Conventional and Molecular Detection of Pasteurella Multocida in Outbreak of Respiratory Tract Infection of Sheep and Goats in Basrah Province

Abstract

One hundred eight nasal swabs and blood samples from respiratory tract infected animals 66 from sheep and 52 from goats were collected from different sites of Basrah province during a period from December 2012 to April 2013 for isolation and identification of the Pasteurella multocida according to PCR assay . Nasal swabs and blood samples were directly cultured on proper media, then five colonies from the agar plate of suspected P. multocida cultures were used for extracted DNA and, further used for Polymerase chain reaction PCR . PCR was carried out for amplified the PMOut gene on the previously extracted P. multocida DNA . The best amplification of PMOut gene was observed at 45°C annealing temperature . Under these optimal conditions, the expected fragment of 219bp of PMOut gene was successfully amplified. On the other hand , the distinct amplification with a molecular length of 219bp was obtained in 56 positive PCR samples (37 from sheep with distribution rate 56% and 19 from goats with distribution rate 36.5% ). The PCR results of the PMOut gene was found to be potentially a useful method for identification of P. multocida infections.

Isolation and Identification of Pasteurella multocida and Mannheimia haemolytica from Pneumonic Small Ruminants and Their Antibiotic Susceptibility in Haramaya District, Eastern Ethiopia

“…This is in line with the findings of Alemneh and Tewodros [ 4 ]. However, the results of this study were lower than those reported by Rasha et al [ 41 ], who reported pasteurella species from sheep and goats with a recovery rate of 56% and 44%, respectively. Also, this finding was higher than the finding obtained by Alemneh and Tewodros [ 4 ], who reported 37.1% and 21.9% prevalence in sheep and goats, respectively.…”

Pasteurellosis in small ruminants and its epidemiology, prevention and control: A brief review

“…of affected lungs from goat. In Iraq, Pasteurella multocida was found in 4% and Mannheimia haemolytica in 2.6% (Ahmed et al, 2023), 31.4% (Assefa et al, 2018), 21.9% (Alemneh et al, 2016), 36.5% of goats and in 56% of sheep (Rasha et al, 2014). Pneumonic pasteurellosis has been reported in Bangladesh by many investigators where Pasteurella spp.…”