Controlling the orientation of immobilized proteins on an affinity membrane through chelation of a histidine tag to a chitosan-Ni++ surface

Exploiting the interactions between poly-histidine fusion tags and immobilized metal ions

“…Alternatively, for analytical applications, Ahmed et al (2006) poly-histidine tagged fusion proteins (Noedl et al, 2005). Consequently, by adding an epitope tag onto any target protein, a universal method for following the given protein can be established by using a generic assay for detecting the known molecular handle that has been added.…”

Chitosan Based Membranes for Separation, Pervaporation and Fuel Cell Applications: Recent Developments

“…The superiority of sorption capability of CS-HT membrane is also attributable to its larger specific surface area than that of CS-LT membrane. The results obtained were conducive to the design and fabrication of chiral ligand exchange membranes for enantiomeric separation in sorption mode [63]. Affinity membranes based on a Ni 2+ −chelating CS surface were fabricated to immobilize C-terminus hexa histidine-tagged green fluorescent protein (His-GFP) [64].…”

Binding Ni(II) ions to chitosan and its N-heterocyclic derivatives: Density functional theory investigation

“…A majority of studies on complexation mechanism were focused on coordination of Cu(II), but some experimental and theoretical studies were performed for other metal ions [10,12,17,20]. Coordination of Ni 2+ ions by chitosan have not yet been extensively investigated, although functional chitosan-based materials containing nickel are currently of high interest in numerous applied fields, e.g., catalysis [23], protein separation [24], and selective sorption [25].…”

“…The geometry of Ni 2+ -polymer complexes is of great importance, if orientation of macromolecules on the surface determines their biological activity [24]. Thus, theoretical investigations of Ni 2+ ions binding to chitosan and its derivatives with enhanced chelating properties can provide valuable information on the complexes structure and stability, which are required for development of chitosan-based functional materials with specific properties.…”