Conversion of furfural in aerobic and anaerobic batch fermentation of glucose by Saccharomyces cerevisiae

Taherzadeh, Mohammad J.; Gustafsson, Lena; Niklasson, Claes; Lidén, Gunnar

doi:10.1016/s1389-1723(99)89007-0

Cited by 231 publications

(184 citation statements)

References 16 publications

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“…Silencing of this enzyme increased not only tolerance to furfural but also to the closely-related 5-hydroxymethyl furfural by increasing NADPH availability for biosynthesis (Miller et al 2010). Similar effects were observed in yeast, where furfural and 5-hydroxymethyl furfural were both consumed during the period of growth inhibition (Taherzadeh et al 1999(Taherzadeh et al , 2000. Thus, one possible method of dealing with the toxic aldehydes present in bio-oil is to mitigate NADPH depletion by either silencing the respective aldehyde reductase or by a general increase in NADPH availability.…”

Section: Model Contaminantsmentioning

confidence: 54%

Hybrid thermochemical processing: fermentation of pyrolysis-derived bio-oil

Jarboe

Wen

Choi

et al. 2011

Appl Microbiol Biotechnol

104

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Thermochemical processing of biomass by fast pyrolysis provides a nonenzymatic route for depolymerization of biomass into sugars that can be used for the biological production of fuels and chemicals. Fermentative utilization of this bio-oil faces two formidable challenges. First is the fact that most bio-oil-associated sugars are present in the anhydrous form. Metabolic engineering has enabled utilization of the main anhydrosugar, levoglucosan, in workhorse biocatalysts. The second challenge is the fact that bio-oil is rich in microbial inhibitors. Collection of bio-oil in distinct fractions, detoxification of bio-oil prior to fermentation, and increased robustness of the biocatalyst have all proven effective methods for addressing this inhibition. AbstractThermochemical processing of biomass by fast pyrolysis provides a non-enzymatic route for depolymerization of biomass into sugars that can be used for the biological production of fuels and chemicals. Fermentative utilization of this bio-oil faces two formidable challenges. First is the fact that most bio-oil-associated sugars are present in the anhydrous form. Metabolic engineering has enabled utilization of the main anhydrosugar, levoglucosan, in workhorse biocatalysts. The second challenge is the fact that bio-oil is rich in microbial inhibitors. Collection of bio-oil in distinct fractions, detoxification of bio-oil prior to fermentation and increased robustness of the biocatalyst have all proven effective methods for addressing this inhibition.

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Section: Model Contaminantsmentioning

confidence: 54%

Hybrid thermochemical processing: fermentation of pyrolysis-derived bio-oil

Jarboe

Wen

Choi

et al. 2011

Appl Microbiol Biotechnol

104

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“…BA and HA productivity from hydrolysates decreased compared to pure glucose fermentations since the biomass hydrolysates also contain other inhibitors (e.g., furfural, 5-hydroxymethylfurfural) that the bacterium likely needs to detoxify. The detoxification of furfural to furfuryl-alcohol has been demonstrated in other organisms while growing in anaerobic conditions [43][44][45]. Another interesting observation was that the HA:BA ratio increased in hydrolysates (1.73 for DDA and 2.0 for DMR, respectively) compared to glucose (1.54).…”

Section: Discussionmentioning

confidence: 83%

Mixed Carboxylic Acid Production by Megasphaera elsdenii from Glucose and Lignocellulosic Hydrolysate

et al. 2017

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Volatile fatty acids (VFAs) can be readily produced from many anaerobic microbes and subsequently utilized as precursors to renewable biofuels and biochemicals. Megasphaera elsdenii represents a promising host for production of VFAs, butyric acid (BA) and hexanoic acid (HA). However, due to the toxicity of these acids, product removal via an extractive fermentation system is required to achieve high titers and productivities. Here, we examine multiple aspects of extractive separations to produce BA and HA from glucose and lignocellulosic hydrolysate with M. elsdenii. A mixture of oleyl alcohol and 10% (v/v) trioctylamine was selected as an extraction solvent due to its insignificant inhibitory effect on the bacteria. Batch extractive fermentations were conducted in the pH range of 5.0 to 6.5 to select the best cell growth rate and extraction efficiency combination. Subsequently, fed-batch pertractive fermentations were run over 230 h, demonstrating high BA and HA concentrations in the extracted fraction (57.2 g/L from~190 g/L glucose) and productivity (0.26 g/L/h). To our knowledge, these are the highest combined acid titers and productivity values reported for M. elsdenii and bacterial mono-cultures from sugars. Lastly, the production of BA and HA (up to 17 g/L) from lignocellulosic sugars was demonstrated.

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“…In general, furans have been implicated in a broad range of effects, including inhibition of cell growth and glucose utilization, reduction of enzymic activity and ethanol productivity, DNA damage and inhibition of protein and RNA synthesis (as cited in Liu et al 2004). However, S. cerevisiae is capable of reducing furfural and 5-HMF to the less toxic furfuryl (Taherzadeh et al 1999) and 5-hydroxymethyl furfuryl alcohols (Taherzadeh et al 2000), respectively. When present alone, furfural is converted so efficiently that inhibitory effects decrease with increasing cell mass due to its rapid bioconversion (Navarro 1994).…”

Section: Common Inhibitors In Hydrolysates Effects and Mechanismsmentioning

confidence: 99%

Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: current status

Maris

Abbott

Bellissimi

et al. 2006

Antonie van Leeuwenhoek

432

276

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Fuel ethanol production from plant biomass hydrolysates by Saccharomyces cerevisiae is of great economic and environmental significance. This paper reviews the current status with respect to alcoholic fermentation of the main plant biomass-derived monosaccharides by this yeast. Wild-type S. cerevisiae strains readily ferment glucose, mannose and fructose via the Embden-Meyerhof pathway of glycolysis, while galactose is fermented via the Leloir pathway. Construction of yeast strains that efficiently convert other potentially fermentable substrates in plant biomass hydrolysates into ethanol is a major challenge in metabolic engineering. The most abundant of these compounds is xylose. Recent metabolic and evolutionary engineering studies on S. cerevisiae strains that express a fungal xylose isomerase have enabled the rapid and efficient anaerobic fermentation of this pentose.L-Arabinose fermentation, based on the expression of a prokaryotic pathway in S. cerevisiae, has also been established, but needs further optimization before it can be considered for industrial implementation. In addition to these already investigated strategies, possible approaches for metabolic engineering of galacturonic acid and rhamnose fermentation by S. cerevisiae are discussed. An emerging and major challenge is to achieve the rapid transition from proof-of-principle experiments under 'academic' conditions (synthetic media, single substrates or simple substrate mixtures, absence of toxic inhibitors) towards efficient conversion of complex industrial substrate mixtures that contain synergistically acting inhibitors.

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Conversion of furfural in aerobic and anaerobic batch fermentation of glucose by Saccharomyces cerevisiae

Cited by 231 publications

References 16 publications

Hybrid thermochemical processing: fermentation of pyrolysis-derived bio-oil

Hybrid thermochemical processing: fermentation of pyrolysis-derived bio-oil

Mixed Carboxylic Acid Production by Megasphaera elsdenii from Glucose and Lignocellulosic Hydrolysate

Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: current status

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