use.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Toxicity of bean cooking media containing EDTA in mice

et al. 2020

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“…It is well known that ethylene diamine tetra-acetic acid (EDTA) promotes the depletion of some metals and alters some cellular physiological functions on the living organisms [1,2]. It modifies the enterocytes permeability of cell membranes, which in turn enhances the rate of absorption of some biomolecules and drugs [3].…”

Section: Introductionmentioning

confidence: 99%

Enhancing Antitumor Efficacy of Cisplatin Low Dose by EDTA in Ehrlich Ascetic Carcinoma Bearing Mice

El‐Naggar

El-Said

Mobasher

et al. 2019

Braz. arch. biol. technol.

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In a recent study, the treatment of different human cancer cell lines in vitro with ethylene diamine tetra-acetic acid (EDTA) showed a promising anticancer activity which could be a novel promising approach for cancer treatment. The aim of this study is to address the ability of EDTA to enhance the antitumor efficacy of the low dose of cisplatin (Cis) treatment in Ehrlich ascetic carcinoma (EAC) bearing mice. Sixty female albino mice were divided into six groups. The 1 st group of mice was served as a negative control. 2 nd -6 th groups were inoculated intraperitoneal (i.p) with 2×10 6 EAC cells/mouse. After one day of inoculation, the 2 nd , 3 rd and 4 th groups were injected daily for 6 days (early treatment) with phosphate buffer saline, low dose of Cis and Cis/EDTA, respectively. After six days, the 5 th and 6 th groups were injected with the low dose of Cis and Cis/EDTA for 6 consecutive days (late treatment), respectively. At day 14, all groups of mice were sacrificed, sera were collected for biochemical assessment, HIGHLIGHTS• EDTA enhanced the antitumor efficacy of the low dose of Cisplatin.• Low dose of Cisplatin did not inhibit tumor growth • Co-treatment with EDTA delayed and decreased tumor growth.• EDTA decreased the toxicity of Cisplatin. 2 El-Naggar, S.A.; et al.

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“…Only a small number of studies go beyond the general detectability and investigate for instance stability constants of Cu 2+ ‐, Zn 2+ ‐ and Ni 2+ ‐EDTA by ESI‐MS 22 . Barely any information is available on the fragmentation behavior of uncomplexed EDTA, 23,24 while no data on metal‐EDTA complexes are available.…”

Section: Introductionmentioning

confidence: 99%

Fragmentation behavior of EDTA complexes under different activation conditions

Beck

2021

J Mass Spectrom

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Ethylenediaminetetraacetic acid (EDTA) is an aminopolycarboxylic acid and complexation agent that is able to bind a large variety of metals. The formation of highly stable metal‐EDTA complexes is generally very quick. This has led to the use of EDTA in a variety of applications, including food, medical, and household applications. In the current study, we have investigated the fragmentation behavior of EDTA and various metal complexes under collision‐induced dissociation (CID), infrared‐multiphoton dissociation (IRMPD), and higher‐energy collisional dissociation (HCD) activation conditions. Both, positive and negative mode electrospray ionization (ESI) were applied. The metals used to complex with EDTA ranged from alkaline earth metals, such as sodium and cesium, via calcium, nickel, zinc, aluminum, copper, iron, and indium to yttrium and several lanthanides. Furthermore, the protonated and deprotonated species of EDTA, as well as disodium and trisodium species, have been subjected to fragmentation. The results show that characteristic fragmentations were obtained for EDTA and the metal complexes under the investigated conditions. The use of an ion cyclotron resonance (ICR) and an Orbitrap mass spectrometer, as high resolution‐accurate mass instruments, allowed the assignment of elemental compositions undoubtedly for the vast majority of fragments. Certain trends were observed that trend correlated with the size of the metal and the location within the periodic table.

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Cooking with EDTA reduces nutritional value of Vicia faba beans

Abstract: HighlightsChefs used EDTA to accelerate the cooking time of Vicia faba beans.By ESI-MS analysis, disodium EDTA disappeared after cooking process of beans.Up on EDTA addition during cooking, essential nutrients changed.

Cited by 5 publications

References 21 publications

Toxicity of bean cooking media containing EDTA in mice

Toxicity of bean cooking media containing EDTA in mice

Enhancing Antitumor Efficacy of Cisplatin Low Dose by EDTA in Ehrlich Ascetic Carcinoma Bearing Mice

Fragmentation behavior of EDTA complexes under different activation conditions

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