Convergence of Cortical and Sensory Driver Inputs on Single Thalamocortical Cells

Groh, Alexander; Bokor, Hajnalka; Mease, Rebecca A.; Plattner, Viktor M; Hangya, Balázs; Stroh, Albrecht; Deschênes, Martin; Acsády, László

doi:10.1093/cercor/bht173

Cited by 149 publications

(222 citation statements)

References 45 publications

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“…We performed the experiments in two thalamic nuclei, VB and Po, known to have distinct excitatory and inhibitory connectivity (Barthó et al, 2002;Bokor et al, 2005;Wanaverbecq et al, 2008;Groh et al, 2013). We found significant internuclear differences in response to the removal of synaptic GABAergic inhibition.…”

Section: Discussionmentioning

confidence: 89%

“…We attribute these differences to the distinct connectivity and activity pattern of the two nuclei (Hoogland et al, 1991;Groh et al, 2013). In case of VB cells, during the Up states of ketamine/xylazine induced slow oscillation, the intracellular activity is dominated by nRT-mediated rhythmic inhibition, whereas in case of Po cells fast rising EPSPs arising from layer 5 pyramidal cells determine Po activity under similar conditions (Groh et al, 2013). In agreement with these data, removal of synaptic inhibition affected VB cells to a much greater extent than Po cells.…”

Section: Discussionmentioning

confidence: 99%

“…77I lox mice as previously described (Franken et al, 1998). Two weeks before surgery, 4 animals were bilaterally injected with AAV-GFP (as controls) or AAV-Cre.…”

Section: Methodsmentioning

confidence: 99%

“…GABA-ARs containing ␦ instead of ␥ subunits are abundant in extrasynaptic membranes (Nusser et al, 1998;Wei et al, 2003;Farrant and Nusser, 2005;Belelli et al, 2009), have higher sensitivity to GABA, and are activated by ambient GABA. These extrasynaptic receptors are primarily responsible for a slow, persistent (i.e., tonic) chloride current (Hamann et al, 2002;Wei et al, 2003;Cope et al, 2005;Bright et al, 2007Bright et al, , 2011.…”

Section: Introductionmentioning

confidence: 99%

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Phasic, Nonsynaptic GABA-A Receptor-Mediated Inhibition Entrains Thalamocortical Oscillations

et al. 2014

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GABA-A receptors (GABA-ARs) are typically expressed at synaptic or nonsynaptic sites mediating phasic and tonic inhibition, respectively. These two forms of inhibition conjointly control various network oscillations. To disentangle their roles in thalamocortical rhythms, we focally deleted synaptic, ␥2 subunit-containing GABA-ARs in the thalamus using viral intervention in mice. After successful removal of ␥2 subunit clusters, spontaneous and evoked GABAergic synaptic currents disappeared in thalamocortical cells when the presynaptic, reticular thalamic (nRT) neurons fired in tonic mode. However, when nRT cells fired in burst mode, slow phasic GABA-ARmediated events persisted, indicating a dynamic, burst-specific recruitment of nonsynaptic GABA-ARs. In vivo, removal of synaptic GABA-ARs reduced the firing of individual thalamocortical cells but did not abolish slow oscillations or sleep spindles. We conclude that nonsynaptic GABA-ARs are recruited in a phasic manner specifically during burst firing of nRT cells and provide sufficient GABA-AR activation to control major thalamocortical oscillations.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

“…77I lox mice as previously described (Franken et al, 1998). Two weeks before surgery, 4 animals were bilaterally injected with AAV-GFP (as controls) or AAV-Cre.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phasic, Nonsynaptic GABA-A Receptor-Mediated Inhibition Entrains Thalamocortical Oscillations

et al. 2014

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“…11B; Groh et al 2008Groh et al , 2014. This pathway is known to be extremely efficient at low stimulation frequencies but expresses pronounced short-term depression at higher frequencies.…”

Section: Large-scale Recordings From the Mouse Brain Combined With Opmentioning

confidence: 99%

Large-scale recording of thalamocortical circuits: in vivo electrophysiology with the two-dimensional electronic depth control silicon probe

