Convergent reductive evolution and host adaptation in Mycoavidus bacterial endosymbionts of Mortierellaceae fungi

Amses, Kevin; Desiró, Alessandro; Bryson, Abigail; Grigoriev, Igor; Mondo, Stephen; Lipzen, Anna; LaButti, Kurt; Riley, Robert; Singan, Vasanth; Salazar-Hamm, Paris; King, Jason; Ballou, Elizabeth; Pawlowska, Teresa; Adeleke, Rasheed; Bonito, Gregory; Uehling, Jessie

doi:10.1016/j.fgb.2023.103838

Cited by 4 publications

(2 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our phylogenetic analysis of these 90 OTUs showed two MRE clusters: MRE I with 20 OTUs from this study, and MRE II with 47. Remarkably, the OTUs associated with BRE did not group with any of the groups BRE A, B and C previously reported (Amses et al, 2023 ; Okrasinska et al, 2021 ; Takashima et al, 2018 ) (Figure 3 , Table S13 ). We recovered two OTUs that are similar to the Candidatus Glomeribacter group and only present in the spores, and two OTUs within Burkholderia sensu lato.…”

Section: Resultsmentioning

confidence: 60%

Desert plants, arbuscular mycorrhizal fungi and associated bacteria: Exploring the diversity and role of symbiosis under drought

Chávez‐González,

Flores‐Núñez,

Merino‐Espinoza

et al. 2024

Environ Microbiol Rep

View full text Add to dashboard Cite

Desert plants, such as Agave tequilana, A. salmiana and Myrtillocactus geometrizans, can survive harsh environmental conditions partly due to their symbiotic relationships with microorganisms, including arbuscular mycorrhizal fungi (AMF). Interestingly, some of these fungi also harbour endosymbiotic bacteria. Our research focused on investigating the diversity of these AMFs and their associated bacteria in these plants growing in arid soil. We found that agaves have a threefold higher AMF colonization than M. geometrizans. Metabarcoding techniques revealed that the composition of AMF communities was primarily influenced by the plant host, while the bacterial communities were more affected by the specific plant compartment or niche they inhabited. We identified both known and novel endofungal bacterial taxa, including Burkholderiales, and confirmed their presence within AMF spores using multiphoton microscopy. Our study also explored the effects of drought on the symbiosis between A. tequilana and AMF. We discovered that the severity of drought conditions could modulate the strength of this symbiosis and its outcomes for the plant holobiont. Severe drought conditions prevented the formation of this symbiosis, while moderate drought conditions promoted it, thereby conferring drought tolerance in A. tequilana. This research sheds light on the diversity of AMF and associated bacteria in Crassulacean Acid Metabolism (CAM) plants and underscores the crucial role of drought as a factor modulating the symbiosis between A. tequilana and AMF. Further research is needed to understand the role of endofungal bacteria in this response.

show abstract

Section: Resultsmentioning

confidence: 60%

Desert plants, arbuscular mycorrhizal fungi and associated bacteria: Exploring the diversity and role of symbiosis under drought

Chávez‐González,

Flores‐Núñez,

Merino‐Espinoza

et al. 2024

Environ Microbiol Rep

View full text Add to dashboard Cite

show abstract

“…analogous to recent studies with endofungal Mycoavidus spp. [ 58 ] was outside the scope of this study given the limited number of high-quality assemblies, identifying and sequencing additional strains would enable a deeper investigation into the Mycetohabitans pangenome, shedding more light on the evolutionary trends within and between the species themselves that will inform hypotheses of effector evolution.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and diversity of TAL effector-like proteins in fungal endosymbiotic Mycetohabitans spp.

