Controlling the Magnetic Field Distribution on the Micrometer Scale and Generation of Magnetic Bead Patterns for Microfluidic Applications

Yu, Xu; Feng, Xuan; Hu, Jun; Zhang, Zhiling; Pang, Dai‐Wen

doi:10.1021/la104400m

Cited by 40 publications

(52 citation statements)

References 47 publications

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“…The SPMBs with carboxyl groups were functionalized with C DNA according to our previous reports (He et al 2014; Yu et al 2011; Yu et al 2014; Yu et al 2013). Briefly, 40 μL of 50.0 mg/mL SPMBs were first washed three times with MES buffer (50.0 mM MES, pH = 6.0).…”

Section: Methodsmentioning

confidence: 99%

“…Magnetic beads have been widely used in biological sample preparation and detection assays due to their benefits of large surface-to-volume ratio, easy manipulation, flexible functionalization, and excellent biocompatibility (Chen et al 2010; Yu et al 2011; Yu et al 2014; Yu et al 2013). Owing to the ease of separation and enrichment of the targets, magnetic beads were widely used to improve the selectivity and sensitivity of various analytical methods.…”

Section: Introductionmentioning

confidence: 99%

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Highly sensitive DNA detection using cascade amplification strategy based on hybridization chain reaction and enzyme-induced metallization

Zhang

Zheng

2015

Biosensors and Bioelectronics

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A novel highly sensitive colorimetric assay for DNA detection using cascade amplification strategy based on hybridization chain reaction and enzyme-induced metallization was established. The DNA modified superparamagnetic beads were demonstrated to capture and enrich the target DNA in the hybridization buffer or human plasma. The hybridization chain reaction and enzyme-induced silver metallization on the gold nanoparticles were used as cascade signal amplification for the detection of target DNA. The metalization of silver on the gold nanoparticles induced a significant colour change from red to yellow until black depending on the concentration of the target DNA, which could be recognized by naked eyes. This method showed a good specificity for the target DNA detection, with the capabilty to discriminate single-base-pair mismatched DNA mutation (single nucleotide polymorphism). Meanwhile, this approach exhibited an excellent anti-interference capability with the convenience of the magentic seperation and washing, which enabled its usage in complex biological systems such as human blood plasma. As an added benefit, the utilization of hybridization chain reaction and enzyme-induced metallization improved detection sensitivity down to 10 pM, which is about 100-fold lower than that of traditional unamplified homogeneous assays.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Highly sensitive DNA detection using cascade amplification strategy based on hybridization chain reaction and enzyme-induced metallization

Zhang

Zheng

2015

Biosensors and Bioelectronics

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“…To improve the interaction on the ZnO nanorod surface, herringbone structures were incorporated inside the microfluidic device for enhanced mixing . Highly selective detection of H5N2 subtype AIV is achieved by fast continuous flow in the microfluidic channels that effectively minimizes nonspecific adsorption . The entire detection process could be completed within 1.5 h, while the limit of detection (LOD) for the H5N2 AIV was measured as 3.6 × 10 3 EID 50 mL −1 .…”

Section: Introductionmentioning

confidence: 99%

A Nanostructured Microfluidic Immunoassay Platform for Highly Sensitive Infectious Pathogen Detection

Xia

Tang

et al. 2017

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Rapid and simultaneous detection of multiple potential pathogens by portable devices can facilitate early diagnosis of infectious diseases, and allow for rapid and effective implementation of disease prevention and treatment measures. The development of a ZnO nanorod integrated microdevice as a multiplex immunofluorescence platform for highly sensitive and selective detection of avian influenza virus (AIV) is described. The 3D morphology and unique optical property of the ZnO nanorods boost the detection limit of the H5N2 AIV to as low as 3.6 × 10 EID mL (EID : 50% embryo infectious dose), which is ≈22 times more sensitive than conventional enzyme-linked immunosorbent assay. The entire virus capture and detection process could be completed within 1.5 h with excellent selectivity. Moreover, this microfluidic biosensor is capable of detecting multiple viruses simultaneously by spatial encoding of capture antibodies. One prominent feature of the device is that the captured H5N2 AIV can be released by simply dissolving ZnO nanorods under slightly acidic environment for subsequent off-chip analyses. As a whole, this platform provides a powerful tool for rapid detection of multiple pathogens, which may extent to the other fields for low-cost and convenient biomarker detection.

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“…In general, magnetic particles as induced by a magnetic field can self-assemble, resulting in a 1D, 2D or 3D structure and morphology [1][2][3][4]. It has been reported that self-assembled materials with 3D structures demonstrate more remarkable application prospects in magnetic recording devices, optoelectronic devices, biological sensors, etc.…”

Section: Introductionmentioning

confidence: 99%

Influences of different parameters on the microstructure of magnetic-field-induced self-assembled film

Yang

2016

IOP Conf. Ser.: Mater. Sci. Eng.

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Theoretical study of the morphology of self-assembled amphoteric oxide colloid nanocrystals in weak electrolyte solutions A V Alfimov, E M Aryslanova and S A Chivilikhin -Magnetic-field-induced 1st order transition to FFLO state at paramagnetic limit in 2D superconductors N A Fortune, C C Agosta, S T Hannahs et al. Nanjing Xiaozhuang University, School of Environmental Science, Nanjing, P.R. China E-mail: xiaozhuangxxc2010@126.com Abstract. Self-assembled films with needle-like microarrays were fabricated using a mixture of cobalt and fluorocarbon resin under a magnetic field. The various influences of magnetic powder content, viscosity and size distribution on the structure of the self-assembled films were investigated. The self-assembled film morphologies were characterized by stereomicroscope and scanning electron microscopy. Experimental results indicate that an increase in magnetic powder content results in greater unit height and diameter, and that a reduction in viscosity results in increasing array density and decreasing unit width. Additionally, particles with narrow size distribution were able to attain more regular microarray structures. The structural alterations were closely related to numerous effects such as van der Waals forces, dipole-dipole interactions, and external-dipole interactions. The self-assembled film demonstrated magnetic anisotropy, as identified by vibrating sample magnetometry (VSM).

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Controlling the Magnetic Field Distribution on the Micrometer Scale and Generation of Magnetic Bead Patterns for Microfluidic Applications

Cited by 40 publications

References 47 publications

Highly sensitive DNA detection using cascade amplification strategy based on hybridization chain reaction and enzyme-induced metallization

Highly sensitive DNA detection using cascade amplification strategy based on hybridization chain reaction and enzyme-induced metallization

A Nanostructured Microfluidic Immunoassay Platform for Highly Sensitive Infectious Pathogen Detection

Influences of different parameters on the microstructure of magnetic-field-induced self-assembled film

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