Convenient Assay of O2-Generated on Potato Tuber Tissue Slices Treated with Fungal Elicitor by Electron Spin Resonance - No Secondary Oxidative Burst Induction by H2O2Treatment

Park, Hae Jun; Doke, Noriyuki

doi:10.5423/ppj.2005.21.3.283

Cited by 4 publications

(3 citation statements)

References 11 publications

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“…a, bottom). To confirm that superoxide anions accumulated in bacterial RNA‐infiltrated plants, we quantified the superoxide anions using ESR spectroscopy (Park & Doke, ). The data showed that superoxide anions were clearly produced in response to bacterial RNAs (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Bacterial RNAs activate innate immunity in Arabidopsis

et al. 2015

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Summary The common molecular patterns of microbes play a critical role in the regulation of plant innate immunity. However, little is known about the role of nucleic acids in this process in plants. We pre‐infiltrated Arabidopsis leaves with total RNAs from Pseudomonas syringae pv. tomato DC3000 (Pto DC3000) and subsequently inoculated these plants with the same bacterial cells. Total Pto DC3000 RNAs pre‐infiltrated into Arabidopsis leaves elicited plant immune responses against Pto DC3000. However, sheared RNAs and RNase A application failed to induce immunity, suggesting that intact bacterial RNAs function in plant innate immunity. This notion was supported by the positive regulation of superoxide anion levels, callose deposition, two mitogen‐activated protein kinases and defense‐related genes observed in bacterial RNA‐pre‐treated leaves. Intriguingly, the Pto DC3000 population was not compromised in known pattern recognition receptor mutants for chitin, flagellin and elongation factor‐Tu (EF‐Tu). Plant defense‐related mutant analyses further revealed that bacterial RNA‐elicited innate immunity was normally required for salicylic and jasmonic acid signaling. Notably, among total RNAs, the abundant bacterial RNA species 16S and 23S ribosomal RNAs were the major determinants of this response. Our findings provide evidence that bacterial RNA serves as a microbe‐associated molecular pattern in plants.

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Section: Resultsmentioning

confidence: 99%

“…All experiments were performed as described by Park & Doke (2005) with some modifications. Briefly, Arabidopsis leaf disks (0.6 mm in diameter) were prepared and floated in sterile water for 18 h in the dark for stabilization.…”

Section: Measurement Of Superoxide Anion Levels By Electron Spin Resomentioning

confidence: 99%

Bacterial RNAs activate innate immunity in Arabidopsis

et al. 2015

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“…NADPH oxidase, known as the respiratory burst oxidase, catalyzes the production of O 2 2 by the one-electron reduction of molecular oxygen using NADPH as an electron donor. The rapid transformation of O 2 2 into H 2 O 2 during plant-pathogen interactions was first observed in potato (Solanum tuberosum) tubers infected with an avirulent race of Phytophthora infestans (Doke, 1983;Park and Doke, 2005). Rboh (respiratory burst oxidase homologs) genes encoding gp91 phox (the catalytic subunit of the NADPH oxidase of phagocytes) have been found in rice (Oryza sativa; Groom et al, 1996), Nicotiana benthamiana (Yoshioka et al, 2003), and Arabidopsis (Arabidopsis thaliana; Desikan et al, 1998;Keller et al, 1998;Torres et al, 1998;Laloi et al, 2004;.…”

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confidence: 99%

Hydrogen Peroxide Generation by the Pepper Extracellular Peroxidase CaPO2 Activates Local and Systemic Cell Death and Defense Response to Bacterial Pathogens

et al. 2007

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Reactive oxygen species (ROS) are responsible for mediating cellular defense responses in plants. Controversy has existed over the origin of ROS in plant defense. We have isolated a novel extracellular peroxidase gene, CaPO2, from pepper (Capsicum annuum). Local or systemic expression of CaPO2 is induced in pepper by avirulent Xanthomonas campestris pv vesicatoria (Xcv) infection. We examined the function of the CaPO2 gene in plant defense using the virus-induced gene silencing technique and gain-of-function transgenic plants. CaPO2-silenced pepper plants were highly susceptible to Xcv infection. Virus-induced gene silencing of the CaPO2 gene also compromised hydrogen peroxide (H 2 O 2 ) accumulation and hypersensitive cell death in leaves, both locally and systemically, during avirulent Xcv infection. In contrast, overexpression of CaPO2 in Arabidopsis (Arabidopsis thaliana) conferred enhanced disease resistance accompanied by cell death, H 2 O 2 accumulation, and PR gene induction. In CaPO2-overexpression Arabidopsis leaves infected by Pseudomonas syringae pv tomato, H 2 O 2 generation was sensitive to potassium cyanide (a peroxidase inhibitor) but insensitive to diphenylene iodonium (an NADPH oxidase inhibitor), suggesting that H 2 O 2 generation depends on peroxidase in Arabidopsis. Together, these results indicate that the CaPO2 peroxidase is involved in ROS generation, both locally and systemically, to activate cell death and PR gene induction during the defense response to pathogen invasion.

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Superoxide anion regulates plant growth and tuber development of potato

Kim

et al. 2007

Plant Cell Rep

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A higher concentration of H2O2 was detected in the sense transgenic potato plant (SS4) with the lily chCu,ZnSOD sequence, whereas higher levels of O2(-) was detected in the antisense transgenic plant (SA1) than the WT plant. The elongation growth in SA1 was significantly inhibited by treatment with diphenyleneiodonium, an inhibitor of O2(-) generation, and promoted in the SS4 on treatment with herbicide methyl viologen, a generator of apoplastic O2(-) . Higher concentrations of GAs were detected during plant growth and the early stage of tuberization in SA1. Complete recovery of the above elongation growth and microtuberization pattern in transgenic plants following treatment of GA(3) or an inhibitor of gibberellin synthesis, paclobutrazol, indicate that these changes were mainly caused by active GA levels. In conclusion, a specific ROS (O2(-) ) acts as a signal transducer via GA biosynthetic pathways for the regulation of plant growth and tuber development of potato.

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Convenient Assay of O₂^-Generated on Potato Tuber Tissue Slices Treated with Fungal Elicitor by Electron Spin Resonance - No Secondary Oxidative Burst Induction by H₂O₂Treatment

Cited by 4 publications

References 11 publications

Bacterial RNAs activate innate immunity in Arabidopsis

Bacterial RNAs activate innate immunity in Arabidopsis

Hydrogen Peroxide Generation by the Pepper Extracellular Peroxidase CaPO2 Activates Local and Systemic Cell Death and Defense Response to Bacterial Pathogens

Superoxide anion regulates plant growth and tuber development of potato

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