Controlling production of brominated cyclic depsipeptides by Pseudoalteromonas maricaloris KMM 636T

Abstract

Aims: This study aims at evaluating the impact of the nutrient medium components on the in vitro production of the cytotoxic alterochromides. Methods and Results: The employment matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) facilitated the identification of a range of brominated cyclic depsipeptides with molecular masses of 843/845, 857/859 and 922/924/926 Da, and 936/938/940 Da produced by the marine bacterium Pseudoalteromonas maricaloris KMM 636T . The fractions of cytotoxic yellow pigments yielded after methanol extraction of P. maricaloris KMM 636T cells grown on five nutrient media were solely composed of brominated cyclic depsipeptides. Bromo-alterochromides A and B were obtained after cultivation on low nutrient media, while dibrominated derivatives were the principal components of the biosynthesis during cultivation on nutrient rich media. Conclusions: The quantity of bromo-alterochromides and their dibromo-derivates varied depending on the media composition. Significance and Impact of the Study: MALDI-TOF mass spectrometry enables to generate accurate mass analysis for the identification of peptide and its derivates which is important in controlling the production of biologically active compounds in vitro.

A potential bacterial biocontrol agent, strain S2V2 against pathogenic marine Vibrio in aquaculture

“…P. maricolaris produces depsipeptide antibiotics, bromoalterochromides A and B. These substances are non extracellular products, but cell-bound substance (Ivanova et al 2002b;Sobolevskaya et al 2005). P. issachenkonii, P. piscicida and P. ruthenica produce antimicrobial substance in culture supernatant, but P. peptydolytica produces such substance as cell-bound product (Kalinovskaya et al 2004).…”

Characterization of Antarctic psychrotrophic bacteria with antibacterial activities against terrestrial microorganisms

“…Sobolevskaya et al [12] and Zheng et al [9] reported about the antimicrobial compounds extracted from Pseudoalteromonas strains isolated from marine sponges, while Isnansetyo and Kamei [27] deeply characterized the newly discovered P. phenolica, a bacterium producing anti-methicillin-resistant S. aureus substances. Finally, antimicrobial activity has been often associated with Pseudomonas species [16].…”

“…In particular, special attention has been directed to microorganisms isolated from invertebrates, such as sponges and algae [3 -9]. In some cases, bactericidal substances with novel chemical structures were purified and characterized [1,[10][11][12]. Nevertheless, in recent decades there has been a dearth of new classes of discovered antibiotics [5,13].…”

Bioactive Compound Synthetic Capacity and Ecological Significance of Marine Bacterial Genus Pseudoalteromonas

“…Strains were also described as being able to inhibit the growth of yeast [9]. P. maricaloris, a close relative of P. piscicida isolated from the Coral Sea sponge Fascaplysinopsis reticulata, forms yellow colored dibromo-and bromoalterochomides ( Figure 5F) that possess antimicrobial activity and as well as cytotoxicity against the larvae of a sea urchin [95]. The algicidal strain Y, also closely related to P. piscicida, forms broad spectrum antimicrobial yellow brominated substances that LC-MS analysis revealed to comprise 12 different but likely structurally similar compounds.…”