Conversion of soluble dopamine β-hydroxylase to a membrane binding form

Taylor, C S; Fleming, Patrick

doi:10.1016/s0021-9258(19)84816-0

Cited by 13 publications

(2 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DBH is a rate-limiting enzyme in catecholamine synthesis and plays a key role in converting DA to norepinephrine (Kaufman, 1974;Perez-Polo, Tiffany-Castiglioni, Ziegler, & Werrbach-Perez, 1982) Winkler, 1976). Taylor and Fleming (1989) showed that sDBH can convert into mDBH with the appropriate pH and vesicle phosphatidylserine composition. We found that DBH expression increased in lipid rafts following GDNF treatment.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Glial cell line‐derived neurotrophic factor alters lipid composition and protein distribution in MPP+‐injured differentiated SH‐SY5Y cells

Liu

Jiang

et al. 2020

Journal Cellular Physiology

View full text Add to dashboard Cite

Parkinson's disease (PD) is a neurodegenerative disease characterized by progressive loss of dopaminergic neurons in the substantia nigra and striatum. Glial cell line‐derived neurotrophic factor (GDNF) can effectively promote the differentiation and survival of many types of neurons, especially dopaminergic neurons, suggesting it could be a treatment for PD. Lipid rafts are highly dynamic cell membrane domains that contain numerous signal protein receptors, providing an important platform for signal transduction. Compelling evidence indicates that alterations in lipid rafts are associated with PD, and some studies have reported that GDNF can regulate the expression of caveolin‐1, a lipid raft‐marker protein. However, the precise effects of GDNF on lipid rafts remain unknown. We developed a cellular PD model, purified detergent‐resistant membranes (membrane rafts), and performed proteomic and lipid metabolomics analyses to examine changes in lipid rafts after GDNF treatment. The results showed considerable protein and lipid alterations in response to GDNF, especially altered levels of dopamine‐β‐hydroxylase, heat shock 70 kDa protein, neural cell adhesion molecule, cytoskeletal proteins, and long‐chain polysaturated/unsaturated fatty acids. These findings reveal a new avenue to explore the relationships between GDNF, lipid rafts, and PD and support the hypothesis that GDNF may be a useful treatment for PD.

show abstract

Section: Discussionmentioning

confidence: 99%

“…It exists in two forms: soluble DBH (sDBH) and membrane‐bound DBH (mDBH; Smith & Kirshner, 1967; Winkler, 1976). Taylor and Fleming (1989) showed that sDBH can convert into mDBH with the appropriate pH and vesicle phosphatidylserine composition. We found that DBH expression increased in lipid rafts following GDNF treatment.…”

Section: Discussionmentioning

confidence: 99%