Background: Launaea nudicaulis (L.) Hook. f. is a glabrous perennial herb having simple, sessile leaf and yellow flower. In Ayurveda it is used as a botanical source of Gojihva. The herb is said to be useful as a Shaka (vegetable) and medicine for various diseases like Raktapitta (hemorrhagic disorders), Shwaasa (breathing problems), Kasa (cough), Kushtha (skin diseases), Vrana (wounds), Visarpa (erysipelas), Prameha (diabetes mellitus), and Aruchi (anorexia). Few researches have also been published on its analgesic, anti-inflammatory activity, anti-diabetic and wound healing but its micromorphology, pharmacognosy, physiochemical and bio-element analysis is not reported yet. Objectives: The present study was carried out to study the micromorphology, pharmacognosy, physiochemical and bio-element analysis for proper identification and authentication of the herb. Materials and Methods: The macroscopy, microscopy, physicochemical, phytochemical parameters and bio-element analysis were done using different chemicals and reagents with standard procedures. Results: The presence of golden yellow latex in the root of the herb is identification characteristic. Calcium oxalate crystals and stone cells were seen in powder study. The GC-MS analysis identified 25 metabolites which comprised of fatty acids, sugars, amino acids etc. HPLC analysis revealed presence of 10 metabolites among which Cichoric acid was found in maximum concentration. Conclusion: The results of the study will help for the proper identification and authentication of the herb.