Conversion of orange peel to L-galactonic acid in a consolidated process using engineered strains of Aspergillus niger

Kuivanen, Joosu; Dantas, Hugo; Mojžita, Dominik; Mallmann, Edgar; Biz, Alessandra; Krieger, Nádia; Mitchell, David A.; Richard, Peter

doi:10.1186/s13568-014-0033-z

Cited by 28 publications

(15 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present communication, we established a consolidated process for l -rhamnose isolation from naringin and grape fruit peel. The process is similar to the processes of l -galactonate (Kuivanen et al 2014 ) and l -ascorbic acid (Kuivanen et al 2015 ) production from the d -galacturonate fraction in orange peel. The microorganism is producing the enzymes for the hydrolysis of the biomass and is simultaneously removing most of the sugars except for the l -rhamnose in a single process.…”

Section: Discussionsupporting

confidence: 56%

See 1 more Smart Citation

Engineering a filamentous fungus for l-rhamnose extraction

Kuivanen

Richard

2016

AMB Expr

Self Cite

View full text Add to dashboard Cite

l-Rhamnose is a high value rare sugar that is used as such or after chemical conversions. It is enriched in several biomass fractions such as the pectic polysaccharides rhamnogalacturonan I and II and in naringin, hesperidin, rutin, quercitrin and ulvan. We engineered the filamentous fungus Aspergillus niger to not consume l-rhamnose, while it is still able to produce the enzymes for the hydrolysis of l-rhamnose rich biomass. As a result we present a strain that can be used for the extraction of l-rhamnose in a consolidated process. In the process the biomass is hydrolysed to the monomeric sugars which are consumed by the fungus leaving the l-rhamnose.

show abstract

Section: Discussionsupporting

confidence: 56%

“…Thus a mix of different pectic enzymes is needed for pectin hydrolysis. Aspergillus niger is known to be efficient in pectin hydrolysis and is also capable of hydrolyzing pectin from citrus peels in a consolidated bioprocess (Kuivanen et al 2014 ). However, a lower l -rhamnose yield was achieved from RG when compared to naringin.…”

Section: Discussionmentioning

confidence: 99%

Engineering a filamentous fungus for l-rhamnose extraction

Kuivanen

Richard

2016

AMB Expr

Self Cite

View full text Add to dashboard Cite

show abstract

“…As a result, 3.1 gl −1 galactaric acid was produced from 37.4 gl −1 (dry mass) orange processing waste by the ∆gaaA - ∆39114 - udh . The content of d -galUA in the waste is about 27% [15] resulting in a maximum theoretical galactaric acid concentration of around 10 gl −1 that can be achieved. In addition to galactaric acid, 8.4 gl −1 free d -galUA was observed in the cultivations after 120 h. The sum of observed galactaric acid and d -galUA corresponds approximately to the total d -galUA content in the orange processing waste.…”

Section: Resultsmentioning

confidence: 99%

Engineering Aspergillus niger for galactaric acid production: elimination of galactaric acid catabolism by using RNA sequencing and CRISPR/Cas9

2016

Self Cite

View full text Add to dashboard Cite

Background meso-Galactaric acid is a dicarboxylic acid that can be produced by the oxidation of d-galacturonic acid, the main constituent of pectin. Mould strains can be engineered to perform this oxidation by expressing the bacterial enzyme uronate dehydrogenase. In addition, the endogenous pathway for d-galacturonic acid catabolism has to be inactivated. The filamentous fungus Aspergillus niger would be a suitable strain for galactaric acid production since it is efficient in pectin hydrolysis, however, it is catabolizing the resulting galactaric acid via an unknown catabolic pathway.ResultsIn this study, a transcriptomics approach was used to identify genes involved in galactaric acid catabolism. Several genes were deleted using CRISPR/Cas9 together with in vitro synthesized sgRNA. As a result, galactaric acid catabolism was disrupted. An engineered A. niger strain combining the disrupted galactaric and d-galacturonic acid catabolism with an expression of a heterologous uronate dehydrogenase produced galactaric acid from d-galacturonic acid. The resulting strain was also converting pectin-rich biomass to galactaric acid in a consolidated bioprocess.ConclusionsIn the present study, we demonstrated the use of CRISPR/Cas9 mediated gene deletion technology in A. niger in an metabolic engineering application. As a result, a strain for the efficient production of galactaric acid from d-galacturonic acid was generated. The present study highlights the usefulness of CRISPR/Cas9 technology in the metabolic engineering of filamentous fungi.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-016-0613-5) contains supplementary material, which is available to authorized users.

show abstract

“…d ‐Galacturonic acid, the principal monomer of pectin, has been converted into potential platform chemicals, which include meso ‐galactarate, keto‐deoxy‐ l ‐galactonate, and l ‐galactonic acid, by using engineered filamentous fungi. Recently, a consolidated bioprocess was developed to generate l ‐galactonic acid directly from orange peel . In this work, we developed a new route to produce adipic acid from sugar beet residue by combining biological catalysis and chemical catalysis (Scheme ).…”

Section: Methodsmentioning

confidence: 99%

Production of Adipic Acid from Sugar Beet Residue by Combined Biological and Chemical Catalysis

Zhang¹,

Su³

et al. 2016

ChemCatChem

View full text Add to dashboard Cite

Adipic acid is one of the most important industrial dicarboxylic acids and is used mainly as a precursor to nylon‐6,6. Currently, commercial adipic acid is produced primarily from benzene by a chemical route that is associated with environmental, health, and safety concerns. Herein, we report a new process to produce adipic acid from an inexpensive renewable feedstock, sugar beet residue by combining an engineered Escherichia coli strain and Re‐based chemical catalysts. The engineered E. coli converted d‐galacturonic acid to mucic acid, which was precipitated easily with acid, and the mucic acid was further converted to adipic acid by a deoxydehydration reaction catalyzed by an oxorhenium complex followed by a Pt/C‐catalyzed hydrogenation reaction under mild conditions. A high selectivity to the free acid products was achieved by tuning the acidity of the Re‐based catalysts. Finally, adipic acid was produced directly from sugar beet residue that was hydrolyzed enzymatically with engineered E. coli and two chemical catalysts in a yield of 8.4 %, which signifies a new route for the production of adipic acid.

show abstract

Conversion of orange peel to L-galactonic acid in a consolidated process using engineered strains of Aspergillus niger

Cited by 28 publications

References 24 publications

Engineering a filamentous fungus for l-rhamnose extraction

Engineering a filamentous fungus for l-rhamnose extraction

Engineering Aspergillus niger for galactaric acid production: elimination of galactaric acid catabolism by using RNA sequencing and CRISPR/Cas9

Production of Adipic Acid from Sugar Beet Residue by Combined Biological and Chemical Catalysis

Contact Info

Product

Resources

About