Conversion of glycerol to 1,3-propanediol by Citrobacter freundii and Hafnia alvei – newly isolated strains from the Enterobacteriaceae

Drożdżyńska, Agnieszka; Pawlicka, Joanna; Kubiak, Piotr; Kośmider, Alicja; Pranke, Dorota; Olejnik-Schmidt, Agnieszka; Czaczyk, Katarzyna

doi:10.1016/j.nbt.2014.04.002

Cited by 47 publications

(30 citation statements)

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“…Fermentative production of 1,3‐PDO by microorganisms has been extensively studied and achieved success in batch, fed‐batch, repeated batch, and continuous fermentative systems in the past decades. Many species of microbes including the Clostridium, Klebsiella, Citrobacter, Enterobacter and even Shimwellia can convert crude glycerol to 1,3‐PDO. Among these microbes C. butyricum is thought to be one of the most prominent 1,3‐PDO producers owing to its exceptional and efficient performance in 1,3‐PDO production.…”

Section: Introductionmentioning

confidence: 99%

Adaptive evolution of Clostridium butyricum and scale‐Up for high‐Concentration 1,3‐propanediol production

et al. 2018

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In order to obtain the excellent 1,3-propanediol (1,3-PDO) producer from wild-type Clostridium butyricum, adaptive evolution was carried out to select the strain for fast growth. The most significant change was that fermentation time decreased from 36 h to 20 h after adaptive evolution. Thus, it led to the corresponding volumetric productivity of 1,3-PDO increasing from 0.97 to 2.14 (g/LÁh −1 ) which increased by 114%. Adaptive evolution was also applied to butyric acid tolerant strain selected based on the fast-growing one through a simple equipment. 1,3-PDO concentration increased from 40.28 to 66.23 g/L through fed-batch fermentation by butyric acid tolerant strain compared with the fast-growing one. In addition, the endpoint strain was successfully and steadily used in the 50-L scale fermentation. Thus, adaptive evolution is an excellent strategy which can help us select the fast-growing strain and reduce the negative effect from substrate and metabolite inhibition. (a) the morphology of Clostridium butyricum in the initial stage of Gen 0 adaptive evolution process. (b) The morphology of Clostridium butyricum in the last stage of Gen 0 adaptive evolution process. (c) The effect of 5 g/L butyric acid and acetic acid to the growth of Gen 4 strain. (d) Different growing status of Gen 0, Gen 1, Gen 2, Gen 3, and Gen 4 strain in seed culture contained 70 g/L glycerol. The "XMU" were written on the back of the each bottles. [Color figure can be viewed at wileyonlinelibrary.com] Figure 5. Time course of Gen 7 strain fermentation on 50-L scale. (a, b) Represent the concentrations of glycerol, 1,3-PDO (g/L), acids and OD in batch and fed-batch fermentation process.[Color figure can be viewed at wileyonlinelibrary.com]

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Section: Introductionmentioning

confidence: 99%

Adaptive evolution of Clostridium butyricum and scale‐Up for high‐Concentration 1,3‐propanediol production

et al. 2018

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“…It is significant that a substrate for technologies utilizing microorganisms may be crude glycerol, a byproduct of biodiesel production. The biotechnological methods that have been designed to obtain 1,3-PD by converting glycerol use the bacteria of the genera Clostridium, Klebsiella, Citrobacter, Hafnia, Lactobacillus [6,7,8]. In the process of selecting a 1,3-PD producer, consideration must be given not only to such vital features as resistance to osmotic, toxic, and mechanical stresses or maintenance of growth capability under the conditions of industrial-scale synthesis but also to an appropriate cultivation method [9,10].…”

Section: Introductionmentioning

confidence: 99%

1,3-Propanediol production from crude glycerol by Clostridium butyricum DSP1 in repeated batch

