Controlled sealing of nanopores using an easily fabricated silicon platform combining in situ optical and electrical monitoring

Nanoparticle Translocation through Conical Nanopores: A Finite Element Study of Electrokinetic Transport

“…It might be possible to overcome this effect by applying a hydrostatic pressure difference between the two reservoirs, a salt density difference, a temperature difference, or a magnetic field . Alternatively, methods characterizing trapped instead of translocating particles might be possible . Second, the signal strength—defined as the change in electric current through the pore during the translocation, with respect to the base current without the sphere—is largest when a positive voltage ( E ‐field pointing out of the pore orifice into the bulk) is applied at high ionic strength or a negative voltage is applied at low ionic strength.…”

Electrical trapping mechanism of single-microparticles in a pore sensor

“…On the other hand, combining optical microscopy observations with nanopore measurements have proven to be an effective technique to address this issue: fast translocation of dyed particles or molecules through a fluidic channel are traced by fluorescence imaging while recording the cross-pore ionic current simultaneously so that the ionic current signals obtained can be assigned unambiguously to the actual events occurred. [22][23][24][25][26][27] Mitsui et al 22 studied electrophoretic capture of single-molecule DNA into a nanopore by fluorescent observations alone. They observed motion of DNA around a nanopore with different gate voltages and presented the possibility for controlling the trajectory of analyte at the vicinity of a nanopore.…”

“…They observed motion of DNA around a nanopore with different gate voltages and presented the possibility for controlling the trajectory of analyte at the vicinity of a nanopore. Furthermore, several groups [23][24][25][26][27] have succeeded in detections of analyte translocations by simultaneous measurements of the ionic current and the fluorescence imaging. As described above, such simultaneous measurement utilizing fluorescent observation facilitates to conform the relationships between the experimental condition and dynamics of analyte and develops reliable high-throughput nanoscale device based on rich information of dynamic behavior of the targets.…”