Conversion of Milled Pine Wood by Manganese Peroxidase from
            <i>Phlebia radiata</i>

Hofrichter, Martin; Lundell, Taina; Hatakka, Annele

doi:10.1128/aem.67.10.4588-4593.2001

Cited by 103 publications

(62 citation statements)

References 43 publications

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“…The resulting chelator deficiency could be responsible for the slowing down of the BaP conversion. A similar phenomenon has been observed for different organic acids during the in vitro conversion of milled pinewood by MnP from Phlebia radiata (28) and has been proposed as a source of H 2 O 2 in cultures of Ceriporiopsis subvermispora (51).…”

Section: Conversion Of Bap By Whole Fungal Cultures S Coronilla Consupporting

confidence: 75%

Degradation of Benzo[ a ]pyrene by the Litter-Decomposing Basidiomycete Stropharia coronilla : Role of Manganese Peroxidase

Steffen

Hatakka

Hofrichter

2003

Appl Environ Microbiol

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The litter-decomposing basidiomycete Stropharia coronilla, which preferably colonizes grasslands, was found to be capable of metabolizing and mineralizing benzo Benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon (PAH) consisting of five fused benzene rings, is known to be carcinogenic and mutagenic (10,12). Together with other PAHs, BaP is commonly formed during the pyrolysis and incomplete combustion of biological material and organic compounds and is found in various concentrations in coal tar, petroleum, and oil-based fuels (42). Thus, soils from gasworks sites and carbochemical plants as well as refineries and filling stations are often contaminated with BaP. This causes an obvious health risk that has raised the public interest in the fate and the removal of BaP and similar compounds in and from the environment.PAHs with more than four rings are considered highly recalcitrant and resistant to microbial degradation (10). In addition, the higher their molecular weight is, the lower is their water solubility and thus their bioavailability (54). While lowermolecular-mass PAHs-e.g., naphthalene, anthracene, and phenanthrene-are readily degraded by a number of aerobic bacteria, which can utilize these compounds as carbon sources (10,12,33), BaP and other PAHs with high molecular masses are cometabolically degraded by only a few bacterial species (Mycobacterium spp. and Sphingomonas spp.) and mixed microbial cultures (33).In addition to certain bacteria, wood-colonizing basidiomycetes such as Phanerochaete chrysosporium and Bjerkandera sp. strain BOS55 have been shown to metabolize BaP in liquid culture and soil (9, 24, 36). These white-rot fungi produce extracellular ligninolytic oxidoreductases, namely, manganese peroxidase (MnP), lignin peroxidase (LiP), and laccase, which attack aromatic substances, including PAHs, via the formation of free radicals (25,26,35). Since these fungi specialize in colonizing compact wood (timber and stumps) and cannot compete in soil for a prolonged time, their actual contribution to the removal of recalcitrant PAHs under natural conditions seems to be limited. There is, however, a second ecophysiological group of ligninolytic basidiomycetes-the litter-decomposing fungi-which have recently been shown to possess a ligninolytic enzyme system similar to that of white-rot fungi (48,49). Furthermore, screening tests have demonstrated that litter-decomposing fungi are capable of metabolizing PAHs, including BaP, to some extent (21,22,47,56).In the present study, we report the degradation of BaP by the litter-decomposing fungus Stropharia coronilla, which preferably inhabits grasslands. The species is a typical grass dweller that colonizes pastures, meadows, and waysides and is found in both Europe and North America (8,40). Particular attention is paid to the role of MnP, the predominant ligninolytic enzyme of this fungus, and the results indicate its crucial role in the degradation of PAH. MATERIALS AND METHODSOrganism, culture conditions, and enzyme preparation. The litter-decompos...

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Section: Conversion Of Bap By Whole Fungal Cultures S Coronilla Consupporting

confidence: 75%

Degradation of Benzo[ a ]pyrene by the Litter-Decomposing Basidiomycete Stropharia coronilla : Role of Manganese Peroxidase

Steffen

Hatakka

Hofrichter

2003

Appl Environ Microbiol

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“…An HPSEC method described previously (19,22) was used for the determination of the molecular mass distribution of humic substances. Sodium polystyrene sulfonates (1.3 to 168 kDa) and biphenyl dicarboxylic acid (0.246 kDa) served as molecular mass standards.…”

Section: Methodsmentioning

confidence: 99%

Degradation of Humic Acids by the Litter-Decomposing Basidiomycete Collybia dryophila

Steffen

Hatakka

Hofrichter

2002

Appl Environ Microbiol

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141

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“…This finding agrees with the data of Nagy et al (2012) who showed that the rate of selective delignification of RZ by H. parviporum is slow compared to that of heartwood. In vitro, the short-MnP3 from P. radiata was able to oxidize and convert phenolic and aromatic compounds dissolved from milled pine wood, and the process was enhanced in the presence of lipids and Mn 2+ ions (Hofrichter et al, 2001). The enzyme also demonstrates some Mn-independent peroxidation activity against phenolic compounds and amines, thus catalytically resembling versatile peroxidases (VP) (Hatakka et al, 2003;Heinfling et al, 1998).…”

Section: Transcript Regulation Of Ligninolytic Genes During Colonizatmentioning

confidence: 99%

Genes associated with lignin degradation in the polyphagous white-rot pathogen Heterobasidion irregulare show substrate-specific regulation

Yakovlev¹,

Hietala²,

Courty

et al. 2013

Fungal Genetics and Biology

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Conversion of Milled Pine Wood by Manganese Peroxidase from Phlebia radiata

Cited by 103 publications

References 43 publications

Degradation of Benzo[ a ]pyrene by the Litter-Decomposing Basidiomycete Stropharia coronilla : Role of Manganese Peroxidase

Degradation of Benzo[ a ]pyrene by the Litter-Decomposing Basidiomycete Stropharia coronilla : Role of Manganese Peroxidase

Degradation of Humic Acids by the Litter-Decomposing Basidiomycete Collybia dryophila

Genes associated with lignin degradation in the polyphagous white-rot pathogen Heterobasidion irregulare show substrate-specific regulation

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