Controlling Brochothrix thermosphacta as a spoilage risk using in-package atmospheric cold plasma

Patange, Apurva; Boehm, Daniela; Bueno-Ferrer, Carmen; Cullen, Patrick J.; Bourke, Paula

doi:10.1016/j.fm.2017.04.002

Cited by 55 publications

(24 citation statements)

References 39 publications

(42 reference statements)

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“…The current research uses an experimental dielectric barrier discharge (DBD) plasma device, DIT 120, with a maximum voltage output of 120 kV at 50 Hz generated between two 15 cm diameter aluminium disk electrodes (Fig. 1a,b ) 20 , 21 . We have previously characterised biological activities in cancer cells that are dependent and independent of reactive species generation using this system 22 , 23 .…”

Section: Introductionmentioning

confidence: 99%

Cold Atmospheric Plasma Induces ATP-Dependent Endocytosis of Nanoparticles and Synergistic U373MG Cancer Cell Death

He¹,

Liu²,

Manaloto³

et al. 2018

Sci Rep

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Gold nanoparticles (AuNP) have potential as both diagnostic and therapeutic vehicles. However, selective targeting and uptake in cancer cells remains challenging. Cold atmospheric plasma (CAP) can be combined with AuNP to achieve synergistic anti-cancer cytotoxicity. To explore synergistic mechanisms, we demonstrate both rate of AuNP uptake and total amount accumulated in U373MG Glioblastoma multiforme (GBM) cells are significantly increased when exposed to 75 kV CAP generated by dielectric barrier discharge. No significant changes in the physical parameters of AuNP were caused by CAP but active transport mechanisms were stimulated in cells. Unlike many other biological effects of CAP, long-lived reactive species were not involved, and plasma-activated liquids did not replicate the effect. Chemical effects induced by direct and indirect exposure to CAP appears the dominant mediator of enhanced uptake. Transient physical alterations of membrane integrity played a minor role. 3D-reconstruction of deconvoluted confocal images confirmed AuNP accumulation in lysosomes and other acidic vesicles, which will be useful for future drug delivery and diagnostic strategies. Toxicity of AuNP significantly increased by 25-fold when combined with CAP. Our data indicate that direct exposure to CAP activates AuNP-dependent cytotoxicity by increasing AuNP endocytosis and trafficking to lysosomes in U373MG cells.

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Section: Introductionmentioning

confidence: 99%

Cold Atmospheric Plasma Induces ATP-Dependent Endocytosis of Nanoparticles and Synergistic U373MG Cancer Cell Death

He¹,

Liu²,

Manaloto³

et al. 2018

Sci Rep

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“…The use of cold atmospheric plasma (CAP) has attracted a lot of attention over the past few years and has already found many biomedical applications, including wound healing [1] , sterilization [2] , biofilm removal [3] , blood coagulation [4] , and more recently cancer therapy [5] . CAP is a plasma (i.e., ionized gas) in which the high-temperature electrons are in non-equilibrium with the low-temperature heavy particles, thereby keeping the gas itself near room temperature and making it suitable for treatment of living cells and tissues.…”

Section: Introductionmentioning

confidence: 99%

The effect of reactive oxygen and nitrogen species on the structure of cytoglobin: A potential tumor suppressor

et al. 2018

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Many current anti-cancer therapies rely on increasing the intracellular reactive oxygen and nitrogen species (RONS) contents with the aim to induce irreparable damage, which subsequently results in tumor cell death. A novel tool in cancer therapy is the use of cold atmospheric plasma (CAP), which has been found to be very effective in the treatment of many different cancer cell types in vitro as well as in vivo, mainly through the vast generation of RONS. One of the key determinants of the cell's fate will be the interaction of RONS, generated by CAP, with important proteins, i.e. redox-regulatory proteins. One such protein is cytoglobin (CYGB), a recently discovered globin proposed to be involved in the protection of the cell against oxidative stress. In this study, the effect of plasma-produced RONS on CYGB was investigated through the treatment of CYGB with CAP for different treatment times. Spectroscopic analysis of CYGB showed that although chemical modifications occur, its secondary structure remains intact. Mass spectrometry experiments identified these modifications as oxidations of mainly sulfur-containing and aromatic amino acids. With longer treatment time, the treatment was also found to induce nitration of the heme. Furthermore, the two surface-exposed cysteine residues of CYGB were oxidized upon treatment, leading to the formation of intermolecular disulfide bridges, and potentially also intramolecular disulfide bridges. In addition, molecular dynamics and docking simulations confirmed, and further show, that the formation of an intramolecular disulfide bond, due to oxidative conditions, affects the CYGB 3D structure, thereby opening the access to the heme group, through gate functioning of His117. Altogether, the results obtained in this study (1) show that plasma-produced RONS can extensively oxidize proteins and (2) that the oxidation status of two redox-active cysteines lead to different conformations of CYGB.

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“…Investigations on improving food shelf-life through atmospheric cold plasma have considered ready-to-eat foods such as fresh fruit and vegetables [28,29] and meat [30,31].…”

Section: Decontamination For Shelf-life Extensionmentioning

confidence: 99%

The Potential of Cold Plasma for Safe and Sustainable Food Production

Bourke¹,

Ziuzina²,

Boehm³

et al. 2018

Trends in Biotechnology

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302

183

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Cold plasma science and technology is increasingly investigated for translation to a plethora of issues in the agriculture and food sectors. The diversity of the mechanisms of action of cold plasma, and the flexibility as a standalone technology or one that can integrate with other technologies, provide a rich resource for driving innovative solutions. The emerging understanding of the longer-term role of cold plasma reactive species and follow-on effects across a range of systems will suggest how cold plasma may be optimally applied to biological systems in the agricultural and food sectors. Here we present the current status, emerging issues, regulatory context, and opportunities of cold plasma with respect to the broad stages of primary and secondary food production.

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Controlling Brochothrix thermosphacta as a spoilage risk using in-package atmospheric cold plasma

Abstract: Brochothrix thermosphacta is a predominant spoilage microorganism in meat and its control 12 in processing environments is important to maintain meat product quality. Atmospheric cold

Cited by 55 publications

References 39 publications

Cold Atmospheric Plasma Induces ATP-Dependent Endocytosis of Nanoparticles and Synergistic U373MG Cancer Cell Death

Cold Atmospheric Plasma Induces ATP-Dependent Endocytosis of Nanoparticles and Synergistic U373MG Cancer Cell Death

The effect of reactive oxygen and nitrogen species on the structure of cytoglobin: A potential tumor suppressor

The Potential of Cold Plasma for Safe and Sustainable Food Production

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