Controlling electroosmotic flow in capillary zone electrophoresis

Cohen, Noa; Grushka, Eli

doi:10.1016/0021-9673(94)87086-1

Cited by 46 publications

(17 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…pH 9.0. This is consistent with previous results that the EOF obtained with fused-silica capillary undergoes a rapid decrease as the buffer pH is decreased in the pH region corresponding to the silanol pK a of about 5.9 [18,19]. The sulfonated capillary was prepared with CSPECS, which is trifunctional reagent, as shown in Fig.…”

Section: Characterization Of the Sulfonated Capillarysupporting

confidence: 71%

“…The behavior of the EOF most likely mirrors the concentration of the ionized silanol groups on the capillary surface. Thus, by measuring the EOF as a function of the pH, the titration of the capillary surface silanols can be monitored [18]. An effective way to measure changes on the inner wall surface of the capillary induced by chemical reactions is to determine the magnitude and the direction of the EOF, which is generally viewed as the major characteristic property of the coating [4,17].…”

Section: Characterization Of the Sulfonated Capillarymentioning

confidence: 99%

See 1 more Smart Citation

A sulfonated capillary that gives reproducible migration times for capillary zone electrophoresis and micellar electrokinetic chromatography

Kodama

Yamamoto

Terashima³

et al. 2005

Electrophoresis

View full text Add to dashboard Cite

To obtain reproducible migration times and rapid analyses of analytes, sulfonate groups were chemically introduced to the inner wall of untreated fused-silica capillary with 2-(4-chlorosulfonylphenyl)ethyltrichlorosilane. The sulfonated capillary showed relatively constant electroosmotic mobility which was greater than that obtained by an untreated fused-silica capillary over the pH range studied (pH 2-9). In both CZE and MEKC, the RSDs of the migration times of analytes with the sulfonated capillary were less than 0.2% which were significantly lower than those obtained with an untreated fused-silica capillary (0.5-3.5%). When BGE were set at pH 7.0 for CZE and MEKC, the analysis times with the sulfonated capillary were about half those obtained with an untreated fused-silica capillary. These results indicate that the sulfonated capillary can provide highly reproducible and rapid analyses in CE.

show abstract

Section: Characterization Of the Sulfonated Capillarysupporting

confidence: 71%

Section: Characterization Of the Sulfonated Capillarymentioning

confidence: 99%

A sulfonated capillary that gives reproducible migration times for capillary zone electrophoresis and micellar electrokinetic chromatography

Kodama

Yamamoto

Terashima³

et al. 2005

Electrophoresis

View full text Add to dashboard Cite

show abstract

“…In DNA separation via this method it is important that resolution and speed can be further optimized by regulating EOF through control of charge and composition of the capillary wall [20]. Factors affecting EOF, such as pH, ionic strength and composition of the running buffers, are considered in several studies [21±24].…”

Section: Introductionmentioning

confidence: 99%

A new strategy for optimizing sensitivity, speed, and resolution in capillary electrophoretic separation of DNA

Tseng

Chang

2001

Electrophoresis

View full text Add to dashboard Cite

DNA separations were performed in poly(ethylene oxide) (PEO) solutions prepared in 100 mM Tris-boric acid (TB) buffers using a capillary filled with TB buffers with concentrations up to 2.5 M, pH 10.0. The electroosmotic flow (EOF) increased with increasing the concentration of TB buffers till 1.5 M as a result of decreasing PEO adsorption on the capillary wall. At high TB concentrations (> 1.5 M), the peaks corresponding to small DNA fragments (11 and 8 base pairs) became sharper and were detected. Relative standard deviations of the EOF coefficient and the migration times of the DNA fragments were all less than 1% using a capillary filled with TB buffers at concentrations higher than 1.5 M. When separations were performed at different pH values of PEO solutions and TB buffers, better results in terms of sensitivity, speed, and resolution were generally achieved. The fluorescence intensity of the 2176 bp fragment obtained at pH values of TB buffers/PEO solutions 10.0/8.2 was 27-fold of that at pH values 8.2/8.2. The enhancement was related to effects of pH and borate on fluorescence intensity, DNA conformation, stacking, and interactions with the capillary wall. Using a capillary filled with 400 mM TB buffers, pH 10.0, the separation of DNA (pBR 322/HaeIII digest, pBR 328/Bg/I digest and pBR 328/HinfI digest) in 1.5% PEO solutions prepared in 100 mM TB buffers, pH 9.0, at 375 V/cm was accomplished in less than 18 min.

show abstract

“…Capillary rinsing can make the system return to the initial running conditions, so the influence of the EOF variation is reduced [13][14]. The total rinsing time was set to 10.0 min in order to compare efficiency of rinsing methods under the same time consumption.…”

Section: Effect Of Capillary Rinse Proceduresmentioning

confidence: 99%

Development and Validation of Improved Method for Fingerprint Analysis of <i>Rhizoma Chuanxiong</i> by Capillary Zone Electrophoresis with Ultraviolet-Diode Array Detection

Wei¹,

Shen²,

Chen³

2014

Trop. J. Pharm Res

View full text Add to dashboard Cite

Controlling electroosmotic flow in capillary zone electrophoresis

Cited by 46 publications

References 24 publications

A sulfonated capillary that gives reproducible migration times for capillary zone electrophoresis and micellar electrokinetic chromatography

A sulfonated capillary that gives reproducible migration times for capillary zone electrophoresis and micellar electrokinetic chromatography

A new strategy for optimizing sensitivity, speed, and resolution in capillary electrophoretic separation of DNA

Development and Validation of Improved Method for Fingerprint Analysis of <i>Rhizoma Chuanxiong</i> by Capillary Zone Electrophoresis with Ultraviolet-Diode Array Detection

Contact Info

Product

Resources

About