Fiáth

Beregszászi

Horváth

et al. 2016

Journal of Neurophysiology

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Fiáth R, Beregszászi P, Horváth D, Wittner L, Aarts AA, Ruther P, Neves HP, Bokor H, Acsády L, Ulbert I. Large-scale recording of thalamocortical circuits: in vivo electrophysiology with the two-dimensional electronic depth control silicon probe. J Neurophysiol 116: 2312-2330. First published August 17, 2016 doi:10.1152/jn.00318.2016.-Recording simultaneous activity of a large number of neurons in distributed neuronal networks is crucial to understand higher order brain functions. We demonstrate the in vivo performance of a recently developed electrophysiological recording system comprising a two-dimensional, multi-shank, high-density silicon probe with integrated complementary metal-oxide semiconductor electronics. The system implements the concept of electronic depth control (EDC), which enables the electronic selection of a limited number of recording sites on each of the probe shafts. This innovative feature of the system permits simultaneous recording of local field potentials (LFP) and single-and multiple-unit activity (SUA and MUA, respectively) from multiple brain sites with high quality and without the actual physical movement of the probe. To evaluate the in vivo recording capabilities of the EDC probe, we recorded LFP, MUA, and SUA in acute experiments from cortical and thalamic brain areas of anesthetized rats and mice. The advantages of large-scale recording with the EDC probe are illustrated by investigating the spatiotemporal dynamics of pharmacologically induced thalamocortical slow-wave activity in rats and by the two-dimensional tonotopic mapping of the auditory thalamus. In mice, spatial distribution of thalamic responses to optogenetic stimulation of the neocortex was examined. Utilizing the benefits of the EDC system may result in a higher yield of useful data from a single experiment compared with traditional passive multielectrode arrays, and thus in the reduction of animals needed for a research study. electronic depth control; silicon probe; high-density recording; thalamocortical oscillation; optogenetics DESPITE THE RAPID ADVANCEMENT of brain imaging techniques offering both high spatial and temporal resolution, electrophysiological tools are still widely used methods to investigate the complex spatiotemporal activity patterns of neuronal circuits. Over the past few decades, single-wire electrodes used for in vivo extracellular recording of action potentials evolved into multielectrode arrays covering the range of up to 1,000 recording sites NEW & NOTEWORTHY Recording the electrical activity of a large number of neurons located in the thalamocortical

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Ultrastructural analysis of parvalbumin synapses in human dorsolateral prefrontal cortex

Glausier

Roberts

Lewis

2017

J of Comparative Neurology

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Coordinated activity of neural circuitry in the primate dorsolateral prefrontal cortex (DLPFC) supports a range of cognitive functions. Altered DLPFC activation is implicated in a number of human psychiatric and neurological illnesses. Proper DLPFC activity is, in part, maintained by two populations of neurons containing the calcium-binding protein parvalbumin (PV): local inhibitory interneurons that form Type II synapses, and long-range glutamatergic inputs from the thalamus that form Type I synapses. Understanding the contributions of each PV neuronal population to human DLPFC function requires a detailed examination of their anatomical properties. Consequently, we performed an electron microscopic analysis of 1) the distribution of PV immunoreactivity within the neuropil, 2) the properties of dendritic shafts of PV-IR interneurons, 3) Type II PV-IR synapses from PV interneurons, and 4) Type I PV-IR synapses from long-range projections, within the superficial and middle laminar zones of the human DLPFC. In both laminar zones, Type II PV-IR synapses from interneurons comprised ~60% of all PV-IR synapses, and Type I PV-IR synapses from putative thalamocortical terminals comprised the remaining ~40% of PV-IR synapses. Thus, the present study suggests that innervation from PV-containing thalamic nuclei extends across superficial and middle layers of the human DLPFC. These findings contrast with previous ultrastructural studies in monkey DLPFC where Type I PV-IR synapses were not identified in the superficial laminar zone. The presumptive added modulation of DLPFC circuitry by the thalamus in human may contribute to species-specific, higher-order functions.

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Convergence of Cortical and Sensory Driver Inputs on Single Thalamocortical Cells

Cited by 149 publications

References 45 publications

Phasic, Nonsynaptic GABA-A Receptor-Mediated Inhibition Entrains Thalamocortical Oscillations

Phasic, Nonsynaptic GABA-A Receptor-Mediated Inhibition Entrains Thalamocortical Oscillations

Large-scale recording of thalamocortical circuits: in vivo electrophysiology with the two-dimensional electronic depth control silicon probe

Ultrastructural analysis of parvalbumin synapses in human dorsolateral prefrontal cortex

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