Conversion of woody biomass into fermentable sugars by cellulase from Agaricus arvensis

Jeya, Marimuthu; Nguyen, Ngoc-Phuong-Thao; Moon, Hee-Jung; Kim, Sang‐Hwan; Lee, Jung-Kul

doi:10.1016/j.biortech.2010.06.055

Cited by 46 publications

(20 citation statements)

References 32 publications

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“…Optimal saccharification conditions were obtained using 1% of the acid pretreated SSS, 25 FPU/g dry substrate in house cellulase, pH 5 and a temperature of 50 °C, which provided a maximum yield of 0.440 g glucose/g dry substrate (Run No.26 in Table 4) at 72 h incubation time. Optimal saccharification conditions of several commercial cellulase and substrate concentrations were in the range 15-35 FPU/g dry substrate and 1.0-5.0% w/v, respectively (Cara et al, 2008;Jeya et al, 2010).…”

Section: Optimization Of Cellulase Saccharification Conditions From Cmentioning

confidence: 99%

“…A statistical method was recently used as an alternative and more efficient approach. Response surface methodology (RSM) was applied to identify optimal conditions for reducing sugar production from enzyme saccharification of pretreated sweet sorghum straw by analyzing the effect of multiple variables on overall process speed and efficiently with minimal experiments while ensuring a high degree of statistical significance in the results (Qi et al, 2009;Levin et al, 2008;Jeya et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Optimization of Saccharification Conditions of Acid-pretreated Sweet Sorghum Straw Using Response Surface Methodology

Phuengjayaem¹,

Poonsrisawat²,

Petsom³

et al. 2014

JAS

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This study focused on the cellulase production from C. versicolor TD17, white rot fungi. The maximum cellulase activity of 0.897 U/ml was obtained after 5 days of cultivation using 20 g/l cellobiose as a carbon source and 2 g/l ammonium sulfate supplemented with 0.3 g/l urea as nitrogen sources. Enzymatic saccharification of acid-pretreated sweet sorghum straw (SSS) using in house cellulase was optimized using Response Surface Methodology (RSM), variable five-code-level, four-factor; 1.0-7.0% w/v acid-pretreated SSS, 15-35 FPU/g dry substrate of cellulase enzyme, pH 3 to 7 and temperatures 30 to 70 °C. The optimal conditions were 1% w/v acid-pretreated SSS, 25 FPU/g dry substrate of cellulase, pH 5, 50 °C and 72 h cultivation. A maximal glucose yield of 0.440 g/g dry substrate was obtained.

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Section: Optimization Of Cellulase Saccharification Conditions From Cmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optimization of Saccharification Conditions of Acid-pretreated Sweet Sorghum Straw Using Response Surface Methodology

Phuengjayaem¹,

Poonsrisawat²,

Petsom³

et al. 2014

JAS

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“…With this strategy, the effect of an individual parameter on enzymes production were evaluated, and the optimum conditions were identified and used in the subsequent studies. For chemical parameter screening, 1% (w/v) concentration was used for all chemical elements based on results of previous studies …”

Section: Methodsmentioning

confidence: 99%

Comprehensive studies on optimization of cellulase and xylanase production by a local indigenous fungus strain via solid state fermentation using oil palm frond as substrate

Tai

Tan

Lim

et al. 2019

Biotechnology Progress

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The high cost of cellulases remains the most significant barrier to the economical production of bio-ethanol from lignocellulosic biomass. The goal of this study was to optimize cellulases and xylanase production by a local indigenous fungus strain (Aspergillus niger DWA8) using agricultural waste (oil palm frond [OPF]) as substrate.The enzyme production profile before optimization indicated that the highest carboxymethyl cellulose (CMCase), filter paper (FPase), and xylanase activities of 1.06 U/g, 2.55 U/g, and 2.93 U/g were obtained on day 5, day 4, and day 5 of fermentation, respectively. Response surface methodology was used to study the effects of several key process parameters in order to optimize cellulase production. Of the five physical and two chemical factors tested, only moisture content of 75% (w/w) and substrate amount of 2.5 g had statistically significant effect on enzymes production. Under optimized conditions of 2.5 g of substrate, 75% (w/w) moisture content, initial medium of pH 4.5, 1 × 10 6 spores/mL of inoculum, and incubation at ambient temperature (±30 C) without additional carbon and nitrogen, the highest CMCase, FPase, and xylanase activities obtained were 2.38 U/g, 2.47 U/g, and 5.23 U/g, respectively. Thus, the optimization process increased CMCase and xylanase production by 124.5 and 78.5%, respectively. Moreover, A. niger DWA8 produced reasonably good cellulase and xylanase titers using OPF as the substrate when compared with previous researcher finding. The enzymes produced by this process could be further use to hydrolyze biomass to generate reducing sugars, which are the feedstock for bioethanol production. K E Y W O R D SAspergillus niger, cellulases, oil palm frond, response surface methodology, xylanase

