Conversion between porcine naïve-like and primed ESCs and specific pluripotency marker identification

Chen, Qiaoyu; Zhang, Hong; Jiang, Haibin; Zhang, Manling; Wang, Junzheng; Zhao, Lihua; Wang, Chenyu; Liu, Manling; Li, Rongfeng

doi:10.1007/s11626-020-00448-3

Cited by 7 publications

(3 citation statements)

References 40 publications

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“…Our lab also previously derived porcine ESCs in the primed state. The primed ESCs had a stable phenotype and a normally karyotype, and they expressed pluripotency genes as well as some lineage-associated genes and could form EBs in vitro [ 15 , 32 ]. We found that the pXEN cells had a similar morphology to that of primed-state ESCs, so we wondered whether pXEN cells might be molecularly identical to primed-state ES cells.…”

Section: Resultsmentioning

confidence: 99%

“…In the present study, we demonstrated that the proliferation of XEN cells is dependent on the FGF and TGFβ signaling pathways. The conversion between porcine naïve-like and primed ESCs had be realized quickly via down-regulation of the LIF signaling pathway and up-regulation of the FGF [ 32 ]. Porcine naïve-like ESCs also can exit pluripotency and transition to the XEN state.…”

Section: Discussionmentioning

confidence: 99%

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Derivation of Porcine Extra-Embryonic Endoderm Cell Lines Reveals Distinct Signaling Pathway and Multipotency States

Zhang

Jin

Zhao

et al. 2021

IJMS

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The inner cell mass of the pre-implantation blastocyst consists of the epiblast and hypoblast from which embryonic stem cells (ESCs) and extra-embryonic endoderm (XEN) stem cells, respectively, can be derived. Importantly, each stem cell type retains the defining properties and lineage restriction of its in vivo tissue origin. We have developed a novel approach for deriving porcine XEN (pXEN) cells via culturing the blastocysts with a chemical cocktail culture system. The pXEN cells were positive for XEN markers, including Gata4, Gata6, Sox17, and Sall4, but not for pluripotent markers Oct4, Sox2, and Nanog. The pXEN cells also retained the ability to undergo visceral endoderm (VE) and parietal endoderm (PE) differentiation in vitro. The maintenance of pXEN required FGF/MEK+TGFβ signaling pathways. The pXEN cells showed a stable phenotype through more than 50 passages in culture and could be established repeatedly from blastocysts or converted from the naïve-like ESCs established in our lab. These cells provide a new tool for exploring the pathways of porcine embryo development and differentiation and providing further reference to the establishment of porcine ESCs with potency of germline chimerism and gamete development.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Derivation of Porcine Extra-Embryonic Endoderm Cell Lines Reveals Distinct Signaling Pathway and Multipotency States

Zhang

Jin

Zhao

et al. 2021

IJMS

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“…At p5, LIN28B is also significantly downregulated. Interestingly, LIN28 has been recently identified as a naïve-specific marker in porcine ESCs (Chen et al, 2020), at both the RNA and protein level, suggesting that its downregulation may be somewhat detrimental also in naïve hESCs.…”

Section: Discussionmentioning

confidence: 99%

KLF17 promotes human naïve pluripotency but is not required for its establishment

Lea

McCarthy

Boeing

et al. 2020

Preprint

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Current knowledge of the transcriptional regulation of human pluripotency is incomplete, with lack of inter-species conservation observed. Single-cell transcriptomics of human embryos previously enabled us to identify transcription factors, including the zinc-finger protein KLF17, that are enriched in the human epiblast and naïve hESCs. Here we show that KLF17 is expressed coincident with the known pluripotency factors NANOG and SOX2 across human blastocyst development. We investigate the function of KLF17 in pluripotency using primed and naïve hESCs for gain- and loss-of-function analyses. We find that ectopic expression of KLF17 in primed hESCs is sufficient to induce a naïve-like transcriptome and that KLF17 can drive transgene-mediated resetting to naïve pluripotency. This implies a role for KLF17 in establishing naïve pluripotency. However, CRISPR-Cas9-mediated knockout studies reveal that KLF17 is not required for naïve pluripotency acquisition in vitro. Transcriptome analysis of naïve hESCs identifies subtle effects on metabolism and signalling following KLF17 loss of function, and possible redundancy with the related factor, KLF5. Overall, we show that KLF17 is sufficient, but not necessary, for naïve pluripotency under the given in vitro conditions.Summary statementInvestigating KLF17 in human pluripotency reveals that it is sufficient, but not necessary, to establish naïve hESCs. We posit that KLF17 is a peripheral regulator, like KLF2 in the mouse.

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Label-free and non-destructive identification of naïve and primed embryonic stem cells based on differences in cellular metabolism

Koo¹,

Go²,

Kim³

et al. 2023

Biomaterials

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Conversion between porcine naïve-like and primed ESCs and specific pluripotency marker identification

Cited by 7 publications

References 40 publications

Derivation of Porcine Extra-Embryonic Endoderm Cell Lines Reveals Distinct Signaling Pathway and Multipotency States

Derivation of Porcine Extra-Embryonic Endoderm Cell Lines Reveals Distinct Signaling Pathway and Multipotency States

KLF17 promotes human naïve pluripotency but is not required for its establishment

Label-free and non-destructive identification of naïve and primed embryonic stem cells based on differences in cellular metabolism

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