Conventional Specimen Preparation Techniques for Transmission Electron Microscopy of Cultured Cells

Bozzola, John J.

doi:10.1007/978-1-62703-776-1_1

Cited by 57 publications

(66 citation statements)

References 6 publications

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“…A detailed protocol was followed as previously described by Bozzola with minimal modification (41). Ultrathin sections were post-stained and examined using a Hitachi transmission electron microscope.…”

Section: Methodsmentioning

confidence: 99%

Myosin Light Chain Kinase (MLCK) Regulates Cell Migration in a Myosin Regulatory Light Chain Phosphorylation-independent Mechanism

Chen

Tao

Wen

et al. 2014

Journal of Biological Chemistry

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Background: MLCK in cell migration remains controversial. Results: MLCK deletion causes enhanced cell protrusion along with a reduction of membrane tension and is rescued by kinase-dead MLCK or five-DFRXXL motif. Conclusion: MLCK regulates cell migration not by myosin regulatory light chain phosphorylation but possibly through a membrane tension-based mechanism. Significance: Our results shed light on a novel regulatory mechanism of protrusion during cell migration.

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Section: Methodsmentioning

confidence: 99%

Myosin Light Chain Kinase (MLCK) Regulates Cell Migration in a Myosin Regulatory Light Chain Phosphorylation-independent Mechanism

Chen

Tao

Wen

et al. 2014

Journal of Biological Chemistry

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“…The obtained data were analyzed using ANOVA and Post Hoc test. Significant P value was set at 0.05 (12).…”

Section: Quantitative Evaluation For the Immunostained Sectionsmentioning

confidence: 99%

Histological study of the possible protective action of omega-3-fatty acids on the injurious effect induced by Bisphenol A on rat hippocampus

Elaziz

Laag

2018

Egyptian Journal of Histology

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Introduction: Bisphenol A (BPA), a well-known industrial chemical, has adverse effects on the brain even at relatively low exposure levels in rodents, primates and humans. Omega-3-fatty acids participate in a number of neuronal processes including neurogenesis, neuron differentiation, and neuro-protection. Aim of the work: To investigate the possible protective action of omega-3-fatty acids on the injurious histological effect induced by Bisphenol A on rat hippocampus. Materials and Methods: Eighteen adult male Wistar rats were divided into 3 equal groups; control, BPA (1.2mg/kg daily for 3 weeks, intraperitoneally) and the third group were given omega-3 (300mg/kg orally) in addition to BPA in the aforementioned dose and duration. Hippocampus sections were processed for hematoxylin and eosin staining, GFAB staining and electron microscopic examination. Results: BBPA administration resulted into several histological alterations. Glial fibrillary acidic protein-positive cells were more abundant in the hippocampus of BPA-treated animals compared with the control animals. Ultra-structurally, the hippocampus of BPA-treated group showed nerve cells having nuclei with irregular outline and dilated perinuclear envelop, dilated RER and Golgi, swollen mitochondria with destroyed cristae. Some of the myelinated and unmyelinated nerve fibers showed degenerative changes. Concomitant administration of omega-3-fatty acids ameliorated these effects. Conclusions: OOmega-3-fatty acids partially minimized the severity of BPA-induced hippocampus injurious histological effects in Wistar rat.

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“…Strain typing was performed by pulsed-field gel electrophoresis (PFGE) as described by Mannu and Paba (2002). In addition, scanning electron microscopy (SEM) was performed on strains with a Zeiss DSM962 microscopy (Bozzola, 2007).…”

Section: Isolates Identification and Strain Typingmentioning

confidence: 99%

Sardinian goat’s milk as source of bacteriocinogenic potential protective cultures

et al. 2012

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Conventional Specimen Preparation Techniques for Transmission Electron Microscopy of Cultured Cells

Cited by 57 publications

References 6 publications

Myosin Light Chain Kinase (MLCK) Regulates Cell Migration in a Myosin Regulatory Light Chain Phosphorylation-independent Mechanism

Myosin Light Chain Kinase (MLCK) Regulates Cell Migration in a Myosin Regulatory Light Chain Phosphorylation-independent Mechanism

Histological study of the possible protective action of omega-3-fatty acids on the injurious effect induced by Bisphenol A on rat hippocampus

Sardinian goat’s milk as source of bacteriocinogenic potential protective cultures

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