Converted neural cells: induced to a cure?

Zhang, Weiqi; Duan, Shunlei; Liu, Ying; Xu, Xiuling; Qu, Jing; Zhang, Weizhou; Liu, Guanghui

doi:10.1007/s13238-012-2029-2

Cited by 5 publications

(5 citation statements)

References 70 publications

(78 reference statements)

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“…Moreover, as direct lineage conversion bypasses the pluripotent state, it could theoretically reduce the risk of tumorigenicity after transplantation (Ben-David and Benvenisty, 2011 ). Using distinct sets of transcription factors, direct lineage conversion technology has been applied to produce various cell types, including neurons, hepatocytes and neural stem cells (Vierbuchen et al, 2010 ; Huang et al, 2011 ; Kim et al, 2011 ; Pang et al, 2011 ; Sekiya and Suzuki, 2011 ; Giorgetti et al, 2012 ; Liu et al, 2012 ; Zhang et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Direct conversion of human fibroblasts into retinal pigment epithelium-like cells by defined factors

Zhang

Liu

et al. 2014

Protein Cell

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The generation of functional retinal pigment epithelium (RPE) is of great therapeutic interest to the field of regenerative medicine and may provide possible cures for retinal degenerative diseases, including age-related macular degeneration (AMD). Although RPE cells can be produced from either embryonic stem cells or induced pluripotent stem cells, direct cell reprogramming driven by lineage-determining transcription factors provides an immediate route to their generation. By monitoring a human RPE specific Best1::GFP reporter, we report the conversion of human fibroblasts into RPE lineage using defined sets of transcription factors. We found that Best1::GFP positive cells formed colonies and exhibited morphological and molecular features of early stage RPE cells. Moreover, they were able to obtain pigmentation upon activation of Retinoic acid (RA) and Sonic Hedgehog (SHH) signaling pathways. Our study not only established an ideal platform to investigate the transcriptional network regulating the RPE cell fate determination, but also provided an alternative strategy to generate functional RPE cells that complement the use of pluripotent stem cells for disease modeling, drug screening, and cell therapy of retinal degeneration.Electronic supplementary materialThe online version of this article (doi:10.1007/s13238-013-0011-2) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Direct conversion of human fibroblasts into retinal pigment epithelium-like cells by defined factors

Zhang

Liu

et al. 2014

Protein Cell

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“…Very recent publications describe the direct conversion of mouse somatic cells into induced neural stem cells (iNSCs) [1][2][3][4][5][6]. Together with other reports [7,8], they constitute the basis for a rising concept that priming and retaining reprogrammed cells into an expandable neural progenitor stage may render them to be more amenable and with great potential for both disease modeling and possible therapeutic treatment for neurological diseases.…”

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confidence: 99%

Induced neural stem cells: a new tool for studying neural development and neurological disorders

Liu

Suzuki

et al. 2012

Cell Res

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“…As a result, many groups have sought to establish protocols to efficiently differentiate hESCs/hiPSCs into DA neurons 6,7 . It has been reported that transplantation of these hESCs/hiPSCs derived DA neurons into a rodent model of PD can substantially reverse animal behavioral dysfunction, suggesting a clinical potential of this treatment 8,9,10,11 . Moreover, Wernig and colleagues found that 6-hydroxydopamine (6-OHDA) rodent model of PD showed behavioral improvement after the engraftment of hiPSCs derived DA neurons, which strongly advocates the concept of hiPSCs mediated autologous cell therapy.…”

Section: Stem Cell Based Therapies For Neurological Disordersmentioning

confidence: 99%

Progress and prospects in stem cell therapy

Pan

et al. 2013

Acta Pharmacol Sin

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In the past few years, progress being made in stem cell studies has incontestably led to the hope of developing cell replacement based therapy for diseases deficient in effective treatment by conventional ways. The induced pluripotent stem cells (iPSCs) are of great interest of cell therapy research because of their unrestricted self-renewal and differentiation potentials. Proof of principle studies have successfully demonstrated that iPSCs technology would substantially benefit clinical studies in various areas, including neurological disorders, hematologic diseases, cardiac diseases, liver diseases and etc. On top of this, latest advances of gene editing technologies have vigorously endorsed the possibility of obtaining disease-free autologous cells from patient specific iPSCs. Here in this review, we summarize current progress of stem cell therapy research with special enthusiasm in iPSCs studies. In addition, we compare current gene editing technologies and discuss their potential implications in clinic application in the future.

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Converted neural cells: induced to a cure?

Cited by 5 publications

References 70 publications

Direct conversion of human fibroblasts into retinal pigment epithelium-like cells by defined factors

Direct conversion of human fibroblasts into retinal pigment epithelium-like cells by defined factors

Induced neural stem cells: a new tool for studying neural development and neurological disorders

Progress and prospects in stem cell therapy

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