Conversion rates of five polycyclic aromatic hydrocarbons in liquid cultures of fifty-eight fungi and the concomitant production of oxidative enzymes

Gramss, G.; Kirsche, Brigitta; Voigt, Kerstin; Günther, Th.; Fritsche, W.

doi:10.1017/s0953756298008144

Cited by 82 publications

(39 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Following a 3 weeks' incubation at 16 -20 °C in a greenhouse, 10 ml of toluene extractant were added. Toluene aliquots of 1 ml were taken after 48 h and analyzed by HPLC (Kontron System, model 322 with fluorescence detection), compare [31].…”

Section: Degradation Of Pahs In Fungus-plant Microcosmsmentioning

confidence: 99%

“…In regard of basidiomycetes, attention was paid to increases in the production of oxidoreductases [29] and in the mineralization of 2,4-dichlorophenol [30] by ectomycorrhizal fungi in symbiosis with pine. Molecular effects of common liquid and volatile plant emanations on enzymatic performance and catabolism of (xenobiotic) aromatics by basidiomycetous soil saprobes with their white-rot-type oxidoreductase costume [e.g., 31] have not been shown.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Mutual influence of soil basidiomycetes and white mustard plants on their enzymatic and catabolic activities

Gramss

Mascher

2010

J. Basic Microbiol.

View full text Add to dashboard Cite

Liquid and volatile emanations in interactions of soil basidiomycetes with herbs affect fungal oxidoreductases and stress-related plant peroxidases (PO). In this study, gnotobiotic co-cultures between 6 non-pathogenic saprobes and 2 ectomycorrhizal basidiomycetes with the non-host plant white mustard were established on glucose-salt medium with the respective controls. Determined were oxidoreductase activities for culture fluids and plant tissues at initial fungal idiophase and degradation rates of Remazol-BBR and 5 PAHs. In culture fluids of Agaricus arvensis, A. porphyrizon, Lepista nebularis, Stropharia rugoso-annulata, and Hypholoma fasciculare (group-5), the laccase-deficient plant enabled activity increases in fungal laccase (by 2300-fold), in extracellular (fungal and?) plant-derived peroxidases (by 21-fold), and in the dissipation of phenanthrene and anthracene. Oxidative activities in roots rose by 46000-fold during adsorption of fungal laccases. Increases in the stress-related shoot-PO (by 4.1-fold) were exclusively elicited by group-5 saprobes and correlated with plant-phenolic-mediated formations of Mn(III) and increases in Remazol BBR bleaching. Agaricus bisporus and the ectomycorrhizal Hebeloma crustuliniforme and Suillus granulatus did not respond to plant emanations with elevated laccase activities but solubilized apparently root-surface PO. They failed to elicit stress-related activity increases of PO in white mustard shoot and prevented Mn(III) formation in several tissues. It is concluded that white mustard emanations promoted the catabolic performance of the plant-stress eliciting group-5 saprobes but not of A. bisporus and the ectomycorrhizal fungi with their low stress-inducing potential. The nature of the plant-released stimuli and the classes of fungus-released stress agents discussed must be determined in further studies.

show abstract

Section: Degradation Of Pahs In Fungus-plant Microcosmsmentioning

confidence: 99%

mentioning

confidence: 99%

Mutual influence of soil basidiomycetes and white mustard plants on their enzymatic and catabolic activities

Gramss

Mascher

2010

J. Basic Microbiol.

View full text Add to dashboard Cite

show abstract

“…There is, however, a second ecophysiological group of ligninolytic basidiomycetes-the litter-decomposing fungi-which have recently been shown to possess a ligninolytic enzyme system similar to that of white-rot fungi (48,49). Furthermore, screening tests have demonstrated that litter-decomposing fungi are capable of metabolizing PAHs, including BaP, to some extent (21,22,47,56).In the present study, we report the degradation of BaP by the litter-decomposing fungus Stropharia coronilla, which preferably inhabits grasslands. The species is a typical grass dweller that colonizes pastures, meadows, and waysides and is found in both Europe and North America (8,40).…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Degradation of Benzo[ a ]pyrene by the Litter-Decomposing Basidiomycete Stropharia coronilla : Role of Manganese Peroxidase

