Conversion of residual glycerol from biodiesel synthesis into 1,3-propanediol by a new strain of Klebsiella pneumoniae

Rossi, Daniele Misturini; Souza, Elisangela Aquino de; Flôres, Simone Hickmann; Ayub, Marco Antônio Záchia

doi:10.1016/j.renene.2012.12.048

Cited by 27 publications

(8 citation statements)

References 22 publications

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“…However, productivity is negatively affected by both high and low temperatures. These results are in accordance with Rossi et al [20], who observed that the variation of temperature had shown no effect on 1,3-PDO production by K. pneumoniae Blh-1. The results for temperature and pH for 1,3-PDO production found in this work is different from that of Zhang et al [32], who observed that optimal cultivation parameters for temperature and pH using K. pneumoniae XJ-Li were 40 • C and pH 8.0, respectively.…”

Section: Discussionsupporting

confidence: 93%

“…The difference in pH found between the XJ-Li and GLC29 strains could be explained by the origin of the isolates, since XJ-Li was isolated from micro-alkaline soil. A negative effect on 1,3-PDO production when pH was increased was also observed by Rossi et al [20].…”

Section: Discussionsupporting

confidence: 71%

“…Under optimized conditions, the concentration of 1,3-PDO from glycerol was 81.2 g l −1 using molasses as a co-substrate. Rossi et al [20] reported concentrations up to 23.80 g 1,3-PDO l −1 in batch fermentations under controlled pH, while in fed-batch cultivations the 1,3-PDO production was 36.86 g l −1 using a new strain of K. pneumoniae. Sattayasamitsathit et al [29] applied a statistical optimization for the simultaneous production of 1,3-PDO and 2,3-BDO using crude glycerol by a new K. pneumoniae isolate.…”

Section: Introductionmentioning

confidence: 99%

“…One of the main products obtained through microbial bioconversion of glycerol is 1,3-propanediol (1,3-PDO), a polyol with applications in the cosmetics, food, lubricant, and pharmaceutical industries [18][19][20][21]. The development of polypropylene terephthalate (PPT), a thermoplastic with superior physicochemical properties to those of polyethylene terephthalate (PET), used in the production of fabrics, carpets, and engineered plastics has created a new demand for 1,3-propanediol.…”

Section: Introductionmentioning

confidence: 99%

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Production and productivity of 1,3-propanediol from glycerol by Klebsiella pneumoniae GLC29

2015

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a b s t r a c tInterest in the development of the bioproduction of 1,3-propanediol, an important chemical intermediate with various industrial applications, has increased in recent years. Klebsiella pneumoniae is one of the most studied and efficient bacteria for 1,3-propanediol production from glycerol. A new isolate of K. pneumoniae was investigated using response surface methodology by central composite design for the production of 1,3-propanediol using glycerol. The effects of pH, temperature, stirrer speed, and glycerol concentration on the production and productivity of 1,3-propanediol were examined. Considering both production and productivity, the best conditions for glycerol conversion in 1,3-propanediol are: a pH range of 6.9-7.1, a temperature between 33 and 38.5 • C, a stirrer speed of 110-180 rpm, and a glycerol concentration of 39-49 g l −1 . Batch fermentation carried out at a pH of 7.0, a temperature of 35 • C, a stirrer speed of 150 rpm, and a glycerol concentration of 40 g l −1 produced 20.4 g 1,3-propanediol l −1 , with a maximum volumetric productivity of 2.92 g l −1 h −1 and a yield of 0.51 g g −1 . The main byproducts were acetic acid (approximately 7.0 g l −1 ) and formate (approximately 3.7 g l −1 ). The newly isolated K. pneumoniae GLC29 showed potential for the conversion of glycerol into 1,3-propanediol, with high production and productivity.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Production and productivity of 1,3-propanediol from glycerol by Klebsiella pneumoniae GLC29

2015

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“…[18][19][20][21] Environmental concerns about the discharge of agro-industrial residues have put growing pressure on research to develop novel technologies for waste reuse. Cheese whey permeate and raw glycerol, by-products of dairy and biodiesel production, respectively, have the potential to be used in bioprocesses such as in ethanol production, 22 1,3-propanediol, 23 and for SCO. 18,24 Several other lowcost substrates for lipid and ethanol production have been studied, such as whey, 25 rice hulls, 26 agro-industrial wastewater, 27 wheat straw hydrolysate, 28 sugarcane bagasse, 9 and sweet sorghum.…”

