Conversion of Fishery Waste to Proteases by Streptomyces speibonae and Their Application in Antioxidant Preparation

Abstract

Proteinaceous wastes from the fishery process are an abundant renewable resource for the recovery of a variety of high-value products. This work attempted to utilize several proteinaceous wastes to produce proteases using the Streptomyces speibonae TKU048 strain. Among different possible carbon and nitrogen sources, the protease productive activity of S. speibonae TKU048 was optimal on 1% tuna head powder. Further, the casein/gelatin/tuna head powder zymography of the crude enzyme revealed the presence of three/nine/six proteases, respectively. The crude-enzyme cocktail of S. speibonae TKU048 exhibited the best proteolytic activity at 70 °C and pH = 5.8. Sodium dodecyl sulfate strongly enhanced the proteolytic activity of the cocktail, whereas FeCl3, CuSO4, and ethylenediaminetetraacetic acid could completely inhibit the enzyme activity. Additionally, the crude-enzyme cocktail of S. speibonae TKU048 could efficiently enhance the 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activities of all tested proteinaceous materials including the head, viscera, and meat of tuna fish; the head, viscera, and meat of tilapia fish; the head, meat, and shell of shrimp; squid pen; crab shell; and soybean. Taken together, S. speibonae TKU048 revealed potential in the reclamation of proteinaceous wastes for protease production and antioxidant preparation.

Purification and Characterization of a Small Thermostable Protease from Streptomyces sp. CNXK100

“…It exhibited similarities to proteases from other Streptomyces strains, including S. koyangensis TN650 ( Ben Elhoul et al 2015 ), Streptomyces sp. LCJ12A ( Parthasarathy and Gnanadoss 2020 ), and S. speibonae ( Tran et al 2022 ). Consequently, this protease could be employed to produce peptones and peptides from casein ( Rao et al 2020 ).…”

Optimization Production of an Endo-β-1,4-Xylanase from Streptomyces thermocarboxydus Using Wheat Bran as Sole Carbon Source

“…This species was reported for its capacity to generate thermostable xylanases that remain active at elevated temperatures (≥60 • C) [14,15]. Streptomyces strains also exhibit the capability to utilize a diverse array of carbon and nitrogen sources, including various types of residues, for the biosynthesis of enzymes [16][17][18][19][20], including xylanase [21][22][23].…”