Conventional and unconventional extraction methods applied to the plant, <i>Thymus serpyllum</i> L

Đukić, Dragutin; Mašković, Pavle; Moračanin, S. V.; Kurčubić, Vladimir; Milijašević, Milan; Babić, Jurislav

doi:10.1088/1755-1315/85/1/012064

Cited by 12 publications

(10 citation statements)

References 15 publications

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“…Đukić et al evaluated various conventional (Soxhlet and macerate extraction) and non-conventional extraction (ultrasound, microwave and subcritical water extractions) methods from wild thyme. The results showed that the highest TPC and antioxidant activity was found using subcritical water extraction (141.12 ± 0.23 mg GAE/g and 170.32 ± 0.87 mg AA/G) [ 33 ]. Although these results seems higher compared to our study, no conclusions could be made regarding the best extraction method since different analytical methods were used and different solvents with different solvent to solid ratio were used.…”

Section: Discussionmentioning

confidence: 99%

High Voltage Electrical Discharges as an Alternative Extraction Process of Phenolic and Volatile Compounds from Wild Thyme (Thymus serpyllum L.): In Silico and Experimental Approaches for Solubility Assessment

et al. 2020

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The objective of this study was to evaluate the potential of green solvents for extractions of bioactive compounds (BACs) and essential oils from wild thyme (Thymus serpyllum L.) using theoretical and experimental procedures. Theoretical prediction was assessed by Hansen solubility parameters (HSPs) and conductor-like screening model for realistic solvents (COSMO-RS), to predict the most suitable solvents for extraction of BACs. An experimental procedure was performed by nonthermal technology high voltage electrical discharge (HVED) and it was compared with modified conventional extraction (CE). Obtained extracts were analyzed for chemical and physical changes during the treatment. Theoretical results for solution of BACs in ethanol and water, as green solvents, were confirmed by experimental results, while more accurate data was given by COSMO-RS assessment than HSPs. Results confirmed high potential of HVED for extraction of BACs and volatile compounds from wild thyme, in average, 2.03 times higher yield of extraction in terms of total phenolic content was found compared to CE. The main phenolic compound found in wild thyme extracts was rosmarinic acid, while the predominant volatile compound was carvacrol. Obtained extracts are considered safe and high-quality source reach in BACs that could be further used in functional food production.

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Section: Discussionmentioning

confidence: 99%

High Voltage Electrical Discharges as an Alternative Extraction Process of Phenolic and Volatile Compounds from Wild Thyme (Thymus serpyllum L.): In Silico and Experimental Approaches for Solubility Assessment

et al. 2020

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“…Extraction techniques represent an excellent way for valorization of the T. serpyllum plant material [ 5 , 11 , 12 , 13 ]. In order to overcome certain obstacles of conventional extraction techniques (maceration [ 14 ], heat-assisted extraction [ 5 ], percolation [ 15 ] and Soxhlet extraction [ 12 ]) in terms of shortening extraction time, reducing energy costs, reducing operating temperature to preserve thermolabile bioactive substances, using “green” solvents and obtaining extracts with maximized polyphenols yield and bioactivity, pressurized liquid extraction (PLE) was used in this study. PLE represents extraction procedure that uses organic solvents at high pressures and temperatures above the boiling point [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, PLE has an advantage over some extraction techniques like ultrasound- and microwave-assisted extractions in terms of no requirement for additional filtration step of crude extract, which makes the down-stream of the process much easier [ 18 ]. PLE has so far proved to be a very good approach for isolation of polyphenols from Lamiaceae species, such as mint ( Mentha piperita L.) [ 19 ], sage ( Salvia officinalis L.) [ 20 ], rosemary ( Rosmarinus officinalis L.) [ 21 ], thyme ( Thymus vulgaris L.) [ 22 ] and wild thyme ( T. serpyllum L.) [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Pressurized-Liquid Extraction as an Efficient Method for Valorization of Thymus serpyllum Herbal Dust towards Sustainable Production of Antioxidants