Section: Introductionmentioning

confidence: 99%

Oleaginous yeast Meyerozyma guilliermondii shows fermentative metabolism of sugars in the biosynthesis of ethanol and converts raw glycerol and cheese whey permeate into polyunsaturated fatty acids

Fabricio

Valente

Ayub

2019

Biotechnology Progress

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We studied the biotechnological potential of the recently isolated yeast Meyerozyma guilliermondii BI281A to produce polyunsaturated fatty acids and ethanol, comparing products yields using glucose, raw glycerol from biodiesel synthesis, or whey permeate as substrates. The yeast metabolism was evaluated for different C/N ratios (100:1 and 50:1). Results found that M. guilliermondii BI281A was able to assimilate all tested substrates, and the most efficient conversion obtained was observed using raw glycerol as carbon source (C/N ratio 50:1), concerning biomass formation (5.67 g·L−1) and lipid production (1.04 g·L−1), representing 18% of dry cell weight. Bioreactors experiments under pH and aeration‐controlled conditions were conducted. Obtained fatty acids were composed of ~67% of unsaturated fatty acids, distributed as palmitoleic acid (C16:1, 9.4%), oleic acid (C18:1, 47.2%), linoleic acid (C18:2 n−6, 9.6%), and linolenic acid (C18:3 n−3, 1.3%). Showing fermentative metabolism, which is unusual for oleaginous yeasts, M. guilliermondii produced 13.7 g·L−1 of ethanol (yields of 0.27) when growing on glucose medium. These results suggest the promising use of this uncommonly studied yeast to produce unsaturated fatty acids and ethanol using cheap agro‐industrial residues as substrates in bioprocess.

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Biosynthesis of 1,3‐propanodiol and 2,3‐butanodiol from residual glycerol in continuous cell‐immobilized Klebsiella pneumoniae bioreactors

Damasceno

Rossi

Ayub

2022

Biotechnology Progress

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In recent years, residual glycerol from biodiesel synthesis made this chemical a cheap, readily available carbon source to bioprocess, which is also a form to reduce costs in the fuel industry. We propose and describe a bioprocess using fluidized and packedbed continuous bioreactors to convert this residual glycerol into value-added products such as 1,3-propanediol (1,3-PD) and 2,3-butanediol (2,3-BD), largely used in the chemical industry. The bacterium Klebsiella pneumoniae BLh-1, strain isolated by us, was immobilized in the permeable support of polyvinyl alcohol (LentiKats ® ). After testing different dilution rates (D) for all bioreactor configurations, the best obtained productivities of 1,3-PD was 8.69 g L À1 h À1 at a D = 0.45 h À1 , and 2.99 g L À1 h À1 at a D = 0.30 h À1 for 2,3-BD, both in the packed-bed configuration. In the fluidizedbed reactor, the highest productivity values achieved were 4.48 and 1.16 g L À1 h À1 for 1,3-PD and 2,3-BD, respectively, both at D = 0.33 h À1 . These results show the potential of setting up a bioprocess based on continuous cultures using immobilized K. pneumoniae BLh-1 in PVA matrices in order to efficiently convert the abundant surplus of glycerol into commercially important chemicals such as 1,3-PD and 2,3-BD.

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Conversion of residual glycerol from biodiesel synthesis into 1,3-propanediol by a new strain of Klebsiella pneumoniae

Cited by 27 publications

References 22 publications

Production and productivity of 1,3-propanediol from glycerol by Klebsiella pneumoniae GLC29

Production and productivity of 1,3-propanediol from glycerol by Klebsiella pneumoniae GLC29

Oleaginous yeast Meyerozyma guilliermondii shows fermentative metabolism of sugars in the biosynthesis of ethanol and converts raw glycerol and cheese whey permeate into polyunsaturated fatty acids

Biosynthesis of 1,3‐propanodiol and 2,3‐butanodiol from residual glycerol in continuous cell‐immobilized Klebsiella pneumoniae bioreactors

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