Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Organelle interactions compartmentalize hepatic fatty acid trafficking and metabolism

“…To gain high-resolution insights into the dynamics and nanoscopic localization of single FAs as they pertain to mitochondrion utilization or storage in LDs, we applied single-molecule localization microscopy (SMLM) 34 with a recently developed approach using the fluorescently labeled FA analog BODIPY-C 12 in living cells. 35 , 36 SMLM offers the unique ability to determine the localization of single FAs and mitochondrion molecules in living cells with ~30-nm precision, enabling quantitative analysis of the localization density of one 37 or two 38 molecule species in a subcellular compartment. These SMLM experiments, therefore, fill the knowledge gap between static high-resolution electron microscopy (EM) studies and diffraction-limited conventional fluorescence microscopy and can quantify the density of FAs in different mitochondrial fractions with high precision.…”

Quantitative live-cell PALM reveals nanoscopic Faa4 redistributions and dynamics on lipid droplets during metabolic transitions of yeast

“…Likewise, it is not known how this relation changes upon metabolic shifts. Here we expand our recently developed live-cell single-molecule localization microscopy (SMLM) approach (Adhikari et al, 2019(Adhikari et al, , 2020 by resolving LDs at different metabolic states in living yeast cells. For the first time, we gain quantitative insights into the relation between the size of LDs and the density of the endogenously tagged fatty acid activation enzyme Faa4 on LDs upon metabolic transitions.…”