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“…El valor máximo de azúcares reductores obtenidos (1.81 g/L) fue comparable al obtenido por Jeya et al (2010) con biomasa leñosa y una carga enzimática de 20 unidades de papel de filtro, (FPU, por sus siglas en inglés)/g sustrato (2.9 g azúcares/L a las 50 h de sacarificación). Yamashita et al (2010) no observaron producción de glucosa y azúcares reductores en la sacarificación enzimática del bambú sin pretratar.…”

Section: Sacarificaciónunclassified

“…Respecto al tiempo de sacarificación, la mayor concentración de azúcares se obtuvo entre las 24 -48 h (1.21 g/L en promedio), datos que coinciden con lo reportado por otros autores (Dogaris et al 2009, López-Miranda et al 2009, Jeya et al 2010.…”

Section: Sacarificaciónunclassified

Obtención De Azúcares Fermentables a Partir De Aserrín De Pino Pretratado Secuencialmente Con Ácido-Base

Rodríguez

Castrillo

Velázquez

et al. 2017

Rev. Int. Contam. Ambie.

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Palabras clave: residuo lignocelulósico, diseño factorial, lignina, sacarificación. RESUMENEl uso de residuos lignocelulósicos constituye una fuente abundante y de bajo costo, que genera, además, una solución al problema de su disposición final. El objetivo del trabajo fue evaluar el efecto de distintas condiciones de pretratamiento secuencial ácido-base de aserrín de pino, sobre la liberación de azúcares reductores en la etapa de sacarificación. El material lignocelulósico se pretrató en dos etapas: la primera, se realizó con ácido sulfúrico (PT1) y la segunda con hidróxido de sodio (PT2), ambas con tres niveles: 1, 2 y 3 % mediante un diseño 3 2 factorial. La hidrólisis enzimática se realizó a 50 ºC y 150 rpm en frascos de 50 mL. Se tomaron muestras a las 0.15, 24, 48, 72 y 96 h. Se determinó el porcentaje de lignina ácido soluble y la concentración de azúcares reductores. El nivel óptimo de cada factor que favoreció la mayor deslignificación correspondió a 3 % de ácido sulfúrico y 3 % de hidróxido de sodio. La producción de azúcares reductores no se vio afectada por el PT2, pero sí por el PT1, alcanzando un máximo para el nivel 0 del mismo (2 % de ácido sulfúrico). Se optimizó el pretratamiento secuencial ácido-base y se comprobó además que éste mejora la hidrólisis enzimática respecto al aserrín de pino sin pretratar. La cantidad de azúcares reductores obtenidos fue comparable a la obtenida por otros autores.Key words: lignocellulosic waste, factorial design, lignin, saccharification. ABSTRACTThe use of lignocellulosic waste constitutes an abundant and inexpensive source; it also offers a solution to the problem of waste disposal. The aim of the study was to evaluate the effect of different conditions of a sequential acid-base pretreatment of pine sawdust, through the release of reducing sugars in the saccharification step. The lignocellulosic material was pretreated in two stages: acid pretreatment (PT1), using sulfuric acid and basic pretreatment (PT2), employing sodium hydroxide at three levels each one: 1, 2 and 3 % in a factorial design. Enzymatic hydrolysis was carried out at 50 ºC and 150 rpm in 50 mL vials. Samples were taken at 0.15, 24, 48, 72 and 96 h. The percentage of acid soluble lignin and the concentration of reducing sugars were determined. The Rev. Int.

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Conversion of woody biomass into fermentable sugars by cellulase from Agaricus arvensis

Cited by 46 publications

References 32 publications

Optimization of Saccharification Conditions of Acid-pretreated Sweet Sorghum Straw Using Response Surface Methodology

Optimization of Saccharification Conditions of Acid-pretreated Sweet Sorghum Straw Using Response Surface Methodology

Comprehensive studies on optimization of cellulase and xylanase production by a local indigenous fungus strain via solid state fermentation using oil palm frond as substrate

Obtención De Azúcares Fermentables a Partir De Aserrín De Pino Pretratado Secuencialmente Con Ácido-Base

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