Steffen

Hatakka

Hofrichter

2003

Appl Environ Microbiol

View full text Add to dashboard Cite

The litter-decomposing basidiomycete Stropharia coronilla, which preferably colonizes grasslands, was found to be capable of metabolizing and mineralizing benzo Benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon (PAH) consisting of five fused benzene rings, is known to be carcinogenic and mutagenic (10,12). Together with other PAHs, BaP is commonly formed during the pyrolysis and incomplete combustion of biological material and organic compounds and is found in various concentrations in coal tar, petroleum, and oil-based fuels (42). Thus, soils from gasworks sites and carbochemical plants as well as refineries and filling stations are often contaminated with BaP. This causes an obvious health risk that has raised the public interest in the fate and the removal of BaP and similar compounds in and from the environment.PAHs with more than four rings are considered highly recalcitrant and resistant to microbial degradation (10). In addition, the higher their molecular weight is, the lower is their water solubility and thus their bioavailability (54). While lowermolecular-mass PAHs-e.g., naphthalene, anthracene, and phenanthrene-are readily degraded by a number of aerobic bacteria, which can utilize these compounds as carbon sources (10,12,33), BaP and other PAHs with high molecular masses are cometabolically degraded by only a few bacterial species (Mycobacterium spp. and Sphingomonas spp.) and mixed microbial cultures (33).In addition to certain bacteria, wood-colonizing basidiomycetes such as Phanerochaete chrysosporium and Bjerkandera sp. strain BOS55 have been shown to metabolize BaP in liquid culture and soil (9, 24, 36). These white-rot fungi produce extracellular ligninolytic oxidoreductases, namely, manganese peroxidase (MnP), lignin peroxidase (LiP), and laccase, which attack aromatic substances, including PAHs, via the formation of free radicals (25,26,35). Since these fungi specialize in colonizing compact wood (timber and stumps) and cannot compete in soil for a prolonged time, their actual contribution to the removal of recalcitrant PAHs under natural conditions seems to be limited. There is, however, a second ecophysiological group of ligninolytic basidiomycetes-the litter-decomposing fungi-which have recently been shown to possess a ligninolytic enzyme system similar to that of white-rot fungi (48,49). Furthermore, screening tests have demonstrated that litter-decomposing fungi are capable of metabolizing PAHs, including BaP, to some extent (21,22,47,56).In the present study, we report the degradation of BaP by the litter-decomposing fungus Stropharia coronilla, which preferably inhabits grasslands. The species is a typical grass dweller that colonizes pastures, meadows, and waysides and is found in both Europe and North America (8,40). Particular attention is paid to the role of MnP, the predominant ligninolytic enzyme of this fungus, and the results indicate its crucial role in the degradation of PAH. MATERIALS AND METHODSOrganism, culture conditions, and enzyme preparation. The litter-decompos...

show abstract

“…could be successfully introduced into untreated soils or those amended with lignocelluloses or minerals (e.g., Scheibner 1998). Due to a widespread lack in lignin peroxidase and low manganese peroxidase activities (Gramss et al 1999, Leonowicz et al 2001, the potential of basidiomycetous ground fungi to degrade xenobiotic hydrocarbons has poorly been studied (Gramss et al 1999, Scheibner 1998, although their life span in soil should suffice to do so. The life span of a single fungal colony will not suffice to control soil heavy metal contaminations in cooperation with phytoextractive crops for more than one rotation in spite of their significant impact on sequestration, solubilization, complexation, and plant uptake of metal cations.…”

Section: Technological Aspectsmentioning

confidence: 99%

Microbial competition, lack in macronutrients, and acidity as main obstacles to the transfer of basidiomycetous ground fungi into (organically or heavy‐metal contaminated) soils

Gramss

Bergmann

2007

J. Basic Microbiol.

View full text Add to dashboard Cite

Non-symbiotic soil microorganisms which have been expensively engineered or selected to support plant nutrition, control root diseases, degrade xenobiotic hydrocarbons, and repress or stimulate heavy metal uptake of plants fail to survive in target soils. This prompted studies into the role of chemistry and microbial pre-colonization of 23 top soils in long-term growth of basidiomycetes. Fungi are seen as auxiliary agents in soil remediation. Untreated soils (1.5 L) were colonized by lignocellulose preferring ground fungi such as Agaricus aestivalis, A. bisporus, A. campestris, A. edulis, A. macrocarpus, A. porphyrizon, Agrocybe dura, A. praecox, Clitocybe sp., Coprinus comatus, Lepista nuda, L. sordida, Macrolepiota excoriata, M. procera, Stropharia coronilla, and S. rugoso-annulata. Spawn mycelia of fairy-ring-type fungi such as Agaricus arvensis, A. fissuratus, A. langei, A. lanipes, A. pilatianus, Lyophyllum sp., and Marasmius oreades died back in contact with non-sterile soils. Fungal growth correlated positively with the soils' Ct Ca K Mg content and negatively with microbial CO2 evolution. Pasteurization and autoclaving increased mycelial growth and life span in soils pH 6.6-8.2. Growth of pH-sensitive but not of pH-tolerant fungi was inhibited on the Ca-deficient soils pH 4-4.4 (-5.6) and was not improved by autoclaving. The pretended fungistasis of acid soils to pH-sensitive fungi was controlled by N P K mineral (pH not altering) or organic (pH increasing) fertilizing as well as by neutralization with NaOH or CaCO3. Although microbial competition was mortal to 33% of the fungal mycelia inserted into natural unplanted soils, further seriously antifungal effects beyond those pretended by low pH conditions and shortage in mineral macronutrients were not identified.

show abstract

Conversion rates of five polycyclic aromatic hydrocarbons in liquid cultures of fifty-eight fungi and the concomitant production of oxidative enzymes

Cited by 82 publications

References 48 publications

Mutual influence of soil basidiomycetes and white mustard plants on their enzymatic and catabolic activities

Mutual influence of soil basidiomycetes and white mustard plants on their enzymatic and catabolic activities

Degradation of Benzo[ a ]pyrene by the Litter-Decomposing Basidiomycete Stropharia coronilla : Role of Manganese Peroxidase

Microbial competition, lack in macronutrients, and acidity as main obstacles to the transfer of basidiomycetous ground fungi into (organically or heavy‐metal contaminated) soils

Contact Info

Product

Resources

About