Controlling sustained statins release in multi-layered composite scaffolds for healing of osteoporotic bone defects

Li, Xilin; Li, Ting; Wang, Fei; Sun, Fanxi; Hu, Jiang; Ye, Xiaojian; Wang, Dongsheng; Yang, Xiao

doi:10.1016/j.bioadv.2022.212838

Cited by 8 publications

(6 citation statements)

References 34 publications

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“…This was consistent with the above results for the injection force and the previous literature that a higher M w , concentration and solid content in solution facilitated a sufficient mechanical strength of the porous scaffolds that would be suitable for tissue regeneration. 39,57 It was also observed from Fig. 3 that the microstructure of the supportive matrix was loose for the materials with poor mechanical strength (Fig.…”

Section: Compression Property and Microstructurementioning

confidence: 77%

Section: Resultsmentioning

confidence: 99%

“…[35][36][37][38] Therefore, in this study, GMs were fabricated and proposed not only as a porogen to effectively balance the contradiction between the scaffold porosity and mechanical strength, but also as a delivery vehicle for bone morphogenetic protein 2 (BMP-2) to overcome the short half-life of the growth factors and improve the osteogenesis of the injectable composite scaffolds. 39 2. Experimental section 2.1.…”

Section: Introductionmentioning

confidence: 99%

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In situ-formed hydroxyapatite and poly (lactic-co-glycolic acid) injectable implants as the cargo loading of bioactive substances for bone regeneration

et al. 2023

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Section: Compression Property and Microstructurementioning

confidence: 77%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In situ-formed hydroxyapatite and poly (lactic-co-glycolic acid) injectable implants as the cargo loading of bioactive substances for bone regeneration

et al. 2023

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“…Cells were added in 96‐well plates (1 × 10 4 /well) overnight, and then incubated with indicated concentrations of hederagenin under NG or HG conditions for 48 h, followed by incubation with 10 μl of CCK‐8 solution (Beyotime) for 2 h. The absorbance at 450 nm was determined using a microplate reader (Biotek). Cell proliferation was assessed using the following equation: cell proliferation (%) = (absorbance of experiment group − absorbance of blank group)/(absorbance of control group − absorbance of blank group) × 100% (Liu et al, 2022).…”

Section: Methodsmentioning

confidence: 99%

Hederagenin inhibits high glucose‐induced fibrosis in human renal cells by suppression of NLRP3 inflammasome activation through reducing cathepsin B expression

Yang,

Jiang

et al. 2023

Chem Biol Drug Des

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Diabetic nephropathy is a major complication of diabetes mellitus and is related to dysfunction of renal cells. Hederagenin is a triterpenoid saponin from some Chinese herbs with anti‐inflammatory and anti‐diabetic activities. However, its role in diabetic nephropathy progression is still obscure. This study aimed to explore the effects of hederagenin on renal cell dysfunction in vitro. Human renal mesangial cells (HRMCs) and human renal proximal tubular epithelial cells (HRPTEpiCs) were cultured under high glucose (HG) conditions to mimic diabetic nephropathy‐like injury. Cell proliferation was evaluated by CCK‐8. mRNA and protein levels were determined by qRT‐PCR and western blotting, respectively. The secretion levels of fibrosis‐related biomarkers were analyzed by ELISA. Results showed that hederagenin reduced HG‐induced proliferation increase in HRMCs and HRPTEpiCs. Hederagenin attenuated HG‐induced increase in mRNA and protein expression of NLRP3, ASC, and IL‐1β. Hederagenin also suppressed HG‐induced increase in mRNA and secretion levels of FN, Col. IV, PAI‐1, and TGF‐β1. NLRP3 inhibitor MCC950 attenuated HG‐induced fibrosis of renal cells, and its activator nigericin reversed the suppressive effect of hederagenin on HG‐induced fibrosis. Bioinformatics analysis predicted cathepsin B (CTSB) as a target of hederagenin to modulate NOD‐like receptor (NLR) pathway. Hederagenin decreased CTSB level, and CTSB overexpression reversed the suppressive effect of hederagenin on HG‐induced NLRP3 inflammasome activation and fibrosis in HRMCs and HRPTEpiCs. In conclusion, hederagenin attenuates HG‐induced fibrosis of renal cells by inhibiting NLRP3 inflammasome activation via reducing CTSB expression, indicating a therapeutic potential of hederagenin in diabetic nephropathy.

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“…On the one hand, they are useful for studying and improving the integration of the newly formed tissue with the surrounding environment [95]; on the other hand, they are considered valid 3D models for bone disease studies [96]. Indeed, 3D composite bone tissue scaffolds with both mechanical stability and drug-delivery functionality are employed to study the drug-delivery properties of statins, biocompatibility, alkaline phosphatase activity, and osteoblasts activity in vitro for the treatment of osteoporosis [97]. Iordachescu and colleagues have realized a micron-scale bone organoid prototype (defined as a human trabecular organoid) to study the effects of microgravity, degenerative events, and related temporal events in bone remodeling, which cannot be reproduced using other technologies in vitro and in vivo [98].…”

Section: Applicationsmentioning

confidence: 99%

Three-Dimensional Cell Cultures: The Bridge between In Vitro and In Vivo Models

Urzì,

Gasparro,

Costanzo

et al. 2023

IJMS

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Although historically, the traditional bidimensional in vitro cell system has been widely used in research, providing much fundamental information regarding cellular functions and signaling pathways as well as nuclear activities, the simplicity of this system does not fully reflect the heterogeneity and complexity of the in vivo systems. From this arises the need to use animals for experimental research and in vivo testing. Nevertheless, animal use in experimentation presents various aspects of complexity, such as ethical issues, which led Russell and Burch in 1959 to formulate the 3R (Replacement, Reduction, and Refinement) principle, underlying the urgent need to introduce non-animal-based methods in research. Considering this, three-dimensional (3D) models emerged in the scientific community as a bridge between in vitro and in vivo models, allowing for the achievement of cell differentiation and complexity while avoiding the use of animals in experimental research. The purpose of this review is to provide a general overview of the most common methods to establish 3D cell culture and to discuss their promising applications. Three-dimensional cell cultures have been employed as models to study both organ physiology and diseases; moreover, they represent a valuable tool for studying many aspects of cancer. Finally, the possibility of using 3D models for drug screening and regenerative medicine paves the way for the development of new therapeutic opportunities for many diseases.

show abstract

Controlling sustained statins release in multi-layered composite scaffolds for healing of osteoporotic bone defects

Cited by 8 publications

References 34 publications

In situ-formed hydroxyapatite and poly (lactic-co-glycolic acid) injectable implants as the cargo loading of bioactive substances for bone regeneration

In situ-formed hydroxyapatite and poly (lactic-co-glycolic acid) injectable implants as the cargo loading of bioactive substances for bone regeneration

Hederagenin inhibits high glucose‐induced fibrosis in human renal cells by suppression of NLRP3 inflammasome activation through reducing cathepsin B expression

Three-Dimensional Cell Cultures: The Bridge between In Vitro and In Vivo Models

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