Controlling Metal‐Enhanced Fluorescence Using Bimetallic Nanoparticles

Dutta, Debosruti; Chowdhury, Sanchari; Yang, Chi; Bhethanabotla, Venkat R.; Joseph, Babu

doi:10.1002/9781119325161.ch4

Cited by 2 publications

(1 citation statement)

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“…Fluorescent dyes were assumed to be randomly distributed in terms of both orientation and position. Also it was assumed that the center of mitochondrion coincides with the dipole position by way of ensemble-average effects. , The 3 × 3 PNAs were assumed with the dipole located above the center nanohole. The location in the z -axis was varied nonuniformly to increase memory efficiency with the minimum 10 nm above the metal surface, which considers cell focal contact and membrane thickness.…”

Section: Methodsmentioning

confidence: 99%

Superlocalized Three-Dimensional Live Imaging of Mitochondrial Dynamics in Neurons Using Plasmonic Nanohole Arrays

Son¹,

Lee

Lee³

et al. 2019

ACS Nano

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We investigated the transport of neuronal mitochondria using superlocalized near-fields with plasmonic nanohole arrays (PNAs). Compared to traditional imaging techniques, PNAs create a massive array of superlocalized light beams and allow 3D mitochondrial dynamics to be sampled and extracted almost in real time. In this work, mitochondrial fluorescence excited by the PNAs was captured by an optical microscope using dual objective lenses, which produced superlocalized dynamics while minimizing light scattering by the plasmonic substrate. It was found that mitochondria move with an average velocity 0.33 ± 0.26 μm/s, a significant part of which, by almost 50%, was contributed by the movement along the depth axis (z-axis). Mitochondrial positions were acquired with superlocalized precision (σ x = 5.7 nm and σ y = 11.8 nm) in the lateral plane and σ z = 78.7 nm in the z-axis, which presents an enhancement by 12.7-fold in resolution compared to confocal fluorescence microscopy. The approach is expected to serve as a way to provide 3D information on molecular dynamics in real time.

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Section: Methodsmentioning

confidence: 99%