Conversion of Waste Cooking Oil to Rhamnolipid by a Newly Oleophylic Pseudomonas aeruginosa WO2

Shi, Shu Zhi; Teng, Zedong; Liu, Jianwei; Li, Tinggang

doi:10.3390/ijerph19031700

Cited by 10 publications

(5 citation statements)

References 44 publications

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“…Media originated from agro-industrial and/or domestic waste with high carbon availability and/or providing increased biosurfactant production and stability, such as bagasse and potato peels (24), rice bran oil residue (25) and residual frying oil (52) have been extensively investigated. These options comprise alternatives to nancially compete with traditional surfactants (47), which have established economies of scale and are produced in large quantities.…”

Section: Resultsmentioning

confidence: 99%

Biosurfactant production by Pseudomonas: a systematic review

Araujo,

Santana,

Otenio

et al. 2024

Preprint

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It is of fundamental interest to research and develop innovative biotechnologies, as well as bioproducts that replace or are alternatives to those of non-renewable origin, such as biosurfactants in relation to traditional surfactants used in various sectors. Consequently, there are a large number of experimental studies addressing different subjects, especially with the use of bacteria of the genus Pseudomonas, however, there is a lack of work that demonstrates the evaluation of this science produced to date. Therefore, this article discusses the production of biosurfactants by Pseudomonas with the aim of surveying and analyzing experimental articles on this topic. To realize this, a systematic search was carried out with well-defined temporal space, databases and inclusion and exclusion criteria, based on metric studies that guided what information would be collected and the method of evaluation. Therefore, a large number of articles were selected, which demonstrated Pseudomonas aeruginosa as the bioagent mostly used in the tests, which aimed to improve the process in the area. Furthermore, interest in this field has increased over the years, predominantly in emerging market countries, where the most prominent authors on the topic are found. Therefore, it is necessary that there is an expansion of interest in the area to make the production of biosurfactants cheaper in areas that currently have greater development deficiencies, such as means of purifying the bioprocess and reducing foam formation in the bioprocess.

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Section: Resultsmentioning

confidence: 99%

Biosurfactant production by Pseudomonas: a systematic review

Araujo,

Santana,

Otenio

et al. 2024

Preprint

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“…Critical micelle concentration (CMC) of the produced biosurfactant value was almost half that reported by Shawkat et al [ 26 ] and Kumari et al [ 27 ]. A low CMC indicates that this compound can exert its effect at low concentrations [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

“…The hydrophilic component can be a carbohydrate or a protein. The diverse structural variations imply a variety of physicochemical properties [ 8 ]. Biosurfactants are perceived as the next generation multifunctional biomolecules that can be used for an array of applications such as medicine, industry, cosmetics and bioremediation.…”

Section: Introductionmentioning

confidence: 99%

Valorization of frying oil waste for biodetergent production using Serratia marcescens N2 and gamma irradiation assisted biorecovery

Elkenawy

Gomaa

2022

Microb Cell Fact

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Background The complexity, toxicity and abundance of frying oil waste (FOW) render it difficult to be degraded biologically. The aim of the present work was to valorize FOW and investigate the potential use of the produced biosurfactant by Serratia marcescens N2 (Whole Genome sequencing accession ID SPSG00000000) as a biodetergent. Results Serratia marcescens N2 demonstrated efficient valorization of FOW, using 1% peptone, 20% FOW and 8% inoculum size. Gene annotation showed the presence of serrawettin synthetase indicating that the produced biosurfactant was serrawettin. Zeta potential and Fourier Transform Infrared (FTIR) spectroscopy indicate that the biosurfactant produced was a negatively charged lipopeptide. The biosurfactant reduced the surface tension of water from 72 to 25.7 mN/m; its emulsification index was 90%. The valorization started after 1 h of incubation and reached a maximum of 83.3%. Gamma radiation was used to increase the biosurfactant yield from 9.4 to 19.2 g/L for non-irradiated and 1000 Gy irradiated cultures, respectively. It was noted that the biorecovery took place immediately as opposed to overnight storage required in conventional biosurfactant recovery. Both chemical and functional characteristics of the radiation induced biosurfactant did not change at low doses. The produced biosurfactant was used to wash oil stain; the highest detergency reached was 87% at 60 °C under stirring conditions for 500 Gy gamma assisted biorecovery. Skin irritation tests performed on experimental mice showed no inflammation. Conclusion This study was able to obtain a skin friendly effective biodetergent from low worth FOW using Serratia marcescens N2 with 83% efficient valorization using only peptone in the growth media unlike previous studies using complex media. Gamma radiation was for the first time experimented to assist biosurfactant recovery and doubling the yield without affecting the efficiency.

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“…The production of rhamnolipids was first described nearly 70 years ago for the human pathogen Pseudomonas aeruginosa [ 20 , 21 , 22 , 23 , 24 , 25 , 26 ], which represents the best-known native producer strain until today. Apart from P. aeruginosa , several bacteria from the genus Burkholderia are known producers of rhamnolipids too.…”

Section: Introductionmentioning

confidence: 99%

Recombinant Production of Pseudomonas aeruginosa Rhamnolipids in P. putida KT2440 on Acetobacterium woodii Cultures Grown Chemo-Autotrophically with Carbon Dioxide and Hydrogen

Widberger,

Wittgens,

Klaunig

et al. 2024

Microorganisms

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The establishment of sustainable processes for the production of commodity chemicals is one of today’s central challenges for biotechnological industries. The chemo-autotrophic fixation of CO2 and the subsequent production of acetate by acetogenic bacteria via anaerobic gas fermentation represents a promising platform for the ecologically sustainable production of high-value biocommodities via sequential fermentation processes. In this study, the applicability of acetate-containing cell-free spent medium of the gas-fermenting acetogenic bacterium A. woodii WP1 as the feeder strain for growth and the recombinant production of P. aeruginosa PAO1 mono-rhamnolipids in the well-established nonpathogenic producer strain P. putida KT2440 were investigated. Additionally, the potential possibility of a simplified production process without the necessary separation of feeder strain cells was elucidated via the cultivation of P. putida in cell-containing A. woodii culture broth. For these cultures, the content of both strains was investigated by examining the relative quantification of strain-exclusive genes via qPCR. The recombinant production of mono-rhamnolipids was successfully achieved with maximum titers of approximately 360–400 mg/L for both cell-free and cell-containing A. woodii spent medium. The reported processes therefore represent a successful proof of principle for gas fermentation-derived acetate as a potential sustainable carbon source for future recombinant rhamnolipid production processes by P. putida KT2440.

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Conversion of Waste Cooking Oil to Rhamnolipid by a Newly Oleophylic Pseudomonas aeruginosa WO2

Cited by 10 publications

References 44 publications

Biosurfactant production by Pseudomonas: a systematic review

Biosurfactant production by Pseudomonas: a systematic review

Valorization of frying oil waste for biodetergent production using Serratia marcescens N2 and gamma irradiation assisted biorecovery

Recombinant Production of Pseudomonas aeruginosa Rhamnolipids in P. putida KT2440 on Acetobacterium woodii Cultures Grown Chemo-Autotrophically with Carbon Dioxide and Hydrogen

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