Controlled vitamin D delivery with injectable hyaluronic acid-based hydrogel for restoration of tendinopathy

Abstract

Tendinopathy is a term used to describe tendon disorders that are marked by pain and a loss of function. Recent studies demonstrated that inflammation plays an important role throughout the broad spectrum of tendinopathy. Conventional treatments such as steroid injections, analgesics, and physical modalities simply give pain relief and do not alter the disease progression without the tendon regeneration effect. Tenocytes are responsible for maintaining the tendon matrix and understanding how they function is essential to studying new treatments for tendinopathy. Our previous study showed the protective effects of vitamin D (Vit D) on damaged tenocytes. Besides its well-known effects on bone metabolism, the non-classical action of Vit D is the pleiotropic effects on modulating immune function. In the present study, we developed a Vit D delivery system with hyaluronic acid (HA), which is one of the major components of the extracellular matrix that has anti-inflammation and wound-healing properties. A novel Vit D delivery system with cross-linked HA hydrogel (Gel) and Tween 80 (T80), Vit D@Gel/T80, could be a new regeneration technique for the treatment of tendinopathy. Vit D@Gel/T80 reduced TNF-α induced damage to human tenocytes in vitro. In an animal study, the Vit D@Gel/T80 injected group demonstrated tendon restoration features. As a result, this Vit D@Gel/T80 system might be a local injection material in the treatment for tendinopathy.

Multi-modulation of immune-inflammatory response using bioactive molecule-integrated PLGA composite for spinal fusion

“…1 A). To fabricate homogeneously PDRN-dispersed PLGA composite, Span 80 (a water in oil emulsifier) was used to form a nano-emulsion in organic solvent [ 26 ]. Surface morphological properties were investigated using scanning electron microscopy.…”

“…However, PDRN is water-soluble and can aggregate in organic solvents. Therefore, to fabricate homogeneous dispersion in hydrophobic PLGA composite, we adopted a nano-emulsion-based dispersion method using Span 80 [ 26 ].…”

Fibrous hydrogels by electrospinning: Novel platforms for biomedical applications

“…To meet needs of improvement in cell culture platforms, hydrogels to mimic ECM and microfabrication techniques to create in vivo-like microenvironments were applied to develop the platforms. Cells were grown on various hydrogels, [99][100][101][102][103][104][105] and cell differentiation and migration were studied by analyzing cells on hydrogels with stiffness alteration. 106,107 Microfluidic chips providing in vivo-like cell culture platforms were also used to study various cellular mechanisms [108][109][110][111] and even developed as organ-on-achip by mimicking features of specific organs.…”

Disc regeneration by injectable fucoidan-methacrylated dextran hydrogels through mechanical transduction and macrophage immunomodulation

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