Conventional and molecular techniques for the early diagnosis of bacteraemia

Paolucci, Marina; Landini, Maria Paola; Sambri, Vittorio

doi:10.1016/j.ijantimicag.2010.11.010

Cited by 91 publications

(95 citation statements)

References 133 publications

(103 reference statements)

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“…It is well known that the isolation of microorganisms is a gold standard for accurate detection of etiological agents of infectious diseases (Paolucci et al, 2010). Furthermore, early detection of bloodstream infections could prevent implantation of microorganisms into vital organs such as the brain, heart or kidneys (Bakowski et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of Antibacterial Susceptibility Patterns of the Bacteria Isolated from Patients' Blood Samples over Two Periods

Japoni¹

2011

American Journal of Infectious Diseases

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Problem statement: Due to continuous changes in the frequencies and antibacterial susceptibility patterns of nosocomial pathogens, periodical surveillance of these fluctuations could help the clinicians to treat hospitalized patients more efficiently whenever empirical therapies need to be considered. This study was conducted to compare the prevalence of the bacteria recovered from bloodstream samples by Bactec 9240, over the two periods of 2001-2004 and 2005-2008 and to evaluate their antibacterial susceptibility patterns. Approach: Totally, 3622 culture positive blood samples were analyzed over the periods. Antibacterial susceptibility patterns of the isolates were determined by standard disk diffusion (Kirby-Bauer) method. The data were compared in terms of quantity and quality of the pathogens and based on their distributions in three main wards. Results: Changes were observed in bacterial composition and frequencies of them, between the two periods. Compared with the first pried, increased frequencies of antibiotic resistant bacteria such as S. aureus, enterococci, acintobacter and pseudomonas were noticed in the second period. Most patients were admitted to pediatrics, followed by adults and neonates wards. Increased antibiotic resistance of the majority of the bacteria in the second period indicates the decreased efficacy of corresponding antibiotics. However, overall efficacy of some antibiotics such as ciprofloxacin and amikacin against Gram positive bacteria preserved. Conclusion: Trend of composition of the bacteria from first to second period could suggest the domination of antibiotic resistant bacteria over the sensitive ones. Appropriate strategies including strict control measures and rational prescription of the effective antibiotics may retard the trend accordingly. Vancomycin and imipenem were the most active antibiotics against Gram positive and negative bacteria. Combination of these two antibiotics is highly recommended for empirical therapy.

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Section: Introductionmentioning

confidence: 99%

Comparative Study of Antibacterial Susceptibility Patterns of the Bacteria Isolated from Patients' Blood Samples over Two Periods

Japoni¹

2011

American Journal of Infectious Diseases

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“…Several nucleic acidbased techniques have been developed for infection diagnosis and pathogen identification (Dark et al, 2009;Paolucci et al, 2010;Ecker et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Correlation between inoculum volume, positivity rates and microorganisms isolated from blood cultures

Azizieh¹,

Dhar²

2014

Afr. J. Microbiol. Res.

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The study aimed to assess the volume of blood inoculum in each culture bottle and its effect on the rate and time to positivity, types of microorganisms isolated and contamination rate. During a period of six months, two sets of blood cultures (aerobic and anaerobic: 3006 bottles each) were collected. Of the bottles collected, only 2916 aerobic and 2898 anaerobic culture bottles were analysed by categorising the samples into three different blood volume groups: < 8 ml, 8-10 ml and >10 ml, where ≥8 ml is considered an adequate volume. In the aerobic bottles, there was no significant difference in the rate of positivity among the three groups: 8-10 ml and < 8 ml (p = 0.226) or > 10 ml (p = 0.282). However, there was a significant decrease in positivity in the anaerobic bottles when comparing the > 10 ml blood group with the < 8 ml group (p = 0.032). The contamination rate was lower in the > 10 ml volume category in aerobic blood cultures (p = 0.008) and the isolation rate of Gram-negative bacilli was higher in this category compared with the other two categories (p = 0.05). Our study shows that the volume of blood cultured was a variable that influences the positivity rate and contamination rate of a blood culture sample.

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“…Molecular techniques have been developed with the aim to improve sensitivity and to detect bloodstream infection earlier [5]. In pneumococcal pneumonia, blood cultures often remain negative and polymerase chain reaction (PCR)-based detection of Streptococcus pneumoniae in patients with a clinical suspicion for pneumonia has been shown to be more sensitive than blood cultures in clinical studies [6,7].…”

Section: Molecular Techniquesmentioning

confidence: 99%