Carpenter,

Bogdanove,

Abbot

et al. 2024

Microbial Genomics

Self Cite

View full text Add to dashboard Cite

Endofungal Mycetohabitans (formerly Burkholderia) spp. rely on a type III secretion system to deliver mostly unidentified effector proteins when colonizing their host fungus, Rhizopus microsporus. The one known secreted effector family from Mycetohabitans consists of homologues of transcription activator-like (TAL) effectors, which are used by plant pathogenic Xanthomonas and Ralstonia spp. to activate host genes that promote disease. These ‘Burkholderia TAL-like (Btl)’ proteins bind corresponding specific DNA sequences in a predictable manner, but their genomic target(s) and impact on transcription in the fungus are unknown. Recent phenotyping of Btl mutants of two Mycetohabitans strains revealed that the single Btl in one Mycetohabitans endofungorum strain enhances fungal membrane stress tolerance, while others in a Mycetohabitans rhizoxinica strain promote bacterial colonization of the fungus. The phenotypic diversity underscores the need to assess the sequence diversity and, given that sequence diversity translates to DNA targeting specificity, the functional diversity of Btl proteins. Using a dual approach to maximize capture of Btl protein sequences for our analysis, we sequenced and assembled nine Mycetohabitans spp. genomes using long-read PacBio technology and also mined available short-read Illumina fungal–bacterial metagenomes. We show that btl genes are present across diverse Mycetohabitans strains from Mucoromycota fungal hosts yet vary in sequences and predicted DNA binding specificity. Phylogenetic analysis revealed distinct clades of Btl proteins and suggested that Mycetohabitans might contain more species than previously recognized. Within our data set, Btl proteins were more conserved across M. rhizoxinica strains than across M. endofungorum, but there was also evidence of greater overall strain diversity within the latter clade. Overall, the results suggest that Btl proteins contribute to bacterial–fungal symbioses in myriad ways.

show abstract

Prevalence and diversity of TAL effector-like proteins in fungal endosymbiotic Mycetohabitans spp.

Carpenter,

Bogdanove,

Abbot

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Endofungal Mycetohabitans (formerly Burkholderia) spp. rely on a type III secretion system to deliver mostly unidentified effector proteins when colonizing their host fungus, Rhizopus microsporus. The oneknown secreted effector family from Mycetohabitans consists of homologs of transcription activator-like (TAL) effectors, which are used by plant pathogenic Xanthomonas and Ralstonia spp. to activate host genes that promote disease. These Burkholderia TAL-like (Btl) proteins bind corresponding specific DNA sequences in a predictable manner, but their impact on transcription and their genomic target(s) in the fungus are not yet known. Recent characterization of two Btl proteins (Btl19-13 and MTAL1/Btl21-1), each from a different Mycetohabitans species, revealed different phenotypes in Rhizopus, underscoring the need to assess the sequence and functional diversity of Btl proteins. We sequenced and assembled nine Mycetohabitans spp. genomes using long-read PacBio technology. All assemblies contained fragments of btl genes, and most had intact copies. We then mined fungal-bacterial metagenomes assembled as part of the ZygoLife project. This analysis showed that btl genes are present across diverse Mycetohabitans strains from Mucoromycota fungal hosts yet vary in sequences and predicted DNA binding specificity. Phylogenetic analysis revealed distinct clades of Btl proteins and suggested that Mycetohabitans might contain more species than previously recognized. Within our data set, Btl proteins were more conserved across Mycetohabitans rhizoxinica strains than across Mycetohabitans endofungorum, but there was also evidence of greater overall strain diversity within the latter clade. Overall, the results suggest that Btl proteins contribute to bacterial-fungal symbioses in myriad ways.

show abstract

Convergent reductive evolution and host adaptation in Mycoavidus bacterial endosymbionts of Mortierellaceae fungi

Cited by 4 publications

References 50 publications

Desert plants, arbuscular mycorrhizal fungi and associated bacteria: Exploring the diversity and role of symbiosis under drought

Desert plants, arbuscular mycorrhizal fungi and associated bacteria: Exploring the diversity and role of symbiosis under drought

Prevalence and diversity of TAL effector-like proteins in fungal endosymbiotic Mycetohabitans spp.

Prevalence and diversity of TAL effector-like proteins in fungal endosymbiotic Mycetohabitans spp.

Contact Info

Product

Resources

About