Szymanowska

2014

Electronic Journal of Biotechnology

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Background: The production of biofuels from renewable energy sources is one of the most important issues in industrial biotechnology today. The process is known to generate various by-products, for example crude glycerol, which is obtained in the making of biodiesel from rapeseed oil. Crude glycerol may be utilized in many ways, including microbial conversion to 1,3-propanediol (1,3-PD), a raw material for the synthesis of polyesters and polyurethanes. Results: The paper presents results of a study on the synthesis of 1,3-propanediol from crude glycerol by a repeated batch method with the use of Clostridium butyricum DSP1. Three cycles of fermentation medium replacement were carried out. The final concentration of 1,3-PD was 62 g/L and the maximum productivity, obtained during the second cycle, reached 1.68 g/L/h. Additionally, experiments conducted in parallel to the above involved using the entire quantity of the culture broth removed from the bioreactor to inoculate successive portions of fermentation media containing crude glycerol at concentrations of 80 g/L and 100 g/L. Under those conditions, the maximum 1,3-PD concentrations were 43.2 g/L and 54.2 g/L. Conclusions: The experiments proved that by using a portion of metabolically active biomass as inoculum for another fermentation formula it is possible to eliminate the stage of inoculum growth and thereby reduce the length of the whole operation. Additionally, that strategy avoids the phase of microbial adaptation to a different source of carbon such as crude glycerol, which is more difficult to utilize, thus improving the kinetic parameters of 1,3-PD production.

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“…However, Klebsiella spp belong to a second hazard group which limits their application in biotechnological processes. Some other representatives of Enterobacteriaceae were also reported to produce 1,3-PD at considerable amounts, like Citrobacter freundii (Homann et al, 1990;Metsoviti et al, 2013), Enterobacter agglomerans (Barbirato et al, 1995), Shimwellia blattae (Rodriguez et al, 2016) and recently also Hafnia alvei (Drożdżyńska et al, 2014). The latter species stayed within the focus of this study.…”

Section: Introductionmentioning

confidence: 64%

“…As indicated by Cui et al (2014), high lactate production restrains sufficient pool of reducing equivalents, available for 1,3-PD dh, imposing constraints on both cellular growth and 1,3-PD formation. Our previous study demonstrated that lactate was the major by-product formed during glycerol fermentation in H. alvei AD27 cultures (Drożdżyńska et al, 2014). At higher glycerol concentrations (up to 50 gL -1 ) accumulation of lactate was even higher than of 1,3-PD.…”

Section: Introductionmentioning

confidence: 88%

“…To this end we applied an algorithm-designed retrohomed group II intron for targeted, site-specific disruption of lactate dehydrogenase gene, ldh, in this strain. The obtained recombinants were screened for lactate production and further subjected to cultivation tests in pre-optimized medium (Drożdżyńska et al, 2014). Additional insight into the molecular background of the observed modification was provided by flow-cytometry analyses.…”

Section: Introductionmentioning

confidence: 99%

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Group II intron-mediated deletion of lactate dehydrogenase gene in an isolated 1,3-propanediol producer Hafnia alvei AD27

Celińska

Drożdżyńska²,

Wita³

et al. 2017

Acta Biochim Pol

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Our previous studies showed that glycerol fermentation by Hafnia alvei AD27 strain was accompanied by formation of high quantities of lactate. The ultimate aim of this work was the elimination of excessive lactate production in the 1,3-propanediol producer cultures. Group II intron-mediated deletion of ldh (lactate dehydrogenase) gene in an environmental isolate of H. alvei AD27 strain was conducted. The effect of the Δldh genotype in H. alvei AD27 strain varied depending on the culture medium applied. Under lower initial glycerol concentration (20 gL), lactate and 1,3-propanediol production was fully abolished, and the main carbon flux was directed to ethanol synthesis. On the other hand, at higher initial glycerol concentrations (40 gL), 1,3-propanediol and lactate production was recovered in the recombinant strain. The final titers of 1,3-propanediol and ethanol were similar for the recombinant and the WT strains, while the Δldh genotype displayed significantly decreased lactate titer. The by-products profile was altered upon ldh gene deletion, while glycerol utilization and biomass accumulation remained unaltered. As indicated by flow-cytometry analyses, the internal pH was not different for the WT and the recombinant Δldh strains over the culture duration, however, the WT strain was characterized by higher redox potential.

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Conversion of glycerol to 1,3-propanediol by Citrobacter freundii and Hafnia alvei – newly isolated strains from the Enterobacteriaceae

Cited by 47 publications

References 33 publications

Adaptive evolution of Clostridium butyricum and scale‐Up for high‐Concentration 1,3‐propanediol production

Adaptive evolution of Clostridium butyricum and scale‐Up for high‐Concentration 1,3‐propanediol production

1,3-Propanediol production from crude glycerol by Clostridium butyricum DSP1 in repeated batch

Group II intron-mediated deletion of lactate dehydrogenase gene in an isolated 1,3-propanediol producer Hafnia alvei AD27

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