Controlling the Ambient Air Affected Reactive Species Composition in the Effluent of an Argon Plasma Jet

Reuter, Stephan; Winter, J.; Schmidt-Bleker, Ansgar; Tresp, Helena; Hammer, Malte U.; Weltmann, Klaus‐Dieter

doi:10.1109/tps.2012.2204280

Cited by 123 publications

(118 citation statements)

References 17 publications

Supporting

Mentioning

116

Contrasting

Order By: Relevance

“…The CAP source studied here is the kinpen Sci, introduced in ref . The kinpen Sci is used with a shielding gas device which produces a gas curtain of defined composition around the active plasma plume as illustrated in Figure and previously presented in ref . To investigate the composition and concentration of the produced RONS in the far‐field of the jet, a Fourier transform infrared (FTIR) spectrometry setup described in ref .…”

Section: Methodsmentioning

confidence: 99%

How to produce an NO_x- instead of O_x-based chemistry with a cold atmospheric plasma jet

Schmidt-Bleker

Bansemer

Reuter

et al. 2016

Plasma Process. Polym.

Self Cite

View full text Add to dashboard Cite

A novel method is presented, which allows to generate a nitrogen oxides (NOx)‐dominated chemistry with a cold atmospheric plasma (CAP) jet which typically produces an oxygen‐based reactive species (Ox) cocktail. The reactive oxygen and nitrogen (RONS) production of the CAP jet kinpen is monitored using Fourier transform infrared (FTIR) absorption spectroscopy. While in previous approaches, the plasma chemistry of CAP jets operated with noble gases has been influenced with molecular feed gas admixtures of O2 and N2, humidified feed gas, and shielding gas, these methods are now combined in order to obtain a previously unattainable range of operating regimes: The plasma chemistry can be tuned continuously from Ox to NOx dominated, while the degree of oxidation of the produced NOx can easily be influenced.

show abstract

Section: Methodsmentioning

confidence: 99%

How to produce an NO_x- instead of O_x-based chemistry with a cold atmospheric plasma jet

Schmidt-Bleker

Bansemer

Reuter

et al. 2016

Plasma Process. Polym.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Convective and diffusive mass transport of argon, air, and shielding gas is implemented as described in [18]. A working gas inflow of pure argon and an inflow rate of pure shielding gas are fixed at 5 slm each.…”

Section: A Fluid Dynamics Simulationmentioning

confidence: 99%

“…Plasma treatment of liquids with a designed effluent reactive species composition can tailor the reactive nitrogen and oxygen species concentrations within the treated liquid [18]. In this paper, plasma-induced nitrite concentration in our standard cell culture medium (described in Section II) is investigated.…”

Section: Nitrite and Nitrate Assaymentioning

confidence: 99%

See 1 more Smart Citation

From RONS to ROS: Tailoring Plasma Jet Treatment of Skin Cells

Reuter

Tresp

Wende

et al. 2012

IEEE Trans. Plasma Sci.

Self Cite

171

142

View full text Add to dashboard Cite

Finding a solution for air species contamination of atmospheric pressure plasmas in plasma medical treatment is a major task for the new field of plasma medicine. Several approaches use complex climate chambers to control the surrounding atmosphere. In this paper, ambient species are excluded in plasma-human-skin-cell treatment by ensheathing the plasma jet effluent with a shielding gas. Not only does this gas curtain protect the plasma jet effluent from inflow of air species but it also, more importantly, allows controlling the effluent reactive species composition by adjusting the mixture of the shielding gas. In the present investigations, the mixture of nitrogen to oxygen within the gas curtain around an argon atmospheric pressure plasma jet (kinpen) is varied. The resulting reactive plasma components produced in the jet effluent are thus either oxygen or nitrogen dominated. With this gas curtain, the effect of reactive oxygen species (ROS) and reactive nitrogen species (RNS) on the cell viability of indirectly plasma-treated HaCaT skin cells is studied. This human keratinocyte cell line is an established standard for a skin model system. The cell viability is determined by a fluorometric assay, where metabolically active cells transform nonfluorescent resazurin to the highly fluorescent resorufin. Plasma jet and gas curtain are characterized by numerical flow simulation as well as by optical emission spectroscopy. The generation of nitrite within the used standard cell culture medium serves as a measure for generated RNS. Measurements with the leukodye dichlorodihydrofluorescein diacetate show that, despite a variation of the shielding gas mixture, the total amount of generated reactive oxygen plus nitrogen species is constant. It is shown that a plasma dominated by RNS disrupts cellular growth less than a ROSdominated plasma.Index Terms-Atmospheric pressure plasma jet, gas curtain, plasma liquid interaction, plasma medicine, reactive nitrogen species (RNS), reactive oxygen plus nitrogen species (RONS), reactive oxygen species (ROS), skin cells.

show abstract

“…We utilized the well-characterized [23] atmospheric pressure argon plasma jet kinpen09 as plasma source to manipulate excess growth and metastatic characteristic of malignant cells. This plasma jet has proven to be safe during its use [24] and generates a wealth of reactive species [25] which can be delivered to cells [26] and tissues [27] alike. The failure of a functional antioxidant defense system in cancer cells as well as the different sensitization of malignant cells trough plasma illustrates a starting point for a plasma-based strategy in adjuvant cancer therapy [28].…”

Section: Introductionmentioning

confidence: 99%

Cell migration and adhesion of a human melanoma cell line is decreased by cold plasma treatment

Schmidt

Bekeschus

Woedtke

et al. 2015

Clinical Plasma Medicine

View full text Add to dashboard Cite

Controlling the Ambient Air Affected Reactive Species Composition in the Effluent of an Argon Plasma Jet

Cited by 123 publications

References 17 publications

How to produce an NO_x- instead of O_x-based chemistry with a cold atmospheric plasma jet

How to produce an NO_x- instead of O_x-based chemistry with a cold atmospheric plasma jet

From RONS to ROS: Tailoring Plasma Jet Treatment of Skin Cells

Cell migration and adhesion of a human melanoma cell line is decreased by cold plasma treatment

Contact Info

Product

Resources

About

Controlling the Ambient Air Affected Reactive Species Composition in the Effluent of an Argon Plasma Jet

Cited by 123 publications

References 17 publications

How to produce an NOx- instead of Ox-based chemistry with a cold atmospheric plasma jet

How to produce an NOx- instead of Ox-based chemistry with a cold atmospheric plasma jet

From RONS to ROS: Tailoring Plasma Jet Treatment of Skin Cells

Cell migration and adhesion of a human melanoma cell line is decreased by cold plasma treatment

Contact Info

Product

Resources

About

How to produce an NO_x- instead of O_x-based chemistry with a cold atmospheric plasma jet

How to produce an NO_x- instead of O_x-based chemistry with a cold atmospheric plasma jet