et al. 2021

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The aim of this study was to valorize Thymus serpyllum herbal dust, a particular fraction distinguished as an industrial waste from filter-tea production. Pressurized liquid extraction (PLE) was used with the aim of overcoming certain obstacles of conventional extraction techniques in terms of shortening extraction time, reducing solvent consumption and energy costs, using “green” solvents and obtaining high yield and quality products. In order to optimize PLE of T. serpyllum herbal dust, the preliminary screening of the independent variables in order to define the most influential parameters and their domain was done first. After the screening, the optimization study using the face-centered central composite experimental design (CCD) with response surface methodology (RSM) was implemented. Additionally, taking into account the high awareness of the positive influence of antioxidants on the human health and associating it with high content of polyphenolic compounds in various members of Lamiaceae family, PLE has proven to be a great approach for antioxidants recovery from T. serpyllum herbal dust.

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“…The extraction procedure is an important step in recovering bioactive compounds from plant matrices. Conventional (maceration, Soxhlet extraction) and non-conventional (ultrasound-assisted, microwave-assisted, accelerated solvent and subcritical water extraction techniques) approaches can be applied [ 3 ]. Extraction method effectiveness is evident when different methods are practiced on the same plant matrices by using the same solvent and extraction efficiency shows significant variations [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

New Insights into the Exploitation of Vitis vinifera L. cv. Aglianico Leaf Extracts for Nutraceutical Purposes

et al. 2020

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The leaves of Vitis vinifera L. have been used for a long time in traditional medicine for the treatment of many ailments. Grape polyphenols, indeed, have been demonstrated to be able to defend against oxidative stress, responsible for various disorders such as cancer, diabetes and neurodegenerative diseases. The effects of different extraction techniques, Soxhlet (SOX), Accelerated Solvent (ASE 40, ASE 50) and Ultrasound Assisted Extraction (UAE) were studied in this work to evaluate their impact on the chemical profile and bioactive potential of Vitis vinifera L. (cv. Aglianico) leaf extracts. The phytochemical profile was investigated by HPLC-DAD and 9 phenolic compounds were identified and quantified in the extract. Moreover, the antioxidant, anticholinesterase and antityrosinase activities were evaluated. In detail, the total polyphenol content and antioxidant activity (2,2-diphenyl-1-picrylhydrazyl, Oxygen Radical Absorbance Capacities and β-Carotene Bleaching assays) were evaluated and compared to assess the Relative Antioxidant Capacity Index (RACI). To test the inhibitory activity of extracts towards cholinesterases, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibition assays were performed. SOX and ASE 50 have shown the highest value of RACI, 0.76 and 0.65, respectively. Regarding enzymatic inhibitory activity, ASE 50 (IC50 = 107.16 ± 8.12 μg/mL) and SOX (IC50 = 171.34 ± 12.12 μg/mL) extracts exhibited the highest AChE and BChE inhibitory activity, respectively, while UAE (IC50 = 293.2 ± 25.6 μg/mL, followed by SOX (IC50 = 302.5 ± 38.3 μg/mL) showed the highest tyrosinase inhibition value. Our results demonstrated for the first time that Aglianico leaves are important sources of phenols that could be used to prevent oxidative stress and be potentially helpful in diseases treatable with tyrosinase and cholinesterase inhibitors, like myasthenia gravis or Alzheimer’s.

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Conventional and unconventional extraction methods applied to the plant, Thymus serpyllum L

Cited by 12 publications

References 15 publications

High Voltage Electrical Discharges as an Alternative Extraction Process of Phenolic and Volatile Compounds from Wild Thyme (Thymus serpyllum L.): In Silico and Experimental Approaches for Solubility Assessment

High Voltage Electrical Discharges as an Alternative Extraction Process of Phenolic and Volatile Compounds from Wild Thyme (Thymus serpyllum L.): In Silico and Experimental Approaches for Solubility Assessment

Pressurized-Liquid Extraction as an Efficient Method for Valorization of Thymus serpyllum Herbal Dust towards Sustainable Production of Antioxidants

New Insights into the Exploitation of Vitis vinifera L. cv. Aglianico Leaf Extracts for Nutraceutical Purposes

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