Controlling the Cell Adhesion Property of Silk Films by Graft Polymerization

Dhyani, Vartika; Singh, Neetu

doi:10.1021/am4060595

Cited by 30 publications

(47 citation statements)

References 29 publications

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“…It is necessary that, among these newly introduced functional groups, the amino and carboxyl groups are represented, as they are used to bind proteins to the surface of the polymer. Grafting the surface of the polymer with growth factors also facilitates cell adhesion and proliferation [11]. …”

Section: Introductionmentioning

confidence: 99%

Surface Modification of Polymer Substrates for Biomedical Applications

2017

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While polymers are widely utilized materials in the biomedical industry, they are rarely used in an unmodified state. Some kind of a surface treatment is often necessary to achieve properties suitable for specific applications. There are multiple methods of surface treatment, each with their own pros and cons, such as plasma and laser treatment, UV lamp modification, etching, grafting, metallization, ion sputtering and others. An appropriate treatment can change the physico-chemical properties of the surface of a polymer in a way that makes it attractive for a variety of biological compounds, or, on the contrary, makes the polymer exhibit antibacterial or cytotoxic properties, thus making the polymer usable in a variety of biomedical applications. This review examines four popular methods of polymer surface modification: laser treatment, ion implantation, plasma treatment and nanoparticle grafting. Surface treatment-induced changes of the physico-chemical properties, morphology, chemical composition and biocompatibility of a variety of polymer substrates are studied. Relevant biological methods are used to determine the influence of various surface treatments and grafting processes on the biocompatibility of the new surfaces—mammalian cell adhesion and proliferation is studied as well as other potential applications of the surface-treated polymer substrates in the biomedical industry.

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Section: Introductionmentioning

confidence: 99%

Surface Modification of Polymer Substrates for Biomedical Applications

2017

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“…Our approach gives a unique opportunity to tune thickness, morphology, structure, and biodegradability rate of silk films and capsules by varying silk secondary structure and PVCL length. 63,64 To investigate degradation behavior of silk hydrogen-bonded with PVCL in the presence of enzyme, SA and dipped (silk/PVCL) films of 40 nm were constructed at pH=7.5 and incubated at 37°C in presence of Protease XIV from Streptomyces griseus for 24 hours. 1,2,3 Silk has a unique block-copolymer structure where hydrophobic β-sheet blocks of (Ala-Gly) n -repeats are linked by hydrophilic less ordered regions containing Tyr, Val, and ionized acidic and basic amino acids.…”

mentioning

confidence: 99%

“…Film thickness, surface morphology, wettability, and pH-stability are strongly controlled by deposition conditions and PVCL molecular weight. 63,64 To investigate degradation behavior of silk hydrogen-bonded with PVCL in the presence of enzyme, SA and dipped (silk/PVCL) films of 40 nm were constructed at pH=7.5 and incubated at 37°C in presence of Protease XIV from Streptomyces griseus for 24 hours. On the other hand, successful SA assemblies at neutral pH point to strong hydrophobic interactions between PVCL and crystalline silk domains that facilitate multilayer growth despite the excess charge from ionized silk acidic and basic groups.…”

mentioning

confidence: 99%

Tuning assembly and enzymatic degradation of silk/poly(N-vinylcaprolactam) multilayers via molecular weight and hydrophobicity

et al. 2015

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We report on enzymatically degradable nanothin coatings obtained by layer-by-layer (LbL) assembly of silk fibroin with poly(N-vinylcaprolactam) (PVCL) via hydrogen bonding and hydrophobic interactions. We found that both silk β-sheet content, controlled through dipping and spin-assisted LbL, and PVCL molecular weight regulate film thickness, microstructure, pH-stability, and biodegradability with a nanoscale precision. Thickness of (silk/PVCL) films increased with increase in PVCL molecular weight and decrease in deposition pH. The impact of assembly pH on film growth was more dramatic for dipped films. These systems show a significant rise in thickness with increase in PVCL molecular weight at pH < 5 but become independent on polymer chain length at pH ≥ 5. We also found that spin-assisted films exhibited a greater stability at elevated pH and against enzymatic degradation as compared to their dipped counterparts. For both film types, the pH and enzymatic stability was improved with increasing PVCL length and β-sheet content, indicating enhanced hydrophobic and hydrogen-bonded interactions between PVCL and silk. Finally, we fabricated spherical and cubical (silk/PVCL) LbL capsules of regulated permeability and enzymatic degradation. Our approach gives a unique opportunity to tune thickness, morphology, structure, and biodegradability rate of silk films and capsules by varying silk secondary structure and PVCL length. Accounting for all-aqueous fabrication and the biocompatibility of both polymers these biodegradable materials provide novel platforms for delivery systems and medical devices.

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“…Without functionalization Physical crosslinking [57] Blended with stabilizing component (e.g., gelatin [57] ) Enzymatic crosslinking by tyrosinase [39] Biodegradable Poor cell adhesion due to hydrophobic character [58] Common sources: B. mori silkworm Recombinant silk protein eADF4(C16) mimicking Araneus diadematus silk protein sequences [59] Poly-saccharides Agarose d-Galactose and 3,6-anhydro-l-galactopyranose Thermoreversible gelation…”

Section: Biodegradablementioning

confidence: 99%

From Shape to Function: The Next Step in Bioprinting

et al. 2020

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In 2013, the “biofabrication window” was introduced to reflect the processing challenge for the fields of biofabrication and bioprinting. At that time, the lack of printable materials that could serve as cell‐laden bioinks, as well as the limitations of printing and assembly methods, presented a major constraint. However, recent developments have now resulted in the availability of a plethora of bioinks, new printing approaches, and the technological advancement of established techniques. Nevertheless, it remains largely unknown which materials and technical parameters are essential for the fabrication of intrinsically hierarchical cell–material constructs that truly mimic biologically functional tissue. In order to achieve this, it is urged that the field now shift its focus from materials and technologies toward the biological development of the resulting constructs. Therefore, herein, the recent material and technological advances since the introduction of the biofabrication window are briefly summarized, i.e., approaches how to generate shape, to then focus the discussion on how to acquire the biological function within this context. In particular, a vision of how biological function can evolve from the possibility to determine shape is outlined.

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Controlling the Cell Adhesion Property of Silk Films by Graft Polymerization

Cited by 30 publications

References 29 publications

Surface Modification of Polymer Substrates for Biomedical Applications

Surface Modification of Polymer Substrates for Biomedical Applications

Tuning assembly and enzymatic degradation of silk/poly(N-vinylcaprolactam) multilayers via molecular weight and hydrophobicity

From Shape to Function: The Next Step in Bioprinting

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