Conversion of <scp>D</scp>‐xylose to ethanol by the yeast <i>Pachysolen tannophilus</i>

Slininger, Patricia J.; Bothast, Rodney J.; Cauwenberge, J. E. Van; Kurtzman, Cletus P.

doi:10.1002/bit.260240210

Cited by 234 publications

(68 citation statements)

References 10 publications

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“…The earliest observations on xylose fermentation by yeasts stem from the early 1980's. Yeasts like Pachysolen tannophilus (Slininger et al 1982) and Candida tropicalis (Gong et al 1981a) are able to ferment xylose to ethanol. In an extensive screening of 200 yeast strains able to grow on xylose, nineteen were found that produced 0.1-1.0 g l -1 of ethanol under fermentative conditions from 20 g l -1 xylose.…”

Section: Xylose Fermentationmentioning

confidence: 99%

Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: current status

Maris

Abbott

Bellissimi

et al. 2006

Antonie van Leeuwenhoek

429

276

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Fuel ethanol production from plant biomass hydrolysates by Saccharomyces cerevisiae is of great economic and environmental significance. This paper reviews the current status with respect to alcoholic fermentation of the main plant biomass-derived monosaccharides by this yeast. Wild-type S. cerevisiae strains readily ferment glucose, mannose and fructose via the Embden-Meyerhof pathway of glycolysis, while galactose is fermented via the Leloir pathway. Construction of yeast strains that efficiently convert other potentially fermentable substrates in plant biomass hydrolysates into ethanol is a major challenge in metabolic engineering. The most abundant of these compounds is xylose. Recent metabolic and evolutionary engineering studies on S. cerevisiae strains that express a fungal xylose isomerase have enabled the rapid and efficient anaerobic fermentation of this pentose.L-Arabinose fermentation, based on the expression of a prokaryotic pathway in S. cerevisiae, has also been established, but needs further optimization before it can be considered for industrial implementation. In addition to these already investigated strategies, possible approaches for metabolic engineering of galacturonic acid and rhamnose fermentation by S. cerevisiae are discussed. An emerging and major challenge is to achieve the rapid transition from proof-of-principle experiments under 'academic' conditions (synthetic media, single substrates or simple substrate mixtures, absence of toxic inhibitors) towards efficient conversion of complex industrial substrate mixtures that contain synergistically acting inhibitors.

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Section: Xylose Fermentationmentioning

confidence: 99%

Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: current status

Maris

Abbott

Bellissimi

et al. 2006

Antonie van Leeuwenhoek

429

276

View full text Add to dashboard Cite

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“…The interest in studying catabolic pathway of xylose utilization has been grown [28][29][30], with the discovery of pentose-fermenting yeast strains [31,32]. The bio-renewable chemicals and fuels fermentative production need the biocatalysts engineering that can quickly and efficiently transform sugars to the target products with lower cost than the existing petrochemical-based processes.…”

Section: Introductionmentioning

confidence: 99%

“…Also, the yeast Candida utilis is strictly aerobic and can grow on xylose, but it is not an ethanol producing strain. Early in 1980, after identification the ability of Schizosaccharomyces pombe, S. cerevisiae and other yeast strains to ferment D-Xylose to ethanol [70,71], a lot of screening effort rapidly detected that some strains could convert xylose to ethanol immediately under oxygen-limiting or aerobic conditions [32,72]. Nowadays, Pichia stipitis, C. shehatae, and Pachysolen tannophilus attract a lot of attention as the best known natural xylose-fermenting yeasts [73][74][75].…”

Section: Introductionmentioning

confidence: 99%

Bioethanol a Microbial Biofuel Metabolite; New Insights of Yeasts Metabolic Engineering

et al. 2018

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Scarcity of the non-renewable energy sources, global warming, environmental pollution, and raising the cost of petroleum are the motive for the development of renewable, eco-friendly fuels production with low costs. Bioethanol production is one of the promising materials that can subrogate the petroleum oil, and it is considered recently as a clean liquid fuel or a neutral carbon. Diverse microorganisms such as yeasts and bacteria are able to produce bioethanol on a large scale, which can satisfy our daily needs with cheap and applicable methods. Saccharomyces cerevisiae and Pichia stipitis are two of the pioneer yeasts in ethanol production due to their abilities to produce a high amount of ethanol. The recent focus is directed towards lignocellulosic biomass that contains 30-50% cellulose and 20-40% hemicellulose, and can be transformed into glucose and fundamentally xylose after enzymatic hydrolysis. For this purpose, a number of various approaches have been used to engineer different pathways for improving the bioethanol production with simultaneous fermentation of pentose and hexoses sugars in the yeasts. These approaches include metabolic and flux analysis, modeling and expression analysis, followed by targeted deletions or the overexpression of key genes. In this review, we highlight and discuss the current status of yeasts genetic engineering for enhancing bioethanol production, and the conditions that influence bioethanol production.

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“…From the discovery of the ability of certain yeasts to ferment xylose as a source of carbon and energy (Schneider et al, 1981;Slininger et al, 1982), the interest of scientists in xylitol pro duction by biotechnological means has increased worldwide, since this process has several advan tages over the conventional chemical process (Ojamo et al, 1988). In fact, several researchers have pursuing the developm ent of an economical and feasible technique for xylitol bioproduction from lignocellulosic materials.…”

Section: Introductionmentioning

confidence: 99%