Controlled release systems for DNA delivery

Pannier, Angela K.

doi:10.1016/s1525-0016(04)00123-6

Cited by 55 publications

(84 citation statements)

References 82 publications

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“…Atomic force microscopy imaging of immobilized complexes supports this hypothesis, revealing that the presence of PEG on the surface significantly affects the morphology of the complexes, which correlates with greater high transfection levels and transfection efficiencies. The ability to control the morphology of the immobilized complexes and thus influence transfection levels could be translated to scaffolds for gene delivery in tissue engineering applications [5,6], as well other applications of substrate-mediated gene delivery, including transfected cell arrays [63]. Cell adhesion on EG-containing SAMs.…”

Section: Discussionmentioning

confidence: 99%

“…Controlled release systems for DNA delivery have the potential to overcome extracellular barriers that limit gene transfer and enhance gene delivery relative to more traditional delivery methods [5]. These systems include delivery through polymeric release, in which the DNA is released from a polymer scaffold, or substrate-mediated delivery, in which DNA is retained at the surface of a substrate.…”

Section: Introductionmentioning

confidence: 99%

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Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes

2008

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Substrate-mediated gene delivery describes the immobilization of gene therapy vectors to a biomaterial, which enhances gene transfer by exposing adhered cells to elevated DNA concentrations within the local microenvironment. Surface chemistry has been shown to affect transfection by nonspecifically immobilized complexes using self-assembled monolayers (SAMs) of alkanethiols on gold. In this report, SAMs were again used to provide a controlled surface to investigate whether the presence of oligo(ethylene glycol) (EG) groups in a SAM could affect complex morphology and enhance transfection. EG groups were included at percentages that did not affect cell adhesion. Nonspecific complex immobilization to SAMs containing combinations of EG-and carboxylic acidterminated alkanethiols resulted in substantially greater transfection than surfaces containing no EG groups or SAMs composed of EG groups combined with other functional groups. Enhancement in transfection levels could not be attributed to complex binding densities or release profiles. Atomic force microscopy imaging of immobilized complexes revealed that EG groups within SAMs affected complex size and appearance and could indicate the ability of these surfaces to preserve complex morphology upon binding. The ability to control the morphology of the immobilized complexes and influence transfection levels through surface chemistry could be translated to scaffolds for gene delivery in tissue engineering and diagnostic applications.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes

2008

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“…Controlled delivery systems, including polymeric release in which the DNA is released from the polymer, or substrate-mediated delivery, in which DNA is retained at the surface, have the potential to overcome extracellular barriers that limit gene transfer, as well as enhance gene delivery relative to more traditional delivery methods [6]. In substrate-mediated delivery, also termed reverse transfection or solid-phase delivery, plasmid DNA or DNA complexes are immobilized to a surface or biomaterial that supports cell adhesion.…”

Section: Introductionmentioning

confidence: 99%

Substrate-mediated delivery from self-assembled monolayers: Effect of surface ionization, hydrophilicity, and patterning

2005

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Gene transfer has many potential applications in basic and applied sciences. In vitro, DNA delivery can be enhanced by increasing the concentration of DNA in the cellular microenvironment through immobilization of DNA to a substrate that supports cell adhesion. Substrate-mediated delivery describes the immobilization of DNA, complexed with cationic lipids or polymers, to a biomaterial or substrate. As surface properties are critical to the efficiency of the surface delivery approach, selfassembled monolayers (SAMs) of alkanethiols on gold were used to correlate surface chemistry of the substrate to binding, release, and transfection of non-specifically immobilized complexes. Surface hydrophobicity and ionization were found to mediate both DNA complex immobilization and transfection, but had no effect on complex release. Additionally, SAMs were used in conjunction with soft lithographic techniques to imprint substrates with specific patterns, resulting in patterned DNA complex deposition and transfection, with transfection efficiencies in the patterns nearing 40%. Controlling the interactions between complexes and substrates, with the potential for patterned delivery, can be used to locally enhance or regulate gene transfer, with applications to tissue engineering scaffolds and transfected cell arrays.

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“…Direct delivery of naked DNA can induce transgene expression in vivo; however, plasmids are rapidly degraded by DNase and are rapidly cleared from the tissue [4,5]. Controlled release systems can protect plasmid from nucleases, and a sustained release can replace DNA that is cleared or degraded thereby maintaining elevated levels within the tissue [6][7][8][9][10][11]. These controlled release systems often involve the use of biodegradable polymers to encapsulate the plasmid.…”

Section: Introductionmentioning

confidence: 99%

Intramuscular delivery of DNA releasing microspheres: Microsphere properties and transgene expression

Jang

Shea²

2006

Journal of Controlled Release

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Plasmid-loaded microspheres can provide localized and sustained release into the target tissue, and thus have the potential to enhance the efficiency of naked DNA at promoting transgene expression. In this report, microsphere design parameters are investigated by correlating the extent and duration of transgene expression intramuscularly to the polymer molecular weight and the mass of DNA delivered. Plasmid DNA was incorporated into poly (lactide-co-glycolide) microspheres using a cryogenic double emulsion process, and microspheres were injected intramuscularly. Bolus injection of naked plasmid was used for control, which exhibited transfection of muscle cells with transgene expression that gradually decreased over time. Microspheres fabricated from low molecular weight polymer had expression levels that increased from day 1 to day 92, which subsequently decreased through day 174. Decreasing the microsphere mass delivered resulted in steady expression during the same time. However, microspheres fabricated with high molecular weight polymer had expression for only 14 days. Intramuscular injection resulted in a foreign body response to the microspheres, and these infiltrating cells adjacent were primarily transfected. This understanding of microsphere properties that determine transgene expression and the distribution of transfected cells may facilitate their application to fields such as tissue engineering or DNA vaccines.

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Controlled release systems for DNA delivery

Cited by 55 publications

References 82 publications

Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes

Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes

Substrate-mediated delivery from self-assembled monolayers: Effect of surface ionization, hydrophilicity, and patterning

Intramuscular delivery of DNA releasing microspheres: Microsphere properties and transgene expression

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