Controlling simultaneous production of endoglucanase and beta-glucosidase by Fusarium oxysporum in submerged culture

Abstract

The simultaneous production of endoglucanase and fl-glucosidase by Fusarium oxysporum was investigated in submerged culture. Consecutive optimization of growth conditions resulted in the correction of large activity differences, observed during production of enzymes, and substantially enhanced low enzyme yields. At optimum growth conditions yields as high as 1650 and 232 U per g of carbon source of endoglucanase and fi-glucosidase were obtained respectively competing favourably with those reported for microorganisms grown on the same car&m source. The most important kinetic characteristics of the enzymes were the high temperature optima of endoglucanase (6~C) and fl-glucosidase (65°C) and the exceptionally high thermostability of endoglucanase, The latter enzyme retained 50Ue of the activity at pH 5.0 after approximately 6.5 h at 7~C

Cloning and expression of β-glucosidase gene from Bacillus licheniformis into E. coli BL 21 (DE3)

“…• C. This is in agreement with the findings of Riou et al (1998) and Dhake & Patil (2005), while Choi et al (2009) ther increase in temperature resulted in the decrease in activity and coagulation of enzyme occurring at 60 However, Wright et al (1992) and Christakopoulos et al (1995) obtainted maximum β-glucosidase activity at 60…”

Production of cellulolytic enzymes from ascomycetes: Comparison of solid state and submerged fermentation

“…However, when the same strain was grown under SSF conditions and with the same substrate, the CMCase activity was 3 times higher resulting in 1044 U/ml [124]. For F. oxysporum (F3) wheat straw yielded a higher CMCase activity compared to the same strain grown under similar conditions using WB, however, it is the opposite for BG activity [150]. This indicates that the selection of substrate is important in order to obtain highest enzyme activity, e.g.…”

Metabolic engineering and thermodynamic characterization of an extracellular -glucosidase produced by Aspergillus niger

“…respectively. However, Christakopoulos et al (1995) and Jeya et al (2010) obtained maximum β-glucosidase activity at 65°C from Fusarium oxysporum and Penicillium Purpurogenum respectively. Thermodynamic studies of β-glucosidase were carried out using Arrhenius plot and activation energy (E a ), change in enthalpy (∆H) and change in entropy (∆S) were calculated to be 52.17 KJ/mol, 49.90 KJ/mol and -71.69 J/mol.K respectively (Figures 8 and 9).…”

“…It is generally recognized as safe by the Food and Drug Administration (Tailor and Richardson, 1979). β-Glucosidase can be produced both by solid-state and submerged fermentation techniques (Christakopoulos et al, 1995;Iembo et al, 2002;Fawzi, 2003;Garcia-Kirchner et al, 2005;Daroit et al, 2007;Leite et al, 2007). Sub-merged fermentation offers several advantages, among them a possibility to automate many operations, the ease with which various parameters can be monitored (by periodic sampling of broth) and controlled if necessary by the addition of further nutrients or reagents and easier removal of heat generated during the microbial growth in a large scale reactor (Jakubikova et al, 2006).…”